Establish and Characterize an Air Liquid Interface In Vitro Exposure System

体外暴露系统气液界面的建立和表征

• 批准号: 10599064
• 负责人: Barney Sparrow
• 金额: $ 124.41万
• 依托单位: BATTELLE MEMORIAL INSTITU
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-15 至 2023-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10599064
• 关键词: Aerosols; Air; Air Pollutants; Animals; Cells; Complex Mixtures; Data; Data Correlations; Evaluation; Fiber; Goals; Government; Health Hazards; Human; In Vitro; Industrialization; Inhalation; Inhalation Exposure; Liquid substance; Lung; Population; Powder dose form; Rattus; System; Testing; Tissues; Toxicity Tests; Toxicology; design; exposure route; in vitro testing; in vivo; pilot test; programs; toxicant; vapor

The Division of Translational Toxicology (DTT) continued to optimize use of the government owned Vitrocell 48 2.0 plus exposure system to provide the program with in vitro capabilities that mimic inhalation exposure. The inhalation route of exposure is commonly seen among air pollutants as well as with industrial settings, and until recently has been very difficult to simulate in vitro. This system allows in vitro testing of potential toxicants for inhalation exposure which contributes to the program's goals of reducing the use of animals. This system provides high throughput and can be used for airborne substances such as vapors, complex mixtures of liquid aerosol, dry powders, and fibers. DTT is conducting additional evaluation of the system to increase confidence that the system will function as intended. This will be achieved through the design and conduct of system characterization and pilot in vitro toxicity testing of selected test agents with robust in vivo data for correlation, using tissue from representative human populations. The system also allows testing of cells from humans and rats to provide data supporting species extrapolation. Keyword: in vitro, inhalation exposure

转化毒理学（DTT）的分区继续优化政府拥有的Vitrocell 48 2.0加曝光系统的使用，以提供该计划的体外能力，以模仿吸入暴露。在空气污染物和工业环境中，通常可以看到接触的吸入途径，直到最近，很难在体外模拟。该系统允许对潜在有毒物质吸入暴露的体外测试，这有助于该计划减少动物使用的目标。该系统可提供高吞吐量，可用于机载物质，例如蒸气，液体气溶胶，干粉末和纤维的复杂混合物。 DTT正在对系统进行其他评估，以提高对系统将按预期运行的信心。这将通过使用代表性人群的组织对所选测试剂进行系统表征的设计和进行体外毒性测试来实现这一目标。该系统还允许对人类和大鼠的细胞进行测试，以提供支持物种外推的数据。 关键字：体外，吸入暴露