Calcium coding mechanisms in plant cell growth and immunity

植物细胞生长和免疫中的钙编码机制

• 批准号: 10581257
• 负责人: Sheng Luan
• 金额: $ 6.67万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10581257
• 关键词: Animals; Arabidopsis; Calcium; Calcium Signaling; Code; Containment; Controlled Environment; DNA; Ensure; Equipment; Eukaryota; Floods; Floor; Foundations; Funding; Future; Genetically Modified Organisms; Growth; Health; Human; Immune response; Immunity; Maintenance; Physiological Processes; Plant Model; Plants; Preventive; Second Messenger Systems; Solid; Specific qualifier value; Transgenic Organisms; Universities; cell growth; genetic approach; human old age (65+); neuron development; new growth; parent project; pathogen; pathogenic bacteria; plant growth/development; programs

This equipment supplement application seeks funding for replacement of a broken growth chamber for culturing plant materials used in the parent project. The model plant Arabidopsis must be grown and maintained in a controlled environments. In particular, our plants are routinely transgenic containing foreign DNAs and considered “genetically modified organisms” (GMOs) and thus must be contained in a specified chamber. We are also working with pathogenic bacteria that induce immune responses in plants. Such pathogens also require containment. We have been using the Conviron plant growth chambers for these critical purposes. We had two old growth chambers (27 years) at the beginning of the parent project. An accidental floor flooding and leak above the growth chamber facility occurred and one of the growth chambers was severely damaged and we requested funding through the same supplement program in 2021 to replace it. In February 2022, the other old chamber failed due to old age. Two growth chambers are minimally required to grow plants with and without pathogen treatments. I thus request funding herein to purchase a new chamber to replace the failed chamber to ensure steady progress of the project and reduce the chance of cross-contamination of plant materials. To ensure the growth chamber operates correctly and are maintained properly, we propose to purchase the growth chamber from the same company Conviron so that our lab is familiar with the use of the chamber. Our University greenhouse and facility staff team will commit to the preventive maintenance of the growth chamber. We expect to have the new chamber in use as early as August 2022 should the funding become available in July. The two new growth chambers will provide a solid foundation for supplying plant materials in this funding period and future renewal of the project.

该设备补充申请寻求替换增长破裂的资金 培养植物材料的室内材料。 必须在受控环境中生长和维护。 常规的转基因含有外国DNA并同意“遗传修饰” 有机体”（GMO），因此必须包含在指定的腔室中。我们也在工作 诱导植物中免疫反应的致病细菌 需要遏制。 关键目的。 项目。 一个成长室之一受到了损害，我们要求通过 2021年2月，另一个旧房间在2021年取代了相同的补充计划。 由于老年而失败。 没有病原体治疗。 替换失败的会议厅以确保项目的稳定进展并减少机会 植物材料的交叉污染。 为了确保生长室正确运行并正确维护，我们 建议从同一公司Conviron购买增长室，以便我们的实验室 熟悉我们的大学温室和设施员工团队的使用 致力于预防增长室。 在Asugust 2022年早期使用的商会将于7月提供资金 两个新的生长室将为提供植物材料提供坚实的基础 该项目的资金期和未来续约。