Molecular Cytology

分子细胞学

基本信息

批准号：
10571803
负责人：
katia Ognianova manova
金额：
$ 47.71万
依托单位：
SLOAN-KETTERING INST CAN RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-01-20 至 2023-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10571803
关键词：
Animals Antibodies Area Behavior Binding Biological Markers Cancer Center Cancer Center Support Grant Cancer Patient Cells Collaborations Cytology Data Data Analyses Detection Disease Embryo Ensure Fluorescence Glycoproteins Histology Image Image Analysis Imaging Device Immunofluorescence Immunologic Immunohistochemistry In Situ Lipids Malignant Neoplasms Manuals Methodology Microscopy Mission Molecular Mus Normal tissue morphology Nucleic Acids Organoids Pathologic Pathologic Processes Pathway interactions Pharmaceutical Preparations Preparation Process Proteins Protocols documentation Reproducibility of Results Research Research Personnel Research Project Grants Research Support Resources Sampling Scanning Scanning Probe Microscopes Services Slide Stains Technology Thick Three-Dimensional Image Tissue Sample Tissue imaging Tissues Training Validation Visualization confocal imaging digital experimental study image processing imaging capabilities imaging modality inhibitor investigator training member molecular marker multiplex detection nanomaterials nanometer resolution optical imaging spectroscopic imaging superresolution imaging tissue preparation tumor

项目摘要

ABSTRACT The mission of the Molecular Cytology Core is to deliver cutting-edge, specialized services in support of research projects at the Center. The methodologies employed by the Core allow for the detection in situ (in cells, tissue sections, organoids and whole mounts) of biomarkers (proteins, glycoproteins, lipids, nucleic acids) and molecular processess in normal and pathological conditions. The precise localization of the biomarkers is achieved by optical imaging of fixed and live samples. Several molecular markers (up to seven) can be vizualized simultanously or sequentially in the same sample by multiplex staining, using machine-based protocols. In collaboration with its users, the Core performs comprehensive antibody validations. The Core trains investigators in basic staining principles and assists them in performing manual experiments. The optical imaging modalities within the Core allow for wide field imaging and confocal imaging of tissue sections, thick and cleared tissue samples, cells, live embryos, tumors in live mice, etc. Super-resolution imaging is performed as well as confocal imaging in the IR range to visualize nanomaterials and drugs. With the newly acquired module for fluorescence correlation spectroscopy (FCS) imaging, researchers can study the dynamic behavior of biomolecules. The Core scans user slides with four digital slide scanners, one of which has confocal capabilities. The scanning generates large volumes of data and serves as a valuable resource for image analysis. Microscopy staff provides strong support for the users with 2D and 3D image analysis. The Core’s Atomic Force microscope is heavily used to study molecular interactions with nanometer resolution, to evaluate the effect of inhibitors on pathologic processes, and to assess stiffness of tissues from normal and cancer- bearing animals as well as tissues from cancer patients.

抽象的分子细胞学核心的使命是提供尖端的专业服务以支持该中心的研究项目允许进行原位检测。生物标志物（蛋白质、糖蛋白、脂质、核酸、细胞、组织切片、类器官和整体）酸）和正常和病理条件下的分子过程。生物标记是通过固定样品和活体样品的光学成像来实现的，其中包括多种分子标记（最多七个）。可以使用基于机器的多重染色同时或顺序地在同一样品中进行可视化 Core 与用户合作执行全面的抗体验证。培训研究人员基本染色原理并协助他们进行手动实验。 Core 内的成像方式允许对组织切片、厚切片进行宽视野成像和共焦成像并清除组织样本、细胞、活体胚胎、活体小鼠肿瘤等，进行超分辨率成像以及红外范围内的共焦成像，以可视化纳米材料和药物。荧光相关光谱 (FCS) 成像模块，研究人员可以研究动态行为 Core 使用四台数字载玻片扫描仪扫描用户载玻片，其中一台具有共焦功能。扫描会生成大量数据，并作为图像的宝贵资源。显微镜工作人员为用户提供 2D 和 3D 图像分析的有力支持。原子力显微镜广泛用于研究纳米分辨率的分子相互作用，以评估抑制剂对病理过程的影响，并评估正常组织和癌症组织的硬度生育动物以及癌症患者的组织。