Melecular Cytology

分子细胞学

• 批准号: 8933517
• 负责人: katia Ognianova manova
• 金额: $ 46.06万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8933517
• 关键词: Animals; Antibodies; Basic Science; Behavior; Biological; Calcium ion; Cancer Center; Cancer Center Support Grant; Cells; Cellular biology; Clinical; Clinical Research; Communities; Computer software; Core Facility; Cues; Cytology; Detection; Equipment; Faculty; Fluorescence Resonance Energy Transfer; Grant; Heterogeneity; Histology; Housing; Image; Image Analysis; Imaging Device; Immunohistochemistry; In Situ; Individual; Instruction; Malignant Neoplasms; Manuals; Memorial Sloan-Kettering Cancer Center; Microscope; Microscopy; Modeling; Molecular; Neurons; Nucleic Acids; Optics; Patients; Pattern; Proteins; Publications; Research; Research Personnel; Research Support; Resolution; Resources; Sampling; Scanning; Services; Staging; Time; Tissues; Training; Tumor Tissue; Work; cancer cell; cancer genetics; digital; image processing; imaging modality; instrumentation; member; nerve stem cell; optical imaging; programs; relating to nervous system; research study; tumor; Animals; Antibodies; Basic Science; Behavior; Biological; Calcium ion; Cancer Center; Cancer Center Support Grant; Cells; Cellular biology; Clinical; Clinical Research; Communities; Computer software; Core Facility; Cues; Cytology; Detection; Equipment; Faculty; Fluorescence Resonance Energy Transfer; Grant; Heterogeneity; Histology; Housing; Image; Image Analysis; Imaging Device; Immunohistochemistry; In Situ; Individual; Instruction; Malignant Neoplasms; Manuals; Memorial Sloan-Kettering Cancer Center; Microscope; Microscopy; Modeling; Molecular; Neurons; Nucleic Acids; Optics; Patients; Pattern; Proteins; Publications; Research; Research Personnel; Research Support; Resolution; Resources; Sampling; Scanning; Services; Staging; Time; Tissues; Training; Tumor Tissue; Work; cancer cell; cancer genetics; digital; image processing; imaging modality; instrumentation; member; nerve stem cell; optical imaging; programs; relating to nervous system; research study; tumor

The Molecular Cytology Core Facility (MCCF) underpins all the basic and clinical research at MSKCC involving the interrogation of proteins or nucleic acids in cells, tissues and tumors. The MCCF retains state-of- the-art equipment and an array of imaging tools and a team of highly talented technical assistants and as such provides both a service and one-on-one training enabling faculty and their trainees to work independently with high-end instrumentation. A resource of validated antibodies for automated or manual histology is a feature of the Core's rapid and reliable service used by investigators working with animal tumor models or patient samples. To explore the cell biology of cancer cells at high spatial and temporal resolution, the Core's in-house expertise enables the power of a superb suite of confocal microscopes to be accessible not only to cell biologists but also to the wider community of members of the Cancer Center. Imaging modalities for molecular detection available to the researchers at the MCCF are optical microscopes, including wide field (epifluorescence, bright field, polarizing and DIC), confocal (raster scanning, line scanning and spinning disc), time lapse microscopy, FLIM and digital scanners. Experiments involving uncaging experiments, FRAP and FRET, and Ratio imaging of calcium ions are also carried out at the MCCF. In addition to assistance and training on image acquisition, the MCCF staff provide assistance to researchers in image processing and analysis using Velocity, MetaMorph, Imaris and MatLab software. The broad range of services and collaborative work provided by the Molecular Cytology Core has supported the research of 120 investigators in the past year. During the past grant period the work of the Core has contributed to 315 publications of researchers from 5 research programs. For example, with the assistance of the Core, Studer and Tabar demonstrated that neural rosette cells represent the first characterized neural stem cell stage capable of responding to patterning cues that direct differentiation toward region-specific neuronal fates. The Core provided immunohistochemistry and image analysis critical to this work.

分子细胞学核心设施（MCCF）为MSKCC的所有基本和临床研究的基础 涉及细胞，组织和肿瘤中蛋白质或核酸的询问。 MCCF保留 Art设备和一系列成像工具以及一支由才华横溢的技术助理组成的团队 这样的服务提供了服务和一对一的培训，使教师及其学员可以工作 独立使用高端仪器。自动或手册的经过验证抗体的资源 组织学是核心快速可靠的服务的特征 模型或患者样品。探索在高空间和时间上的癌细胞的细胞生物学 解决方案，核心的内部专业知识使精湛的共聚焦显微镜的力量能够成为 不仅可以访问细胞生物学家，而且可以访问癌症中心成员的更广泛社区。 MCCF研究人员可用于分子检测的成像方式是光学的 显微镜，包括宽场（落荧光，明亮场，偏振和DIC），共焦（栅格 扫描，线条扫描和旋转盘），延时显微镜，FLIM和数字扫描仪。实验 还涉及分离实验，FRAP和FRET以及钙离子的比率成像 MCCF。除了获得图像获取的协助和培训外，MCCF的工作人员还为 使用速度，Metamorph，Imaris和Matlab软件进行图像处理和分析的研究人员。这 分子细胞学核心提供的广泛服务和协作工作已支持 过去一年对120名调查人员的研究。在过去的赠款期间，核心的工作已经 为5个研究计划的315名研究人员出版物做出了贡献。例如，在协助下 在核心中，Studer和Tabar证明了神经玫瑰花塞细胞代表了第一个特征的神经 干细胞阶段，能够响应直接分化为特定区域的图案提示 神经元的命运。核心提供了对这项工作至关重要的免疫组织化学和图像分析。