Melecular Cytology

分子细胞学

基本信息

批准号：
8933517
负责人：
katia Ognianova manova
金额：
$ 46.06万
依托单位：
SLOAN-KETTERING INST CAN RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-01-01 至 2018-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8933517
关键词：
Animals Antibodies Basic Science Behavior Biological Calcium ion Cancer Center Cancer Center Support Grant Cells Cellular biology Clinical Clinical Research Communities Computer software Core Facility Cues Cytology Detection Equipment Faculty Fluorescence Resonance Energy Transfer Grant Heterogeneity Histology Housing Image Image Analysis Imaging Device Immunohistochemistry In Situ Individual Instruction Malignant Neoplasms Manuals Memorial Sloan-Kettering Cancer Center Microscope Microscopy Modeling Molecular Neurons Nucleic Acids Optics Patients Pattern Proteins Publications Research Research Personnel Research Support Resolution Resources Sampling Scanning Services Staging Time Tissues Training Tumor Tissue Work cancer cell cancer genetics digital image processing imaging modality instrumentation member nerve stem cell optical imaging programs relating to nervous system research study tumor

项目摘要

The Molecular Cytology Core Facility (MCCF) underpins all the basic and clinical research at MSKCC involving the interrogation of proteins or nucleic acids in cells, tissues and tumors. The MCCF retains state-of- the-art equipment and an array of imaging tools and a team of highly talented technical assistants and as such provides both a service and one-on-one training enabling faculty and their trainees to work independently with high-end instrumentation. A resource of validated antibodies for automated or manual histology is a feature of the Core's rapid and reliable service used by investigators working with animal tumor models or patient samples. To explore the cell biology of cancer cells at high spatial and temporal resolution, the Core's in-house expertise enables the power of a superb suite of confocal microscopes to be accessible not only to cell biologists but also to the wider community of members of the Cancer Center. Imaging modalities for molecular detection available to the researchers at the MCCF are optical microscopes, including wide field (epifluorescence, bright field, polarizing and DIC), confocal (raster scanning, line scanning and spinning disc), time lapse microscopy, FLIM and digital scanners. Experiments involving uncaging experiments, FRAP and FRET, and Ratio imaging of calcium ions are also carried out at the MCCF. In addition to assistance and training on image acquisition, the MCCF staff provide assistance to researchers in image processing and analysis using Velocity, MetaMorph, Imaris and MatLab software. The broad range of services and collaborative work provided by the Molecular Cytology Core has supported the research of 120 investigators in the past year. During the past grant period the work of the Core has contributed to 315 publications of researchers from 5 research programs. For example, with the assistance of the Core, Studer and Tabar demonstrated that neural rosette cells represent the first characterized neural stem cell stage capable of responding to patterning cues that direct differentiation toward region-specific neuronal fates. The Core provided immunohistochemistry and image analysis critical to this work.

分子细胞学核心设施 (MCCF) 支撑着 MSKCC 的所有基础和临床研究涉及细胞、组织和肿瘤中蛋白质或核酸的研究。 MCCF 保持现状最先进的设备和一系列成像工具以及一支才华横溢的技术助理团队和这样既提供服务又提供一对一的培训，使教师及其学员能够工作独立于高端仪器仪表。用于自动或手动的经过验证的抗体资源组织学是动物肿瘤研究人员所使用的核心快速可靠服务的一个特点模型或患者样本。探索高时空癌细胞的细胞生物学分辨率，核心的内部专业知识使一套卓越的共焦显微镜的力量成为可能不仅细胞生物学家可以使用，癌症中心更广泛的成员社区也可以使用。 MCCF 研究人员可用的分子检测成像方式是光学成像方式显微镜，包括宽视野（落射荧光、明视野、偏光和 DIC）、共焦（光栅）扫描、线扫描和旋转盘）、延时显微镜、FLIM 和数字扫描仪。实验包括解笼实验、FRAP 和 FRET，以及钙离子比率成像也在 MCCF。除了图像采集方面的帮助和培训外，MCCF 工作人员还提供帮助研究人员使用 Velocity、MetaMorph、Imaris 和 MatLab 软件进行图像处理和分析。这分子细胞学核心提供的广泛服务和协作工作支持了去年120名研究者的研究。在过去的拨款期内，核心的工作已为 5 个研究项目的研究人员发表了 315 篇出版物。例如，在帮助下 Core、Studer 和 Tabar 的研究表明，神经花结细胞代表了第一个特征化的神经细胞。干细胞阶段能够响应模式线索，引导分化向区域特异性神经元的命运。 Core 提供了对这项工作至关重要的免疫组织化学和图像分析。