MOLECULAR CYTOLOGY

分子细胞学

基本信息

批准号：
8375206
负责人：
katia Ognianova manova
金额：
$ 25.11万
依托单位：
SLOAN-KETTERING INST CAN RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至 2013-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8375206
关键词：
Animals Apoptosis Area Bacteria Biological Biological Preservation Cancer Center Cancer Center Support Grant Cell Proliferation Cell physiology Cells Complement Consultations Core Facility Cultured Cells Cytology Detection Development Dissection Embryo Environment Evaluation Fixatives Human Hypoxia Image In Situ In Situ Hybridization Knock-out Lasers Life Malignant Neoplasms Memorial Sloan-Kettering Cancer Center Messenger RNA Methods Microscope Microscopy Molecular Mus Normal tissue morphology Optics Paraffin Phenotype Preparation Procedures Process Proteins RNA Research Research Personnel Sampling Scanning Services Staining method Stains Stem cells Techniques Technology Tissue Sample Tissues Training Transgenic Mice Tumor Tissue angiogenesis biological preparation experience laser capture microdissection mRNA Expression molecular marker protein expression reconstruction research study senescence tissue processing tumor

项目摘要

The main objective of the Molecular Cytology Core Facility (MCCF) is to provide to users state-of-the-art technologies in a multi-user environment, for preparation of tissue samples, for detection, precise localization and analysis of the expression of molecules with important cell functions during development and in cancer. The technologies include in situ localization of mRNA and proteins, cell proliferation, stem cell potential and differentiation, apoptosis and senescence, angiogenesis and hypoxia, histological evaluation and molecular in situ characterization of phenotypes of knock- out, transgenic mice and human tumors. In addition to the training and assistance provided to the researchers in the execution of these technologies, automated experiments for in situ molecular detection are carried at the MCCF. The optical microscopes [wide field, laser scanning (point scanning, spinning disc and multiphoton)] are efficiently used by researchers for image acquisition, including live imaging. Experienced MCCF staff is instrumental in assisting users with stereological analysis, co-localization studies and 3D reconstructions. Specific services provided are: tissue processing and sample preparation; execution of methods for in situ molecular detection; application of a wide range of optical microscopy techniques in conjunction with image acquisition and analysis.

分子细胞学核心设施（MCCF）的主要目标是为用户提供最先进多用户环境中的技术，用于制备组织样品，可检测，精确定位以及在发育和癌症过程中具有重要细胞功能的分子表达的分析。这些技术包括mRNA和蛋白质的原位定位，细胞增殖，干细胞电位和分化，凋亡和衰老，血管生成和缺氧，组织学评估和分子原位表征敲除，转基因小鼠和人类肿瘤的表型。除了在执行这些技术时，向研究人员提供的培训和帮助，自动化在MCCF处进行原位分子检测的实验。光学显微镜[宽场，研究人员有效地使用了激光扫描（点扫描，旋转盘和多光子）] 采集，包括现场成像。经验丰富的MCCF员工有助于帮助用户立体分析，共定位研究和3D重建。提供的特定服务是：组织加工和样品制备；原位执行方法分子检测；与图像结合使用多种光学显微镜技术的应用获取和分析。