INSULIN'S ACTION ON CAPILLARY RECRUITMENT

胰岛素对毛细血管复张的作用

• 批准号: 7718906
• 负责人: annaswamy raji
• 金额: $ 0.02万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7718906
• 关键词: Age; Asian Indian; Blood Vessels; Blood capillaries; Blood flow; Caucasians; Caucasoid Race; Computer Retrieval of Information on Scientific Projects Database; Dissociation; Dose; Euglycemic Clamping; Funding; Glucose Clamp; Goals; Grant; Human; Hyperinsulinism; Institution; Insulin; Insulin Resistance; Measures; Perfusion; Physiological; Research; Research Personnel; Resources; Skeletal Muscle; Source; Techniques; Ultrasonography; United States National Institutes of Health; brachial artery; capillary; glucose metabolism; glucose uptake; insulin sensitivity; non-diabetic

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This proposal focuses on insulin's action on both bulk and microvascular perfusion, and their relation to glucose metabolism in insulin resistant non-diabetic Asian Indians. The primary goal of this project is to assess if physiological levels of insulin enhance microvascular perfusion in human skeletal muscle by capillary recruitment, using the technique of contrast-enhanced ultrasound. We expect to see dissociation between insulin's action on capillary recruitment and total blood flow. Specifically, we hypothesize that insulin's action on capillary recruitment occurs earlier than changes in blood flow, and that there is a direct relationship between capillary recruitment and insulin's ability to stimulate glucose uptake in the skeletal muscle. Our second goal is to determine if healthy Asian Indians have blunted capillary recruitment that is related to their decreased insulin sensitivity. Healthy Asian Indian and Caucasians matched for BMI and age will undergo a hyperinsulinemic euglycemic clamp, and will have capillary recruitment and brachial artery blood flow measured at baseline, and after 30 and 120 minutes of hyperinsulinemia to assess the temporal relationship between vascular reactivity and glucose uptake. We anticipate that physiological doses of insulin will increase capillary recruitment and glucose uptake before its effect on increasing total blood flow occurs. We hypothesize that insulin resistant Asian Indians will have blunting of capillary recruitment, and that there will be a significant correlation between the magnitude of the blunting and the reduction in glucose uptake by the skeletal muscle compared to Caucasians.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该提案的重点是胰岛素对大血管和微血管灌注的作用，及其与胰岛素抵抗的非糖尿病亚洲印度人葡萄糖代谢的关系。该项目的主要目标是使用超声造影技术评估胰岛素的生理水平是否通过毛细血管募集来增强人体骨骼肌的微血管灌注。我们期望看到胰岛素对毛细血管募集的作用与总血流量之间的分离。具体来说，我们假设胰岛素对毛细血管募集的作用早于血流变化，并且毛细血管募集与胰岛素刺激骨骼肌葡萄糖摄取的能力之间存在直接关系。我们的第二个目标是确定健康的亚洲印度人是否因胰岛素敏感性降低而导致毛细血管募集减弱。 BMI 和年龄相匹配的健康亚洲印度人和白种人将接受高胰岛素正常血糖钳夹术，并在基线时测量毛细血管募集和肱动脉血流量，并在高胰岛素血症 30 和 120 分钟后测量，以评估血管反应性和葡萄糖摄取之间的时间关系。我们预计生理剂量的胰岛素会增加毛细血管的募集和葡萄糖的摄取，然后才会对增加总血流量产生影响。我们假设，胰岛素抵抗的亚洲印度人的毛细血管募集会变钝，并且与白种人相比，变钝的程度与骨骼肌葡萄糖摄取的减少之间存在显着相关性。