Transgenic Animal Genotyping and Phenotyping Core

转基因动物基因分型和表型核心

基本信息

批准号：
10558650
负责人：
Yumei Feng Earley
金额：
$ 30.95万
依托单位：
UNIVERSITY OF NEVADA RENO
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-01-01 至 2024-03-04
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10558650
关键词：
Abdomen Animal Model Animals Aorta Area Biosensor Blood Pressure Blood Pressure Monitors Blood Vessels Blood flow Breeding CRISPR/Cas technology Cardiac Cardiovascular Diseases Cardiovascular Physiology Cardiovascular system Cause of Death Centers of Research Excellence Collection Conscious Core Facility Database Management Systems Development Dimensions Disease model Echocardiography Equipment Funding Generations Genetic Genetically Modified Animals Genotype Goals Heart Rate Hypertension Infrastructure Injections Intervention Investigation Kidney Knock-out Knockout Mice Laboratories Laboratory Animals Laser-Doppler Flowmetry Logistics Maintenance Measurement Measures Methods Mission Modification Molecular Motor Activity Mus Nevada Phase Phenotype Protocols documentation Qualifying Rattus Research Research Personnel Research Project Grants Research Support Resources Services Signal Transduction Signal Transduction Pathway Solid Standardization Supervision Techniques Technology Telemetry Thick Training Transgenic Animals Transgenic Mice Transgenic Organisms Vendor Ventricular Function blood pressure regulation embryonic stem cell heart function hemodynamics in vivo mouse model novel novel therapeutics operation pharmacologic programs success tool ultrasound

项目摘要

Project Summary Genetically modified animals are fundamental for biomedical discovery, but breeding, genotyping, and maintenance of multiple transgenic lines is burdensome for junior investigators and their small laboratories. Further, most labs lack the specialized equipment and in vivo techniques needed to fully characterize the impact of genetic modification on essential cardiovascular functions. Therefore, the goal of the present proposal is to establish a new Transgenic Animal Genotyping and Phenotyping Research Core (Core B) as a centralized facility to provide these resources to support the research of the COBRE’s Project Leaders and the COBRE cardiovascular research group. The specific aims of Core B are to (1) establish and operate a centralized facility for maintaining genetically modified animals used for the investigation of molecular and cellular signal transduction pathways in the cardiovascular system; (2) establish and operate a facility that provides services for evaluating the cardiovascular phenotype of genetically modified animals; and (3) advise, train, and provide technical support for the use and characterization of transgenic laboratory animals. Briefly, Core B will supply the Project Leaders with access to a wide range of ready-to-use transgenic, knockout, and genetically encoded biosensor mice useful for the study of molecular and cellular signal transduction within the cardiovascular system. In addition, Core B will provide Project Leaders with a suite of in vivo techniques for characterization of the cardiovascular phenotypes of their animal models. These services include telemetric monitoring of blood pressure, heart rate, hemodynamics, and locomotor activity; echocardiography for evaluating cardiac function; and laser Doppler flowmetry for measuring blood flow. Successful completion of this proposal will alleviate the burden of maintaining and characterizing transgenic animal models. As such, Core B is vital for the rapid progress of the Project Leaders and their successful transition to independence. Furthermore, establishment of Core B in the greater Nevada area will provide a solid infrastructure and facility for the development of a highly competitive Cardiovascular Research group.

项目概要转基因动物是生物医学发现的基础，但育种、基因分型和维持多个转基因品系对于初级研究人员及其小型实验室来说是一项负担。此外，大多数实验室缺乏充分表征所需的专业设备和体内技术。因此，目前的目标是基因改造对基本心血管功能的影响。建议建立一个新的转基因动物基因分型和表型研究核心（核心B）作为集中设施提供这些资源来支持 COBRE 项目负责人和 COBRE 心血管研究小组的具体目标是 (1) 建立并运营一个维护用于分子和生物学研究的转基因动物的集中设施 (2) 建立并运营一个设施提供评估转基因动物心血管表型的服务；以及 (3) 建议，培训并为转基因实验动物的使用和表征提供技术支持。核心 B 将为项目负责人提供广泛的即用型转基因、基因敲除和基因编码的生物传感器小鼠可用于研究体内的分子和细胞信号转导此外，核心 B 将为项目负责人提供一套体内技术。这些服务包括遥测数据。监测血压、心率、血流动力学和超声心动图；评估心脏功能；以及用于测量血流量的激光多普勒流量计。该提案将减轻维护和表征转基因动物模型的负担。核心 B 对于项目负责人的快速进步及其成功过渡到独立至关重要。此外，在大内华达地区建立Core B将提供坚实的基础设施和设施发展一个具有高度竞争力的心血管研究小组。