Transgenic Animal Genotyping and Phenotyping Core

转基因动物基因分型和表型核心

• 批准号: 10558650
• 负责人: Yumei Feng Earley
• 金额: $ 30.95万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2024-03-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10558650
• 关键词: Abdomen; Animal Model; Animals; Aorta; Area; Biosensor; Blood Pressure; Blood Pressure Monitors; Blood Vessels; Blood flow; Breeding; CRISPR/Cas technology; Cardiac; Cardiovascular Diseases; Cardiovascular Physiology; Cardiovascular system; Cause of Death; Centers of Research Excellence; Collection; Conscious; Core Facility; Database Management Systems; Development; Dimensions; Disease model; Echocardiography; Equipment; Funding; Generations; Genetic; Genetically Modified Animals; Genotype; Goals; Heart Rate; Hypertension; Infrastructure; Injections; Intervention; Investigation; Kidney; Knock-out; Knockout Mice; Laboratories; Laboratory Animals; Laser-Doppler Flowmetry; Logistics; Maintenance; Measurement; Measures; Methods; Mission; Modification; Molecular; Motor Activity; Mus; Nevada; Phase; Phenotype; Protocols documentation; Qualifying; Rattus; Research; Research Personnel; Research Project Grants; Research Support; Resources; Services; Signal Transduction; Signal Transduction Pathway; Solid; Standardization; Supervision; Techniques; Technology; Telemetry; Thick; Training; Transgenic Animals; Transgenic Mice; Transgenic Organisms; Vendor; Ventricular Function; blood pressure regulation; embryonic stem cell; heart function; hemodynamics; in vivo; mouse model; novel; novel therapeutics; operation; pharmacologic; programs; success; tool; ultrasound

Project Summary Genetically modified animals are fundamental for biomedical discovery, but breeding, genotyping, and maintenance of multiple transgenic lines is burdensome for junior investigators and their small laboratories. Further, most labs lack the specialized equipment and in vivo techniques needed to fully characterize the impact of genetic modification on essential cardiovascular functions. Therefore, the goal of the present proposal is to establish a new Transgenic Animal Genotyping and Phenotyping Research Core (Core B) as a centralized facility to provide these resources to support the research of the COBRE’s Project Leaders and the COBRE cardiovascular research group. The specific aims of Core B are to (1) establish and operate a centralized facility for maintaining genetically modified animals used for the investigation of molecular and cellular signal transduction pathways in the cardiovascular system; (2) establish and operate a facility that provides services for evaluating the cardiovascular phenotype of genetically modified animals; and (3) advise, train, and provide technical support for the use and characterization of transgenic laboratory animals. Briefly, Core B will supply the Project Leaders with access to a wide range of ready-to-use transgenic, knockout, and genetically encoded biosensor mice useful for the study of molecular and cellular signal transduction within the cardiovascular system. In addition, Core B will provide Project Leaders with a suite of in vivo techniques for characterization of the cardiovascular phenotypes of their animal models. These services include telemetric monitoring of blood pressure, heart rate, hemodynamics, and locomotor activity; echocardiography for evaluating cardiac function; and laser Doppler flowmetry for measuring blood flow. Successful completion of this proposal will alleviate the burden of maintaining and characterizing transgenic animal models. As such, Core B is vital for the rapid progress of the Project Leaders and their successful transition to independence. Furthermore, establishment of Core B in the greater Nevada area will provide a solid infrastructure and facility for the development of a highly competitive Cardiovascular Research group.

项目摘要 转基因动物是生物医学发现的基础，但是育种，基因分型和 对于初级调查人员及其小型实验室而言，维持多个转基因线是繁重的。 此外，大多数实验室都缺乏充分表征的专业设备和体内技术 遗传修饰对必需心血管功能的影响。因此，目前的目标 建议是建立一种新的转基因动物基因分型和表型研究核心（核心B）作为一个 集中式设施提供这些资源，以支持COBRE项目负责人的研究 毛线心血管研究小组。核心B的具体目的是（1）建立和操作A 集中设施，用于维持用于研究分子和研究的一般修饰的动物 心血管系统中的细胞信号转移途径； （2）建立并运营一个设施 提供评估一般修饰动物的心血管表型的服务； （3）建议， 训练并为转基因实验室动物的使用和表征提供技术支持。简要地， 核心B将为项目负责人提供各种现成的转基因，淘汰和 遗传编码的生物传感器小鼠有助于研究分子和细胞信号转导内的生物传感器。 心血管系统。此外，核心B将为项目负责人提供一套体内技术 其动物模型的心血管表型的表征。这些服务包括遥测 监测血压，心率，血液动力学和运动活性；超声心动图 评估心脏功能；和激光多普勒流量指标，用于测量血流。成功完成 该建议将减轻维持和表征转基因动物模型的燃烧。像这样， 核心B对于项目领导者的快速进步及其成功过渡到独立至关重要。 此外，在大内华达州大区建立核心B将提供坚实的基础设施和设施 为了发展高度竞争性的心血管研究小组。