Mouse Phenotyping Research Core

小鼠表型研究核心

• 批准号: 8708142
• 负责人: Yumei Feng Earley
• 金额: $ 14.49万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8708142
• 关键词: Abdomen; Angiotensin II; Animals; Aorta; Area; Award; Biology; Blood Pressure; Blood Vessels; Blood pressure determination; Cardiac; Cardiovascular Diseases; Cardiovascular Physiology; Cardiovascular system; Centers of Research Excellence; Collaborations; Complement; Computers; Core Facility; Data Collection; Dimensions; Disease model; Eating; Echocardiography; Endothelium; Equipment; Excretory function; Filtration; Funding; Future; Generations; Glomerular Filtration Rate; Goals; Grant; Heart failure; Hour; Human Resources; Hypertension; Insulin-Like Growth Factor I; International; Kidney; Kidney Diseases; Logistics; Louisiana; Measurement; Metabolic; Methodology; Methods; Monitor; Mus; Muscular Atrophy; Nerve; Organ; Peripheral Nerves; Phenotype; Plants; Plethysmography; Renal Hypertension; Renal Plasma Flow; Renal clearance function; Renal function; Research; Research Personnel; Research Project Grants; Role; Running; Science; Sequoia; Signal Transduction; Sodium; Source; Splanchnic Nerves; Staging; Supervision; System; Techniques; Telemetry; Thick; Training; Transgenic Mice; Translational Research; Ultrasonography; United States National Institutes of Health; Universities; Urine; Vascular resistance; Ventricular Function; atherogenesis; base; endothelial dysfunction; experience; functional status; hemodynamics; investigator training; kidney vascular structure; macrophage; member; monocyte; mouse model; novel; operation; overexpression; pressure; receptor; relating to nervous system; research study; tool

For the past 9 years as a funded Center of Biomedical Research Excellence (COBRE), the THRCE has assembled an array of methodologies for assessing cardiac and renal function in mice based on the continuous hemodynamic and activity monitoring by telemetric transmitters, blood pressure determinations by plethysmography, single-mouse metabolic cage studies, renal functional experiments, renal nerve activity recording, and cardiac function by echocardiography. These measurement systems have been maintained and operated as a core facility within the THRCE-COBRE, and as such have become instrumental for the progress of much ongoing research at the THRCE. Indeed, these measurement systems are now being used by other researchers at the Tulane University and have led to a number of significant national and international collaborations. The goal of the present proposal is the continuance of this facility as a Mouse Phenotyping Research Core (MPRC) to allow it to provide a broader range of functions to biomedical researchers within the THRCE and the Tulane University. The MPRC will focus on the following specific aims: Specific Aim 1: To maintain and operate state-of-the-art methodologies for assessing arterial blood pressure, neural autonomic status and renal function in mouse models of hypertension and associated renal and cardiovascular diseases. Specific Aim 2: To maintain and operate a cardiovascular ultrasound system for characterizing mouse cardiac and vascular function in hypertension, renal and cardiovascular disease models. Specific Aim 3: To organize and train investigators in the usage of these facilities. The data collection capabilities offered by the MPRC are unique in the State of Louisiana. These capabilities are vital for the THRCE to maintain the research excellence necessary to transition its funding from the COBRE award to other competitive grants over the next 5 years and into the future.

在过去的 9 年里，THRCE 作为生物医学卓越研究中心 (COBRE) 的资助中心， 组装了一系列评估小鼠心脏和肾功能的方法 通过遥测发射器进行连续血流动力学和活动监测、血压测定 通过体积描记法、单鼠代谢笼研究、肾功能实验、肾神经活动 记录并通过超声心动图检查心脏功能。这些测量系统已得到维护 并作为 THRCE-COBRE 内的核心设施运行，因此已成为 THRCE 正在进行的许多研究的进展。事实上，这些测量系统现在正在被 杜兰大学的其他研究人员使用了该方法，并导致了许​​多重要的国家和 国际合作。本提案的目标是继续将该设施作为鼠标 表型研究核心 (MPRC) 使其能够为生物医学提供更广泛的功能 THRCE 和杜兰大学的研究人员。 MPRC将重点关注以下具体事项 目标： 具体目标 1：维持和运行最先进的动脉血压评估方法， 高血压及相关肾病小鼠模型的神经自主状态和肾功能 心血管疾病。 具体目标 2：维护和操作用于表征小鼠的心血管超声系统 高血压、肾脏和心血管疾病模型中的心脏和血管功能。 具体目标 3：组织和培训调查人员如何使用这些设施。 MPRC 提供的数据收集能力在路易斯安那州是独一无二的。这些能力 对于 THRCE 保持卓越研究至关重要，以将其资金从 COBRE 授予未来 5 年和未来其他竞争性资助。