Mouse Phenotyping Research Core

小鼠表型研究核心

基本信息

批准号：
8463748
负责人：
Yumei Feng Earley
金额：
$ 15.05万
依托单位：
TULANE UNIVERSITY OF LOUISIANA
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8463748
关键词：
Abdomen Angiotensin II Animals Aorta Area Award Biology Blood Pressure Blood Vessels Blood pressure determination Cardiac Cardiovascular Diseases Cardiovascular Physiology Cardiovascular system Centers of Research Excellence Collaborations Complement Computers Core Facility Data Collection Dimensions Disease model Eating Echocardiography Endothelium Equipment Excretory function Filtration Functional disorder Funding Future Generations Glomerular Filtration Rate Goals Grant Heart failure Hour Human Resources Hypertension Insulin-Like Growth Factor I International Kidney Kidney Diseases Logistics Louisiana Measurement Metabolic Methodology Methods Monitor Mus Muscular Atrophy Nerve Organ Peripheral Nerves Phenotype Plants Plethysmography Renal Hypertension Renal Plasma Flow Renal clearance function Renal function Research Research Personnel Research Project Grants Role Running Science Sequoia Signal Transduction Sodium Source Splanchnic Nerves Staging Supervision System Techniques Telemetry Thick Training Transgenic Mice Translational Research Ultrasonography United States National Institutes of Health Universities Urine Vascular resistance Ventricular Function atherogenesis base experience functional status hemodynamics investigator training kidney vascular structure macrophage member monocyte mouse model novel operation overexpression pressure receptor relating to nervous system research study tool

Abdomen Angiotensin II Animals Aorta Area Award Biology Blood Pressure Blood Vessels Blood pressure determination Cardiac Cardiovascular Diseases Cardiovascular Physiology Cardiovascular system Centers of Research Excellence Collaborations Complement Computers Core Facility Data Collection Dimensions Disease model Eating Echocardiography Endothelium Equipment Excretory function Filtration Functional disorder Funding Future Generations Glomerular Filtration Rate Goals Grant Heart failure Hour Human Resources Hypertension Insulin-Like Growth Factor I International Kidney Kidney Diseases Logistics Louisiana Measurement Metabolic Methodology Methods Monitor Mus Muscular Atrophy Nerve Organ Peripheral Nerves Phenotype Plants Plethysmography Renal Hypertension Renal Plasma Flow Renal clearance function Renal function Research Research Personnel Research Project Grants Role Running Science Sequoia Signal Transduction Sodium Source Splanchnic Nerves Staging Supervision System Techniques Telemetry Thick Training Transgenic Mice Translational Research Ultrasonography United States National Institutes of Health Universities Urine Vascular resistance Ventricular Function atherogenesis base experience functional status hemodynamics investigator training kidney vascular structure macrophage member monocyte mouse model novel operation overexpression pressure receptor relating to nervous system research study tool

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项目摘要

For the past 9 years as a funded Center of Biomedical Research Excellence (COBRE), the THRCE has assembled an array of methodologies for assessing cardiac and renal function in mice based on the continuous hemodynamic and activity monitoring by telemetric transmitters, blood pressure determinations by plethysmography, single-mouse metabolic cage studies, renal functional experiments, renal nerve activity recording, and cardiac function by echocardiography. These measurement systems have been maintained and operated as a core facility within the THRCE-COBRE, and as such have become instrumental for the progress of much ongoing research at the THRCE. Indeed, these measurement systems are now being used by other researchers at the Tulane University and have led to a number of significant national and international collaborations. The goal of the present proposal is the continuance of this facility as a Mouse Phenotyping Research Core (MPRC) to allow it to provide a broader range of functions to biomedical researchers within the THRCE and the Tulane University. The MPRC will focus on the following specific aims: Specific Aim 1: To maintain and operate state-of-the-art methodologies for assessing arterial blood pressure, neural autonomic status and renal function in mouse models of hypertension and associated renal and cardiovascular diseases. Specific Aim 2: To maintain and operate a cardiovascular ultrasound system for characterizing mouse cardiac and vascular function in hypertension, renal and cardiovascular disease models. Specific Aim 3: To organize and train investigators in the usage of these facilities. The data collection capabilities offered by the MPRC are unique in the State of Louisiana. These capabilities are vital for the THRCE to maintain the research excellence necessary to transition its funding from the COBRE award to other competitive grants over the next 5 years and into the future.

在过去的9年中，作为卓越生物医学研究中心（COBRE），THRCE拥有组装了一系列方法，用于评估小鼠的心脏和肾功能通过远程发射器，血压确定的连续血流动力学和活性监测通过多体积学，单小鼠代谢笼研究，肾功能实验，肾神经活动记录和通过超声心动图的心脏功能。这些测量系统已维护并作为Thrce-Cobre内的核心设施运行，因此已经对在THCE上进行了许多正在进行的研究的进展。确实，这些测量系统现在正在杜兰大学的其他研究人员使用，并导致了许多重要的国家和国际合作。本提案的目的是该设施作为鼠标的延续表型研究核心（MPRC）使其能够为生物医学提供更广泛的功能 THCE和杜兰大学的研究人员。 MPRC将专注于以下特定目标：特定目的1：维持和操作用于评估动脉血压的最新方法论，在高血压和相关肾脏的小鼠模型中，神经自主状态和肾功能心血管疾病。特定目标2：维护和操作心血管超声系统以表征鼠标高血压，肾脏和心血管疾病模型中的心脏和血管功能。特定目的3：组织和培训调查人员在这些设施的使用中。 MPRC提供的数据收集功能在路易斯安那州是独一无二的。这些功能对于TRECE保持卓越的研究至关重要，将其资金从在未来5年和未来，授予其他竞争赠款的手工奖。