Imaging Core

成像核心

• 批准号: 10259822
• 负责人: Indra Chandrasekar
• 金额: $ 13.15万
• 依托单位: SANFORD RESEARCH/USD
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10259822
• 关键词: Address; Area; Award; Bioinformatics; Biomedical Research; Cells; Centers of Research Excellence; Childhood; Computer software; Consult; Custom; Data; Data Analyses; Development; Disease; Ensure; Equipment; Fee-for-Service Plans; Fees; Fluorescence; Fluorescence Microscopy; Fluorescence Recovery After Photobleaching; Fluorescence Resonance Energy Transfer; Funding; Goals; Growth; Health; Histology; Human; Human Resources; Image; Image Analysis; Institution; Laboratory Research; Maintenance; Manuscripts; Methods; Microscope; Microscopy; Modernization; Pediatric Research; Phase; Photobleaching; Preparation; Protocols documentation; Research; Research Personnel; Research Support; Sampling; Science; Scientist; Secure; Services; Site; Structure; System; Systems Analysis; Techniques; Time; Training; Training Support; Translating; United States National Institutes of Health; base; design; experience; faculty research; fluorescence imaging; functional genomics; imaging capabilities; instrumentation; operation; screening; success

PROJECT SUMMARY The Imaging Core is an established entity within Sanford Research and provides integral technical during Phase I support of the Center for Pediatric Research in the maintenance and supports of microscopy-oriented imaging equipment and in the delivery of expertise in advanced microscopy techniques and analyses of imaging related data for Project Leaders and other Sanford Research faculty. The objective of the current application is to secure the continuation of Core's operations, to augment and enhance Core's capabilities, and to ensure the quality services of the Imaging Core to the new investigators within the Center for Pediatric Research, as well as continued Core support of the other Sanford Research investigators. To achieve this objective, the following specific aims are proposed: Aim 1: To expand the Imaging Core capabilities. We aim to expand our Core services to include support of a Thermo CellInsight CX7 High Content Screening system for rapid quantification of various cellular parameters, significantly reducing the amount of people-power/time needed for quantitative histology. We will add additional technical support (in conjunction with the Functional Genomics and Bioinformatics Core) that can provided analyst support. This expansion will meet the needs of the project leaders within the Center for Pediatric Disease, the other Sanford Research investigators, and scientists from regional institutions, including those at other NIH IdEA-funded sites, and will allow the Core to continue providing training, support and maintenance of its equipment. Aim 2: To provide assistance with project design, manuscript preparation and funding applications. The Core will directly interface with investigators during project design, protocol establishment, execution, and troubleshooting. The Core leads and manager will consult with investigators, offering an expertise in technical approaches. The proposed personnel expansion will enable us to deliver detailed experimental protocols used by the Core for manuscripts and funding applications. Aim 3: To create a sustainable Imaging Core. Our long-term goal is to establish a sustainable Core capable of fully supporting the research demands of our scientists. The Core will develop a fee-for-service structure, which, along with an institutional support, will sustain Core beyond the period of CoBRE support. The successful implementation of these proposed aims will translate into expanded and enhanced services by the Imaging Core, which will meet our growing technical demands. With the additional technical support and enhanced equipment, the Core will efficiently interact with the investigators during all stages of scientific projects, thus fulfilling its purpose: to support the goals of Core users, namely independent scientists seeking to uncover the mechanisms of human health and disease.

项目概要 成像核心是桑福德研究中心内的一个既定实体，并在阶段期间提供整体技术 我支持儿科研究中心维护和支持显微镜成像 设备以及提供先进显微镜技术和成像相关分析方面的专业知识 项目负责人和桑福德研究中心其他教员的数据。当前应用程序的目标是确保 核心业务的延续，增强和增强核心的能力，并确保质量 成像核心为儿科研究中心的新研究人员提供的服务，以及 其他桑福德研究中心研究人员的持续核心支持。为实现这一目标，应采取以下措施 提出了具体目标： 目标 1：扩展成像核心功能。我们的目标是扩展我们的核心服务，包括支持 Thermo CellInsight CX7 高内涵筛选系统可快速定量各种细胞 参数，显着减少定量组织学所需的人力/时间。我们将 添加额外的技术支持（与功能基因组学和生物信息学核心结合），可以 提供了分析师支持。此次扩建将满足儿科中心项目负责人的需求 疾病、桑福德研究中心的其他研究人员以及来自地区机构的科学家，包括 其他 NIH IdEA 资助的站点，并将允许核心继续提供培训、支持和维护 它的设备。 目标 2：为项目设计、稿件准备和资金申请提供帮助。 核心将在项目设计、方案建立、执行和实施过程中直接与研究者互动 故障排除。核心领导和经理将与调查人员协商，提供技术方面的专业知识 接近。拟议的人员扩充将使我们能够提供所使用的详细实验方案 由核心负责手稿和资助申请。 目标 3：创建可持续的成像核心。我们的长期目标是建立可持续发展的核心 能够充分支持我们科学家的研究需求。核心将制定按服务收费的方式 该结构与机构支持一起，将在 CoBRE 支持期结束后维持 Core。 这些拟议目标的成功实施将转化为扩大和增强的服务 通过成像核心，这将满足我们不断增长的技术需求。有了额外的技术支持和 增强的设备，核心将在科学项目的各个阶段与研究人员有效互动， 从而实现其目的：支持核心用户的目标，即寻求发现的独立科学家 人类健康和疾病的机制。