Imaging Core

成像核心

• 批准号: 9573146
• 负责人: Indra Chandrasekar
• 金额: $ 9.35万
• 依托单位: SANFORD RESEARCH/USD
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9573146
• 关键词: Address; Area; Award; Bioinformatics; Biomedical Research; Cells; Centers of Research Excellence; Childhood; Computer software; Consult; Custom; Data; Data Analyses; Development; Disease; Ensure; Equipment; Fee-for-Service Plans; Fees; Fluorescence; Fluorescence Microscopy; Fluorescence Recovery After Photobleaching; Fluorescence Resonance Energy Transfer; Funding; Goals; Growth; Health; Histology; Human; Human Resources; Image; Image Analysis; Institution; Laboratory Research; Maintenance; Manuscripts; Methods; Microscope; Microscopy; Modernization; Pediatric Research; Phase; Photobleaching; Preparation; Protocols documentation; Research; Research Personnel; Research Support; Sampling; Science; Scientist; Secure; Services; Site; Structure; System; Systems Analysis; Techniques; Time; Training; Training Support; Translating; United States National Institutes of Health; base; design; experience; faculty research; fluorescence imaging; functional genomics; imaging capabilities; instrumentation; operation; screening; success

PROJECT SUMMARY The Imaging Core is an established entity within Sanford Research and provides integral technical during Phase I support of the Center for Pediatric Research in the maintenance and supports of microscopy-oriented imaging equipment and in the delivery of expertise in advanced microscopy techniques and analyses of imaging related data for Project Leaders and other Sanford Research faculty. The objective of the current application is to secure the continuation of Core's operations, to augment and enhance Core's capabilities, and to ensure the quality services of the Imaging Core to the new investigators within the Center for Pediatric Research, as well as continued Core support of the other Sanford Research investigators. To achieve this objective, the following specific aims are proposed: Aim 1: To expand the Imaging Core capabilities. We aim to expand our Core services to include support of a Thermo CellInsight CX7 High Content Screening system for rapid quantification of various cellular parameters, significantly reducing the amount of people-power/time needed for quantitative histology. We will add additional technical support (in conjunction with the Functional Genomics and Bioinformatics Core) that can provided analyst support. This expansion will meet the needs of the project leaders within the Center for Pediatric Disease, the other Sanford Research investigators, and scientists from regional institutions, including those at other NIH IdEA-funded sites, and will allow the Core to continue providing training, support and maintenance of its equipment. Aim 2: To provide assistance with project design, manuscript preparation and funding applications. The Core will directly interface with investigators during project design, protocol establishment, execution, and troubleshooting. The Core leads and manager will consult with investigators, offering an expertise in technical approaches. The proposed personnel expansion will enable us to deliver detailed experimental protocols used by the Core for manuscripts and funding applications. Aim 3: To create a sustainable Imaging Core. Our long-term goal is to establish a sustainable Core capable of fully supporting the research demands of our scientists. The Core will develop a fee-for-service structure, which, along with an institutional support, will sustain Core beyond the period of CoBRE support. The successful implementation of these proposed aims will translate into expanded and enhanced services by the Imaging Core, which will meet our growing technical demands. With the additional technical support and enhanced equipment, the Core will efficiently interact with the investigators during all stages of scientific projects, thus fulfilling its purpose: to support the goals of Core users, namely independent scientists seeking to uncover the mechanisms of human health and disease.

项目摘要 成像核心是Sanford Research中的一个既定实体，并在阶段提供了不可或缺的技术 我支持小儿研究中心的维护和支持，以显微镜为导向的成像 设备和交付高级显微镜技术专业知识和与成像相关的分析 项目领导者和其他桑福德研究学院的数据。当前申请的目的是确保 核心运营的延续，增强和增强核心的能力，并确保质量 成像核心为儿科研究中心的新调查人员提供的服务以及 其他Sanford研究人员的核心支持持续支持。为了实现这一目标，以下 提出了具体目标： 目标1：扩展成像核心功能。我们旨在扩大我们的核心服务以包括支持 用于快速定量各种细胞的Thermo Cellinsight CX7高含量筛选系统 参数，大大减少了定量组织学所需的人力/时间/时间。我们将 添加额外的技术支持（与功能基因组学和生物信息学核心结合使用）可以 提供了分析师的支持。这种扩展将满足小儿中心项目领导者的需求 疾病，其他桑福德研究人员以及来自地区机构的科学家，包括 其他NIH Idea资助的网站，并将允许核心继续提供培训，支持和维护 它的设备。 目标2：为项目设计，手稿准备和资助应用提供帮助。 核心将在项目设计，协议建立，执行和 故障排除。核心领导和经理将咨询调查人员，提供技术专业知识 方法。拟议的人员扩展将使我们能够提供详细的实验协议 由手稿和资金申请的核心。 目标3：创建可持续成像核心。我们的长期目标是建立一个可持续的核心 能够充分支持我们科学家的研究要求。核心将开发费用为服务 结构将与机构支持一起，将维持核心的核心，超出山核桃的支撑时期。 这些提议的目标的成功实施将转化为扩展和增强的服务 通过成像核心，它将满足我们不断增长的技术需求。在额外的技术支持下 增强的设备，核心将在科学项目的所有阶段有效与研究人员相互作用， 因此实现了其目的：支持核心用户的目标，即寻求揭露的独立科学家 人类健康和疾病的机制。