IDENTIFICATION AND MOLECULAR BASIS FOR EFFICIENT ANTIFREEZE PROTEIN ENHANCERS

高效抗冻蛋白增强剂的鉴定和分子基础

基本信息

批准号：
10268213
负责人：
Xin Wen
金额：
$ 10.95万
依托单位：
CALIFORNIA STATE UNIVERSITY LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-30 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10268213
关键词：
Address Affect Affinity Alcohol dehydrogenase Alcohols Antifreeze Antifreeze Proteins Attention Bacteria Binding Biochemical Biological Biological Assay Biological Preservation Biophysics Body Fluids Bovine Serum Albumin Cells Cities Collaborations Conflict (Psychology)Creatine Kinase Cryopreservation Cryosurgery Crystallization Depressed mood Development Differential Scanning Calorimetry Enhancers Environment Enzymes Exhibits Fishes Freezing Frozen Foods Goals Growth Health High Pressure Liquid Chromatography Human Hydration status Hydrogen Bonding Hydrophobic Interactions Hydrophobicity Hyperactivity Ice Industrialization Insecta Lead Link Measures Methods Molecular Order Coleoptera Organ Organism Plants Proteins Reporting Research Rest Scientist Series Site-Directed Mutagenesis Sodium Chloride Structure Surface System Testing Time Tissues Translating Water Work base biological systems biophysical techniques carbonate dehydratase enzyme activity food quality fungus improved melting molecular mass novel novel strategies preservation protective effect protective efficacy small molecule solute

Address Affect Affinity Alcohol dehydrogenase Alcohols Antifreeze Antifreeze Proteins Attention Bacteria Binding Biochemical Biological Biological Assay Biological Preservation Biophysics Body Fluids Bovine Serum Albumin Cells Cities Collaborations Conflict (Psychology)Creatine Kinase Cryopreservation Cryosurgery Crystallization Depressed mood Development Differential Scanning Calorimetry Enhancers Environment Enzymes Exhibits Fishes Freezing Frozen Foods Goals Growth Health High Pressure Liquid Chromatography Human Hydration status Hydrogen Bonding Hydrophobic Interactions Hydrophobicity Hyperactivity Ice Industrialization Insecta Lead Link Measures Methods Molecular Order Coleoptera Organ Organism Plants Proteins Reporting Research Rest Scientist Series Site-Directed Mutagenesis Sodium Chloride Structure Surface System Testing Time Tissues Translating Water Work base biological systems biophysical techniques carbonate dehydratase enzyme activity food quality fungus improved melting molecular mass novel novel strategies preservation protective effect protective efficacy small molecule solute

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项目摘要

Abstract/Summary Antifreeze proteins (AFPs) are natural antifreeze molecules that have been found in many organisms including fish, insects, plants, bacteria, and fungi. AFPs exhibit great structural diversity, while they are all characterized by their unique ability to depress the freezing point of water without affecting the melting point apparently. The resulting difference between the melting point and the freezing point, referred to as thermal hysteresis (TH), is generally used as a measure of the antifreeze activities of AFPs. AFPs and co-solutes in body-fluids of cold-adapted organisms are responsible for survival in cold environments. Certain small molecule and protein co-solutes can further enhance the antifreeze activity of AFPs, referred to as enhancers, by binding to AFP through ionic interactions, hydrogen bonding, and/or hydrophobic interactions. AFP-based antifreeze systems (AFP plus enhancer) are much more effective and their uses are more environmentally friendly in comparison to conventional antifreezes, making them intriguing alternatives to conventional antifreezes in particular in biomedical fields. The proposed research will investigate the little known AFP- protein systems through determining the cryoprotective effect of an AFP on other proteins as well as the effect of these proteins on the antifreeze activity of the AFP using combined biochemical and biophysical methods. The information on such interplay in the AFP-protein systems would not only further our understanding of biological antifreeze system in cold-adapted organisms, but also lead to the development of effective protective systems for long-term preservation of important biologics in biomedical applications.

摘要/摘要抗冻蛋白（AFP）是在许多生物体中发现的天然抗冻分子包括鱼类，昆虫，植物，细菌和真菌。 AFP表现出巨大的结构多样性，而它们都是其特征是它们独特的能力降低水的冰点而不影响熔点显然。熔点和冰点之间的差异，称为热点滞后（Th）通常用作AFPS抗冻活动的量度。 AFPS和共同点冷适应生物体的体流体是在寒冷环境中生存的原因。某些小分子和蛋白质共糖可以进一步增强AFP的抗冻活性，称为增强子，通过通过离子相互作用，氢键和/或疏水相互作用与AFP结合。基于AFP 防冻系统（AFP Plus增强器）更加有效，其用途更加环境与传统的防冻剂相比，友好友好，使它们成为常规的替代品在生物医学领域，尤其是防冻子。拟议的研究将调查鲜为人知的AFP- 蛋白质系统通过确定AFP对其他蛋白质的冷冻保护作用以及影响这些蛋白质在AFP的抗冻活性上使用合并的生化和生物物理方法。有关AFP蛋白质系统中此类相互作用的信息不仅会进一步了解我们对冷适应生物的生物防冻系统，但也导致有效保护性的发展长期保存生物医学应用中重要生物制剂的系统。