New biomaterials for ocular surface reconstruction.

用于眼表重建的新型生物材料。

• 批准号: 7641481
• 负责人: Mark I Rosenblatt
• 金额: $ 25.35万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7641481
• 关键词: ABCG2 gene; Adherence; Adhesions; Adhesives; Animals; Behavior; Binding; Biocompatible Materials; Biological; Biological Assay; Biomedical Engineering; Cell Adhesion; Cell Adhesion Molecules; Cell Differentiation process; Cell Line; Cell surface; Cells; Characteristics; Chemicals; Cornea; Cytokeratin; Data; Debridement; Dental Inlays; Epithelial; Epithelial Cells; Epithelium; Evaluation; Eye; Fibronectins; Film; Fluorescence; Future; Growth; Healed; Human; Immigration; Immunohistochemistry; In Vitro; Laminin; Measures; Mechanics; Migration Assay; Modification; Molds; Monitor; Oryctolagus cuniculus; Pattern; Peptides; Play; Proliferating; Property; Protocols documentation; Resources; Role; Screening procedure; Silk; Stratification; Surface; Surface Properties; Surgical sutures; Tensile Strength; Tissues; Trauma; Vision; Wound Healing; base; body system; cell growth; cell motility; corneal epithelial cell adhesion; corneal epithelium; design; functional restoration; healing; heat injury; immortalized cell; imprint; in vivo; limbal; migration; nanofabrication; nanoscale; ocular surface; protein aminoacid sequence; public health relevance; reconstruction; regenerative; repaired; research study; scaffold; tyrosyl-isoleucyl-glycyl-seryl-arginine

DESCRIPTION (provided by applicant): The ocular surface is vulnerable to myriad insults including mechanical, chemical and thermal injuries. The resulting trauma may render the naturally occurring regenerative properties of the cornea incapable of restoring a healthy epithelial surface with a resultant loss of corneal transparency and vision. Bioengineered scaffolds derived from silk have been used to restore function to damaged tissues in number of organ systems. Although some progress has been made in ocular surface reconstruction, the emphasis has been on mechanisms to cultivate cells, while less focus has been placed on identifying means to alter the substrate on which the ocular surface epithelium is destined to grow. The proposal has 2 aims: 1) To determine the optimal nanoscale surface features promoting corneal epithelial cell adhesion and migration, and 2) To optimize cell adhesion and migration on silk films using peptide surface modifications. The above exploratory Aims will examine our hypothesis that nanoscale features and surface modifications are critical for the adhesion, proliferation and migration of corneal epithelial cells vivo. The Aims will lay the groundwork for future experiments combining modifications of ocular surface epithelial cell substrates using thin silk films as conduits for potential bioengineered ocular surface reconstruction. PUBLIC HEALTH RELEVANCE: The ocular surface is vulnerable to myriad insults including mechanical, chemical and thermal injuries. The resulting trauma may render the naturally occurring regenerative properties of the cornea incapable of restoring a healthy epithelial surface with a resultant loss of corneal transparency and vision. We propose to design bioengineered tissues composed of with surface properties that will facilitate repair of the eye.

描述（由申请人提供）：眼表容易受到多种损伤，包括机械损伤、化学损伤和热损伤。由此产生的创伤可能会使角膜自然发生的再生特性无法恢复健康的上皮表面，从而导致角膜透明度和视力丧失。由丝制成的生物工程支架已被用来恢复许多器官系统中受损组织的功能。尽管在眼表重建方面取得了一些进展，但重点一直放在培养细胞的机制上，而较少关注确定改变眼表上皮生长基质的方法。该提案有两个目标：1）确定促进角膜上皮细胞粘附和迁移的最佳纳米级表面特征，2）利用肽表面修饰优化丝膜上的细胞粘附和迁移。上述探索性目标将检验我们的假设，即纳米级特征和表面修饰对于体内角膜上皮细胞的粘附、增殖和迁移至关重要。该目标将为未来的实验奠定基础，利用丝薄膜作为潜在生物工程眼表重建的管道，结合眼表上皮细胞基质的修饰。公众健康相关性：眼表容易受到多种损伤，包括机械损伤、化学损伤和热损伤。由此产生的创伤可能会使角膜自然发生的再生特性无法恢复健康的上皮表面，从而导致角膜透明度和视力丧失。我们建议设计由具有促进眼睛修复的表面特性组成的生物工程组织。