Core B: Lipodomics Core

核心 B：脂质组学核心

• 批准号: 10247640
• 负责人: JOHN DOUGLAS HALEY
• 金额: $ 19.3万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10247640
• 关键词: Biochemistry; Biocompatible Materials; Bioinformatics; Biological; Biological Assay; Biology; Cancer Biology; Cell Culture Techniques; Cell model; Cells; Ceramides; Chemicals; Chemistry; Culture Media; Data; Data Analyses; Databases; Development; Educational workshop; Ensure; Enzymes; Evaluation Methodology; Experimental Designs; Family; Functional disorder; Future; Goals; High Pressure Liquid Chromatography; Home; Human Resources; Image; In Situ; In Vitro; Individual; International; Lead; Length; Lipid Chemistry; Lipids; Liquid substance; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Measures; Mentors; Metabolic; Metabolism; Methodology; Methods; Molecular; Molecular Analysis; Monitor; Neoplasm Metastasis; Pathway interactions; Pharmaceutical Preparations; Physiologic pulse; Plasma; Postdoctoral Fellow; Procedures; Program Research Project Grants; Proteins; Proteomics; Protocols documentation; Radioactive; Regulation; Research Personnel; Resource Sharing; Resources; Role; Serum; Services; Signal Transduction; Site; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sphingolipids; Students; Technology; Therapeutic; Tissues; Training; Update; Variant; Work; advanced analytics; analog; analytical method; analytical tool; base; biochemical tools; cancer therapy; computerized data processing; design; enzyme activity; experimental study; in vivo; inhibitor/antagonist; lipid mediator; lipid metabolism; lipidomics; metabolomics; programs; response; success; tandem mass spectrometry; tool; transcriptome sequencing; translational model; tumor metabolism; tumor progression; web site

Abstract The purpose of the Lipidomics Shared Resource Core (LSR Core) in the PPG is to provide intellectual and physical resources (particularly state-of-the-art mass spectrometry analysis of sphingolipids and flux analysis) to enhance our understanding of the role of bioactive lipids in cancer biology with the goal to discover future drugs. The diversity of bioactive lipids and their interconnected metabolism generates a network of pathways regulating intra- and inter-cellular signaling and function. Dysfunctions in these pathways contribute to the pathobiology of cancer progression and metastasis, the study of which is the main goal for the four experimental projects in this PPG. The vast diversity in sphingolipids with non-redundant, but often opposite functions, has resulted in a necessity for the development of new protocols and analytical methods for their identification and quantification. Appreciation for the depth of complexity involved in sphingolipid metabolism and signaling has recently expanded since (i) same species of sphingolipids could act differently depending on the subcellular compartment and (ii) variations in the acyl chain length or saturation status could lead to specific biological responses. These two new considerations exponentially increase the complexity on sphingolipid studies, and they have not been able to be interrogated until now. The LSR Core was created based on unique expertise of the key personnel in lipid chemistry, analysis, and metabolism, and it has evolved into a key Core for the program project grant (PPG) entitled Sphingolipids in Cancer Biology and Therapeutics. Furthermore, this Core has developed into an institutional, national, and international resource in the emerging fields of lipidomics and lipid chemical biology. Core services include: 1) qualitative and quantitative analysis of lipid components from different biological materials (cells, tissue, and biological fluids), primarily employing high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) technology—the Core currently provides quantitative analysis of more than 300 distinct lipid molecular species at a basic metabolomic level; 2) development of biochemical tools to study lipid metabolism (e.g., Sphingoproteomics, Sphingolipid Flux Analysis utilizing a Pulse-Chase of non-natural C17-sphingolipids, functionalized and fluorescent ceramides, site-specific radioactive sphingolipids); and 3) assisting PPG investigators in experimental design, selection of appropriate methods, and interpretation of analytical results. The LSR Core has been instrumental to the success of the PPG investigators over the past 16 years by providing them with unique capabilities and critical results that will continue to ensure the successful progress of this PPG.

抽象的 PPG 中脂质组学共享资源核心（LSR Core）的目的是提供 智力和物质资源（特别是最先进的质谱分析 鞘脂和通量分析）以增强我们对生物活性脂质在 癌症生物学，目标是发现未来的药物生物活性脂质及其多样性。 相互关联的代谢产生调节细胞内和细胞间的途径网络 这些途径的信号传导和功能障碍导致癌症的病理学。 进展和转移，其研究是四个实验项目的主要目标 在这种 PPG 中，鞘脂具有丰富的多样性，具有非冗余但通常相反的功能， 因此有必要为其开发新的协议和分析方法 认识鞘脂的复杂性。 新陈代谢和信号传导最近得到了扩展，因为（i）相同种类的鞘脂可以 根据亚细胞区室和 (ii) 酰基链长度的变化而发挥不同的作用 或饱和状态可能会导致特定的生物反应这两个新的考虑因素。 成倍增加鞘脂研究的复杂性，但他们还无法 LSR 核心是根据关键人员的独特专业知识创建的。 在脂质化学、分析和代谢领域，它已发展成为该计划的关键核心 此外，题为“癌症生物学和治疗中的鞘脂”的项目拨款（PPG）。 核心已发展成为新兴领域的机构、国家和国际资源 脂质组学和脂质化学生物学领域的核心服务包括：1）定性和 分析不同生物材料（细胞、组织）的脂质成分，并定量分析 生物体液），主要采用高效液相色谱-串联质谱 光谱分析 (HPLC-MS/MS) 技术——核心目前提供以下物质的定量分析： 基础代谢组学水平上超过 300 种不同的脂质分子种类；2) 研究脂质代谢的生化工具（例如鞘氨醇蛋白质组学、鞘脂通量分析） 利用非天然 C17-鞘脂、功能化和荧光脉冲追踪 神经酰胺、位点特异性放射性鞘脂）；以及 3) 协助 PPG 研究人员 实验设计、适当方法的选择以及分析结果的解释。 在过去 16 年里，LSR Core 对 PPG 调查人员的成功发挥了重要作用 多年来，为他们提供独特的能力和关键成果，将继续确保 此次PPG的顺利进展。