NIGMS National and Regional Resources - DNASU

NIGMS 国家和地区资源 - DNASU

• 批准号: 10238934
• 负责人: JOSHUA LABAER
• 金额: $ 86.35万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-15 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10238934
• 关键词: 11 year old; Academia; Animal Model; Archives; Bacteria; Bar Codes; Bioinformatics; Biological; Biological Process; Biomedical Research; Cells; Cloning; Collection; Communities; Complementary DNA; Computer software; Cultured Cells; Custom; DNA; DNA Sequence; Data; Databases; Deposition; Development; Disease; Ensure; Equipment; Explosion; Expression Library; Fees; Fees and Charges; Functional disorder; Funding; Gene Transfer; Genes; Genetic; Genetic Recombination; Genome; Glycerol; Government; Growth; Heart; Human; Human Resources; In Vitro; Individual; Industry; Informatics; Infrastructure; Insecta; Investments; Knowledge; Length; Libraries; Light; Link; Methods; Minority; Modernization; National Institute of General Medical Sciences; Ontology; Open Reading Frames; Operating System; Organism; Orphan; Pathway interactions; Plant Roots; Plasmids; Price; Process; Production; Protein Structure Initiative; Proteins; Proteome; Protocols documentation; Publications; Reading Frames; Recovery; Research; Research Personnel; Resource Sharing; Resources; Sales; Scientist; Services; Ships; Side; Site; Software Tools; System; Time; Update; Vertebral column; Visit; analytical tool; base; bioinformatics pipeline; computer infrastructure; computer program; cost; data handling; design; disease diagnosis; e-commerce; experience; expression cloning; expression vector; flexibility; genome sequencing; human disease; improved; interest; member; next generation sequencing; operation; outreach; potential biomarker; programs; protein expression; protein function; repository; social media; software development; software systems; targeted biomarker; tool; tool development; user-friendly; vector; virtual; web site

PROJECT SUMMARY DNASU is a 11 year old non-profit, academic-based repository that provides academia, industry and the government with a wide variety of plasmids including specialized vectors, regulatory genetic sequences, and genes from 1,269 organisms, with many complete genome collections, including the first complete human set. Users access the repository through our website, https://dnasu.org/, where they can: view pertinent clone, gene and vector information; order clones; and utilize link-outs to reach external resources such as protocols and publications. DNASU acquires plasmids both through in-house production by our high throughput cloning pipeline and through deposition by outside research groups. In addition, we partnered with the Protein Structure Initiative (PSI), for whom we performed full-length sequence verification and annotation on plasmids from multiple PSI centers before distributing them. Distribution is initiated when a user visits DNASU to request selected construct(s). Technicians subculture and ship the glycerol stocks for a nominal re-charge fee. DNASU maintains and archives over 260,000 unique plasmids. The DNASU website sees an average of 3,000 unique visitors per month, receives 5,000 orders and distributes over 35,000 plasmids per year to scientists worldwide. DNASU received funding from NIGMS through the PSI initiative that enabled the addition of >93,000 plasmids to its collection and the development of software tools for locating and using clones, thus creating a unique worldwide resource. We also received funds from NIGMS Legacy program to improve the infrastructure, member collection, visibility and sustainability of the repository. We have made significant inroads to create a self-sustaining effort whereby we can continue to distribute clones from our existing collection. However, the number of clones we distribute, although large, is small relative to the massive size of the collection we must maintain, so our sales do not support continued database/computer programming, upkeep and updating of our collection, or acquisition of new collections. We recognize that the scientific community needs a comprehensive archival site like ours in order to continue expanding scientific knowledge on the many proteins that have not yet been studied. We are seeking funding to support our operations and customer support to: 1) Maintain and Improve the Physical Plasmid Repository by refining wet lab methods, updating targeted collections to current expression vectors, refreshing clones to ensure viability, and assisting with acquisition of orphaned collections; 2) Enhance Bioinformatics Capabilities of DNASU to stay current with modern hardware, software and operating systems, and to enhance the DNASU database and bioinformatics pipelines for sequence annotation of the physical clones and the related biological information; and 3) Improve Customer Experience and Outreach by re-constructing the DNASU interface for order placement, improving technical support, and engaging scientists through social media support.

项目摘要 DNASU是一家11岁的非营利性，基于学术的存储库，可提供学术界，行业和 具有各种质粒的政府，包括专业媒介，监管遗传序列和 来自1,269种生物的基因，其中包括许多完整的基因组收集，包括第一个完整的人集。 用户可以通过我们的网站https://dnasu.org/访问存储库，在那里他们可以：查看相关的克隆， 基因和矢量信息；订购克隆；并利用链接到达外部资源，例如协议 和出版物。 DNASU通过我们的高吞吐量克隆通过内部生产获得质粒 管道以及外部研究小组的沉积。此外，我们与蛋白质合作 结构倡议（PSI），我们对质粒进行全长序列验证和注释 在分发之前从多个PSI中心。当用户访问DNASU要求请求时，发行发行 选定的构造。技术人员亚文化并将甘油库存运送，以象征性的重新收费。 dnasu 维护和档案超过260,000个独特的质粒。 DNASU网站平均看到3,000个独特 每月游客每年接收5,000个订单，并每年向全球科学家分发35,000多种质粒。 DNASU通过PSI倡议获得了Nigms的资金，该计划使添加> 93,000 其收集的质粒及其用于定位和使用克隆的软件工具的开发，从而创建一个 独特的全球资源。我们还收到了Nigms Legacy计划的资金，以改善 存储库的基础设施，会员收集，可见性和可持续性。我们已经很重要 涉足创造自我维持的努力，我们可以继续从现有的 收藏。但是，我们分发的克隆数量虽然很大，但相对于大小的大小很小 我们必须维护的集合，因此我们的销售不支持继续数据库/计算机编程， 维护和更新我们的收藏或收购新收藏。我们认识到科学 社区需要像我们这样的全面档案网站，以便继续扩展科学知识 在尚未研究的许多蛋白质上。我们正在寻求资金来支持我们的业务， 客户支持：1）通过完善湿实验室来维护和改善物理质粒存储库 方法，将有针对性的集合更新为当前的表达向量，刷新克隆以确保可行性以及 协助收购孤儿收藏； 2）增强DNASU的生物信息学能力 具有现代硬件，软件和操作系统的最新目前，并增强DNASU数据库和 物理克隆序列注释和相关生物学信息的生物信息学管道； 3）通过重新构建DNASU接口以改善客户体验和外展 通过社交媒体支持，安置，改善技术支持并吸引科学家。