NIGMS National and Regional Resources - DNASU

NIGMS 国家和地区资源 - DNASU

基本信息

批准号：
10025623
负责人：
JOSHUA LABAER
金额：
$ 124.57万
依托单位：
ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-15 至 2025-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10025623
关键词：
11 year old Academia Animal Model Archives Bacteria Bar Codes Bioinformatics Biological Biological Process Biomedical Research Cells Cloning Collection Communities Complementary DNA Computer software Cultured Cells Custom DNA DNA Sequence Data Databases Deposition Development Disease Ensure Equipment Explosion Expression Library Fees Fees and Charges Functional disorder Funding Gene Transfer Genes Genetic Genetic Recombination Genome Glycerol Government Growth Heart Human Human Resources In Vitro Individual Industry Informatics Infrastructure Insecta Investments Knowledge Length Libraries Light Link Methods Minority Modernization National Institute of General Medical Sciences Ontology Open Reading Frames Operating System Organism Orphan Pathway interactions Plant Roots Plasmids Price Process Production Protein Structure Initiative Proteins Proteome Protocols documentation Publications Reading Frames Recovery Research Research Personnel Resource Sharing Resources Sales Scientist Services Ships Side Site Software Tools System Time Update Vertebral column Visit analytical tool base bioinformatics pipeline computer infrastructure computer program cost design disease diagnosis e-commerce experience expression cloning expression vector flexibility genome sequencing human disease improved interest member next generation sequencing operation outreach potential biomarker programs protein expression protein function repository social media software development software systems targeted biomarker tool tool development user-friendly vector virtual web site

项目摘要

PROJECT SUMMARY DNASU is a 11 year old non-profit, academic-based repository that provides academia, industry and the government with a wide variety of plasmids including specialized vectors, regulatory genetic sequences, and genes from 1,269 organisms, with many complete genome collections, including the first complete human set. Users access the repository through our website, https://dnasu.org/, where they can: view pertinent clone, gene and vector information; order clones; and utilize link-outs to reach external resources such as protocols and publications. DNASU acquires plasmids both through in-house production by our high throughput cloning pipeline and through deposition by outside research groups. In addition, we partnered with the Protein Structure Initiative (PSI), for whom we performed full-length sequence verification and annotation on plasmids from multiple PSI centers before distributing them. Distribution is initiated when a user visits DNASU to request selected construct(s). Technicians subculture and ship the glycerol stocks for a nominal re-charge fee. DNASU maintains and archives over 260,000 unique plasmids. The DNASU website sees an average of 3,000 unique visitors per month, receives 5,000 orders and distributes over 35,000 plasmids per year to scientists worldwide. DNASU received funding from NIGMS through the PSI initiative that enabled the addition of >93,000 plasmids to its collection and the development of software tools for locating and using clones, thus creating a unique worldwide resource. We also received funds from NIGMS Legacy program to improve the infrastructure, member collection, visibility and sustainability of the repository. We have made significant inroads to create a self-sustaining effort whereby we can continue to distribute clones from our existing collection. However, the number of clones we distribute, although large, is small relative to the massive size of the collection we must maintain, so our sales do not support continued database/computer programming, upkeep and updating of our collection, or acquisition of new collections. We recognize that the scientific community needs a comprehensive archival site like ours in order to continue expanding scientific knowledge on the many proteins that have not yet been studied. We are seeking funding to support our operations and customer support to: 1) Maintain and Improve the Physical Plasmid Repository by refining wet lab methods, updating targeted collections to current expression vectors, refreshing clones to ensure viability, and assisting with acquisition of orphaned collections; 2) Enhance Bioinformatics Capabilities of DNASU to stay current with modern hardware, software and operating systems, and to enhance the DNASU database and bioinformatics pipelines for sequence annotation of the physical clones and the related biological information; and 3) Improve Customer Experience and Outreach by re-constructing the DNASU interface for order placement, improving technical support, and engaging scientists through social media support.

项目概要 DNASU 是一个已有 11 年历史的非营利性学术存储库，为学术界、工业界和政府拥有多种质粒，包括专用载体、调控基因序列和来自 1,269 个生物体的基因，拥有许多完整的基因组集合，包括第一个完整的人类基因组。用户通过我们的网站 https://dnasu.org/ 访问存储库，他们可以：查看相关克隆，基因和载体信息；订购克隆；并利用链接输出来访问协议等外部资源和出版物。 DNASU 通过我们的高通量克隆通过内部生产获得质粒管道并通过外部研究小组的沉积。此外，我们还与 Protein Structure Initiative (PSI)，我们为他们进行了质粒全长序列验证和注释在分发之前从多个 PSI 中心获取。当用户访问 DNASU 请求时启动分发选定的构建体。技术人员以象征性的充值费进行继代培养和运输甘油库存。 DNASU 维护并存档超过 260,000 个独特的质粒。 DNASU 网站平均有 3,000 个独特的每月接待访客 5,000 个订单，每年向世界各地的科学家分发超过 35,000 个质粒。 DNASU 通过 PSI 计划获得了 NIGMS 的资助，增加了超过 93,000 名收集质粒并开发用于定位和使用克隆的软件工具，从而创建独特的全球资源。我们还收到了 NIGMS Legacy 计划的资金，以改善存储库的基础设施、成员集合、可见性和可持续性。我们已经做出了重大的努力创造一种自我维持的努力，使我们能够继续从现有的产品中分发克隆收藏。然而，我们分发的克隆数量虽然很大，但相对于我们必须维护的集合，因此我们的销售不支持持续的数据库/计算机编程，维护和更新我们的藏品，或收购新的藏品。我们认识到科学社区需要像我们这样的综合档案网站，以便继续扩展科学知识许多尚未被研究的蛋白质。我们正在寻求资金来支持我们的运营和客户支持：1) 通过改进湿实验室维护和改进物理质粒存储库方法，将目标集合更新为当前表达载体，刷新克隆以确保活力，以及协助收购孤儿藏品； 2) 增强 DNASU 的生物信息学能力配备最新的现代硬件、软件和操作系统，并增强 DNASU 数据库和用于物理克隆和相关生物信息的序列注释的生物信息学管道； 3) 通过重新构建 DNASU 订单界面来改善客户体验和外展安置、改善技术支持以及通过社交媒体支持吸引科学家。