FEI Tecnai G2 Spirit BioTWIN Transmission Electron Microscope

FEI Tecnai G2 Spirit BioTWIN 透射电子显微镜

• 批准号: 7795560
• 负责人: MARILYN Gist FARQUHAR
• 金额: $ 49.97万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-28 至 2011-01-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795560
• 关键词: 20 year old; Age; Agreement; Arts; Biological; Biology; Cells; Communicable Diseases; Cryoultramicrotomy; Developmental Biology; Discipline; Electron Microscope; Electron Microscopy Facility; Ensure; Environment; Funding; Future; Image; Immunology; Label; Maintenance; Membrane Protein Traffic; Microscopy; Molecular Medicine; Molecular and Cellular Biology; Neurosciences; Organism; Organogenesis; Pathology; Plague; Plastics; Recovery; Research; Research Personnel; Resource Sharing; Sampling; Signal Transduction; Staining method; Stains; System; Techniques; Time; Transmission Electron Microscopy; United States National Institutes of Health; Vascular remodeling; Wages; Work; cost; instrument; lens; medical schools; meetings; partial recovery; transmission process

DESCRIPTION (provided by applicant): A group of 27 NIH funded investigators at UCSD requests funds to purchase a FEI Tecnai G2 Spirit BioTWIN transmission electron microscope (TEM) to continue to meet the ongoing and future demands of a variety of researchers within the Department of Cellular and Molecular Medicine (CMM), within the School of Medicine, and within the Division of Biology at UCSD. The requested TEM has an objective lens configuration optimized for high contrast microscopy of biological samples and is perfectly suited for imaging conventional ultrathin plastic sections, immunogold labeled ultrathin cryosections, and negatively stained samples. The Tecnai system is particularly adaptable to a diverse group of users and a multi-user environment. The Department's current 20 year old instruments are showing clear signs of wear and are plagued by increasing down time due to the age and past usage of the instruments. Future reliability is a major concern, and we are seeking to replace one of the TEMs with a modern, state-of-the-art instrument. Our NIH funded investigators study a variety of projects, ranging from single cell organisms to membrane trafficking, and signaling during vascular remodeling, organogenesis and host bacterial interactions. Their work spans a wide variety of disciplines from cell and molecular biology to immunology, pathology, neuroscience, developmental biology and infectious disease. All have in common the fact that they center on cell biological questions that require ultrastructural analysis and immunolocalization studies. The new TEM will ensure that our group of outstanding investigators have continued and uninterrupted access to the techniques and applications necessary for state-of-the-art research in these fields. The FEI Tecnai G2 Spirit BioTWIN will be located, administered and maintained in the existing CMM Transmission Electron Microscopy Facility -- a shared resource that is utilized regularly by numerous researchers. The new TEM will be included in the existing cost recovery system to ensure partial recovery of funds for salaries, maintenance agreements and supplies.

描述（由申请人提供）：一组NIH资助的UCSD的研究人员要求资金购买Fei Tecnai G2 Spirit Biotwin传播电子显微镜（TEM），以继续满足医学和医学学院内部以及在UCS内部的医学学院内部以及UCS内部的各种研究人员的各种研究人员的持续和未来需求。请求的TEM具有针对生物样品的高对比度显微镜进行优化的客观晶状体构型，非常适合成像传统的超薄塑料截面，标记为超薄的冷冻切除术和染色的样品。 Tecnai系统特别适应多种用户和多用户环境。该部门目前的20年仪器显示出明显的磨损迹象，并因仪器的年龄和过去使用而增加了停机时间。未来的可靠性是一个主要问题，我们正在寻求用现代，最先进的工具代替其中一个TEM。 我们的NIH资助的研究人员研究了各种项目，从单细胞生物到膜运输，以及血管重塑，器官发生和宿主细菌相互作用期间的信号传导。他们的工作涵盖了从细胞和分子生物学到免疫学，病理学，神经科学，发育生物学和传染病的各种学科。所有这些都有一个事实，即它们以需要超微结构分析和免疫定位研究的细胞生物学问题为中心。新的TEM将确保我们的杰出调查人员持续且不间断地访问这些领域最新研究所需的技术和应用程序。 Fei Tecnai G2 Spirit Biotwin将在现有的CMM传输电子显微镜设施中找到，管理和维护 - 这是一种共享资源，该资源经常被许多研究人员使用。新的TEM将包括在现有的成本回收系统中，以确保部分收回薪金，维护协议和物资的资金。