FEI Tecnai G2 Spirit BioTWIN Transmission Electron Microscope

FEI Tecnai G2 Spirit BioTWIN 透射电子显微镜

• 批准号: 7795560
• 负责人: MARILYN Gist FARQUHAR
• 金额: $ 49.97万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-28 至 2011-01-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795560
• 关键词: 20 year old; Age; Agreement; Arts; Biological; Biology; Cells; Communicable Diseases; Cryoultramicrotomy; Developmental Biology; Discipline; Electron Microscope; Electron Microscopy Facility; Ensure; Environment; Funding; Future; Image; Immunology; Label; Maintenance; Membrane Protein Traffic; Microscopy; Molecular Medicine; Molecular and Cellular Biology; Neurosciences; Organism; Organogenesis; Pathology; Plague; Plastics; Recovery; Research; Research Personnel; Resource Sharing; Sampling; Signal Transduction; Staining method; Stains; System; Techniques; Time; Transmission Electron Microscopy; United States National Institutes of Health; Vascular remodeling; Wages; Work; cost; instrument; lens; medical schools; meetings; partial recovery; transmission process

DESCRIPTION (provided by applicant): A group of 27 NIH funded investigators at UCSD requests funds to purchase a FEI Tecnai G2 Spirit BioTWIN transmission electron microscope (TEM) to continue to meet the ongoing and future demands of a variety of researchers within the Department of Cellular and Molecular Medicine (CMM), within the School of Medicine, and within the Division of Biology at UCSD. The requested TEM has an objective lens configuration optimized for high contrast microscopy of biological samples and is perfectly suited for imaging conventional ultrathin plastic sections, immunogold labeled ultrathin cryosections, and negatively stained samples. The Tecnai system is particularly adaptable to a diverse group of users and a multi-user environment. The Department's current 20 year old instruments are showing clear signs of wear and are plagued by increasing down time due to the age and past usage of the instruments. Future reliability is a major concern, and we are seeking to replace one of the TEMs with a modern, state-of-the-art instrument. Our NIH funded investigators study a variety of projects, ranging from single cell organisms to membrane trafficking, and signaling during vascular remodeling, organogenesis and host bacterial interactions. Their work spans a wide variety of disciplines from cell and molecular biology to immunology, pathology, neuroscience, developmental biology and infectious disease. All have in common the fact that they center on cell biological questions that require ultrastructural analysis and immunolocalization studies. The new TEM will ensure that our group of outstanding investigators have continued and uninterrupted access to the techniques and applications necessary for state-of-the-art research in these fields. The FEI Tecnai G2 Spirit BioTWIN will be located, administered and maintained in the existing CMM Transmission Electron Microscopy Facility -- a shared resource that is utilized regularly by numerous researchers. The new TEM will be included in the existing cost recovery system to ensure partial recovery of funds for salaries, maintenance agreements and supplies.

描述（由申请人提供）： UCSD 的 27 名 NIH 资助研究人员请求资金购买 FEI Tecnai G2 Spirit BioTWIN 透射电子显微镜 (TEM)，以继续满足该部门内各种研究人员当前和未来的需求细胞和分子医学 (CMM)，隶属于加州大学圣地亚哥分校医学院和生物学系。所需的 TEM 具有针对生物样品的高对比度显微镜优化的物镜配置，非常适合对传统超薄塑料切片、免疫金标记超薄冷冻切片和负染色样品进行成像。 Tecnai 系统特别适合不同的用户组和多用户环境。该部门目前已有 20 年历史的仪器已显示出明显的磨损迹象，并且由于仪器的使用年限和过去的使用情况而导致停机时间增加。未来的可靠性是一个主要问题，我们正在寻求用现代、最先进的仪器取代其中一台 TEM。 我们 NIH 资助的研究人员研究了各种项目，从单细胞生物到膜运输，以及血管重塑、器官发生和宿主细菌相互作用期间的信号传导。他们的工作涵盖从细胞和分子生物学到免疫学、病理学、神经科学、发育生物学和传染病等多个学科。所有这些都有一个共同点，即它们都以需要超微结构分析和免疫定位研究的细胞生物学问题为中心。新的 TEM 将确保我们的杰出研究人员团队能够持续不间断地获取这些领域最先进研究所需的技术和应用。 FEI Tecnai G2 Spirit BioTWIN 将在现有的 CMM 透射电子显微镜设施中进行定位、管理和维护，这是众多研究人员定期使用的共享资源。新的TEM将纳入现有的成本回收系统，以确保部分回收工资、维护协议和用品资金。