UAB Multi-user Fermentation Facility Upgrade

UAB 多用户发酵设施升级

• 批准号: 7795575
• 负责人: NORMAN P. HIGGINS
• 金额: $ 50万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-10 至 2011-06-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795575
• 关键词: Bacteria; Biochemical; Biochemistry; Cells; Charge; Complex; Deubiquitination; Disease; Engineering; Enzymes; Equipment; Faculty; Fermentation; Funding; Future; Goals; Grant; Growth; Harvest; Health; Hela Cells; Housing; Human Resources; Knowledge; Laboratories; Lipids; Locales; Maintenance; Molecular; Nature; New Brunswick; Nucleic Acids; Organism; Patient Care; Persons; Polysaccharides; Recruitment Activity; Request for Applications; Research; Research Personnel; Running; Schedule; Science; Services; Site; Steam; Structure; Time; United States National Institutes of Health; Universities; Visit; Wages; Water; Wisconsin; Work; Yeasts; cost; design; effective therapy; human disease; instrument; medical schools; medical specialties; operation; public health relevance; square foot; tissue/cell culture; tool

DESCRIPTION (provided by applicant): The Department of Biochemistry Fermentation Facility opened in 1984 with new equipment purchased from an NIH (S10) Shared Instrument Grant. The original building was built to serve the needs of a rapidly growing medical school with Biochemistry Department led by Robert D. Wells. Dr. Wells was recruited to Birmingham from the University of Wisconsin to add 10 new faculties focused on molecular studies of a range of biochemical specialties. The needs of this group included isolating enzymes, lipids, nucleic acids, and polysaccharides from a range of cultured organisms including bacteria, yeast, and tissue culture cells. One full time operating manager, Woody Robbins, hired in 1984, and part time technicians helped UAB and visiting scholars use the instruments for their specific customized experimental purposes. In 1998, the Fermentation facility moved from its original site to the current locale at the edge of the UAB campus. The structure is a standalone building that houses a cluster of laboratories specifically designed with special water, steam, and electrical provisions for large scale experimental growth chambers and fermentation equipment. The Fermentation facility occupies 2000 sq ft in this building. Dr. N. Patrick Higgins now directs the Fermentation Lab, which operates with two full time personnel. Woody Robbins is the Engineering Manager of the unit, and has served in that capacity for 25 years. He runs the operation and maintenance schedule and is the primary contact person for users inside and outside the university. Kim Hardy has been a key Research Assistant for 12 years. Kim took charge of growing 2000 L of HeLa cells that allowed Hengbin Wang to isolate a deubiquitination complex (see Joo, H. Y., L. Zhai, et al. (2007). Nature 449(7165): 1068-72. The lab runs on a break even strategy to recover running costs and salary for the two essential workers. This has worked well since 2001. The existing fermentation equipment consists of five fermentors made by New Brunswick Scientific. Two units are not operational and it is becoming increasingly difficult to obtain spare parts for the remaining units. This application requests funding for the purchase of 3 new fermentors and a cell-harvesting centrifuge to replace the old units so that the facility can continue well into the future providing investigators with the tools to efficiently conduct research in health related sciences. PUBLIC HEALTH RELEVANCE: The investigators taking advantage of the UAB Fermentation Facility's services are conducting basic health related research to better understand a variety of human diseases. The goal of this research is to gain a thorough understanding of the underlying mechanisms of specific diseases and applying that knowledge to developing more effective treatments and patient care.

描述（由申请人提供）：生物化学发酵设施于1984年开业，并从NIH（S10）共享工具赠款购买了新设备。原始建筑物的建造旨在满足生物化学快速增长的医学院的需求 罗伯特·D·威尔斯（Robert D. Wells）领导的部门。威尔斯博士从威斯康星大学招募到伯明翰，增加了10个专注于一系列生化专业的分子研究的新系。该组的需求包括分离酶，脂质，核酸和多糖，包括细菌，酵母和组织培养细胞，包括细菌，酵母和组织培养细胞。一位全职操作经理伍迪·罗宾斯（Woody Robbins）于1984年聘请，兼职技术人员帮助UAB和访问学者使用这些工具用于其特定的定制实验目的。 1998年，发酵设施从其原始地点转移到了UAB校园边缘的当前场所。该结构是一栋独立的建筑物，该建筑物容纳了专门设计的一组实验室，该实验室专门为大规模的实验生长室和发酵设备设计而设计。发酵设施在这座建筑物中占据2000平方英尺。 N. Patrick Higgins博士现在指挥发酵实验室，该实验室与两名全职人员一起运作。伍迪·罗宾斯（Woody Robbins）是该部门的工程经理，并以这种身份服务了25年。他运行操作和维护时间表，是大学内外用户的主要联系人。金·哈迪（Kim Hardy）担任关键研究助理已有12年了。 Kim负责增长2000升HELA细胞，使Hengbin Wang能够分离出去泛素化的络合物（请参阅Joo，H。Y.，L。Zhai等（2007）。自然449（7165）：1068-72。实验室在跑步成本和薪水中恢复了两次的运行费用和薪水，这使得五个经验合理。由新的不伦瑞克科学公司（New Brunswick Scientific），两个单元越来越难以获得剩余单元的备件，以购买3个新的发酵罐和一个细胞收获的离心设备，以取代旧单元，以便将其延续到未来的调查人员中，以提供有效的研究中的研究人员。 公共卫生相关性：调查人员利用UAB发酵设施的服务正在进行基本与健康相关的研究，以更好地了解各种人类疾病。这项研究的目的是彻底了解特定疾病的潜在机制，并将这些知识应用于开发更有效的治疗和患者护理中。