OLYMPUS FV1000 INVERTED SPECTRAL CONFOCAL SYSTEM

奥林巴斯 FV1000 倒光谱共焦系统

• 批准号: 7794254
• 负责人: JOHN A MERCER
• 金额: $ 37.48万
• 依托单位: MC LAUGHLIN RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-05 至 2011-08-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794254
• 关键词: Alzheimer' s Disease; Biomedical Research; Brain; Color; Conflict (Psychology); Confocal Microscopy; Demyelinating Diseases; Dyes; Embryo; Faculty; Funding; Hour; Image; Immunofluorescence Immunologic; In Situ Hybridization; Institution; Label; Manufacturer Name; Methods; Microscope; Natural regeneration; Nerve Fibers; Nervous System Trauma; Parkinson Disease; Peripheral Nerves; Peripheral Nervous System Diseases; Proteins; Request for Applications; Research; Research Institute; Schedule; System; programs

DESCRIPTION (provided by applicant): Confocal microscopy has become an important part of many biomedical research programs. The McLaughlin Research Institute last acquired a confocal unit (Bio-Rad MicroRadiance) twelve years ago. It has multiple limitations, including two colors, poor sensitivity, and lack of manufacturer support. Performing confocal microscopy at other research institutions requires more than a three-hour drive one way. This application requests funds for an Olympus FV1000 Spectral Confocal with IX81 inverted microscope and accessories. This microscope will allow imaging of peripheral nerve sections, teased nerve fibers, neurospheres, brain sections, and embryos labeled by in situ hybridization, immunofluorescence, vital dyes, and tagged proteins. These methods are critical to advancing NIH-funded projects studying mechanisms underlying nervous system injury and regeneration, Alzheimer's disease, Parkinson's disease, demyelinating diseases, and peripheral neuropathies. The addition of another microscope also will alleviate scheduling conflicts and allow for further expansion, with two new faculty planned in the next two years.

描述（由申请人提供）：共聚焦显微镜已成为许多生物医学研究项目的重要组成部分。麦克劳林研究所最后一次购买共聚焦装置 (Bio-Rad MicroRadiance) 是在十二年前。它有多种限制，包括两种颜色、灵敏度差以及缺乏制造商支持。在其他研究机构进行共焦显微镜检查单程需要三个多小时的车程。此申请需要资金购买配备 IX81 倒置显微镜和配件的奥林巴斯 FV1000 光谱共焦。该显微镜可对周围神经切片、神经纤维、神经球、脑切片和通过原位杂交、免疫荧光、活体染料和标记蛋白标记的胚胎进行成像。这些方法对于推进 NIH 资助的项目至关重要，这些项目研究神经系统损伤和再生、阿尔茨海默病、帕金森病、脱髓鞘疾病和周围神经病的机制。增加另一台显微镜也将缓解日程安排冲突并允许进一步扩展，计划在未来两年内增加两名新教师。