The Gastrointestinal Experimental Model Systems (GEMS) Core

胃肠实验模型系统 (GEMS) 核心

• 批准号: 10117232
• 负责人: NOAH Freeman SHROYER
• 金额: $ 17.6万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-03-01 至 2023-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10117232
• 关键词: 3-Dimensional; Advisory Committees; Animals; Barrett Esophagus; Biological Models; Biology; Cancer Patient; Cell Culture Techniques; Cells; Clinical; Collection; Colonic Adenoma; Colorectal Cancer; Consultations; Culture Media; Derivation procedure; Development; Differentiation and Growth; Digestive System Disorders; Disease; Education and Outreach; Experimental Models; Generations; Germ-Free; Gnotobiotic; Goals; Health; Hospitals; Human; Immigrant; Immunodeficient Mouse; Inflammatory Bowel Diseases; Institutional Review Boards; Instruction; Insurance; Intestines; Maintenance; Medical center; Medicine; Modeling; Mus; Organoids; Patients; Pediatrics; Physiology; Privatization; Production; Protocols documentation; Published Comment; Reagent; Recommendation; Research Personnel; Rodent; Sampling; Services; Specific qualifier value; Specimen; Surveys; Technology; Texas; Tissue Sample; Training; Uninsured; Veterans; Xenograft procedure; animal facility; animal tissue; base; biobank; college; cost; embryonic stem cell; gastrointestinal; human pluripotent stem cell; human tissue; improved; induced pluripotent stem cell; matrigel; member; monolayer; neoplastic; patient population; pre-clinical; success; two-dimensional

Project Summary The Gastrointestinal Experimental Model Systems (GEMS) core is a newly reorganized of the Texas Medical Center-Digestive & Disease Center (TMC-DDC) in 2015. Based on examination of prior usage, reviewer comments from the previous competitive renewal, and the recommendation of the external advisory committee, the GEMS core is organized to encompass 1) an enteroid/organoid subcore and 2) a gnotobiotic subcore. The DDC successfully obtained Baylor College of Medicine (BCM) support to establish a gnotobiotic animal facility during the previous Project Period. This reorganization was based on the exciting scientific developments in the field, our unique expertise, increased DDC investigator need and use of enteroids and gnotobiotic animals in conjunction with decreased use of the physiology services offered by the previous Integrative Biology Core. To accomplish these aims, we will provide services, including the following: • Human enteroids from the TMC-DDC biobank: as 3-dimensional enteroids, monolayers or transwells • Murine enteroids from the TMC-DDC biobank • Complete growth media or components for growth and differentiation of human and animal enteroids • Derivation of new primary enteroids from animals or human tissue specimens • Derivation of stably transduced enteroid lines • Human pluripotent stem cell-derived intestinal organoids, made from embryonic stem cells or iPS cells • Xenograft generation of enteroids/organoids into immunodeficient mice • Production and maintenance of germ-free rodents • Technical services for studies involving gnotobiotic rodents • Creation of germ-free mice de novo • Training, outreach, and consultative services Importantly, there is no other similar facility in the TMC or nearby in the region. This Core is highly synergetic with the other scientific cores of the TMC-DDC. For example, extensive cooperation with Core E (Clinical Core) facilitates the development and approval of new IRB protocols and collection/tracking of tissue samples required to establish new human enteroid lines. Evidence of the success of this interaction is apparent in the >120 enteroids lines that are already established and the continued accrual of new samples targeting specific patient populations.

项目摘要 胃肠道实验模型系统（GEMS）核心是德克萨斯州的新重组 2015年医疗中心消化与疾病中心（TMC-DDC）。 审阅者的评论以前的竞争续约以及外部的建议 咨询委员会，GEMS核心组织以涵盖1）肠道/器官子核心和2）a gnotobiotic子核。 DDC成功获得了贝勒医学院（BCM）支持以建立 上一个项目期间的gnotobiotic动物设施。这种重组是基于 该领域令人兴奋的科学发展，我们独特的专业知识，增加了DDC研究者的需求和使用 肠内和gnotobiotic动物以及提供的生理服务的使用减少 由先前的综合生物学核心。为了实现这些目标，我们将提供服务，包括 下列： •来自TMC-DDC生物库的人类肠to虫：作为三维的肠托动物，单层或transwells •来自TMC-DDC生物库的鼠肠to •完整的生长培养基或人类和动物肠道差异的成分 •从动物或人体组织标本中衍生新的原代肠杆菌 •稳定翻译的肠线的推导 •由胚胎干细胞或IPS制成的人类多能干细胞源性肠道器官 细胞 •异种移植物的肠托动物/器官在免疫缺陷的小鼠中 •无菌啮齿动物的生产和维护 •涉及Gnotobiotic啮齿动物的研究技术服务 •创建从头开始的无菌老鼠 •培训，外展和咨询服务 重要的是，TMC或该地区附近没有其他类似的设施。这个核心很高 与TMC-DDC的其他科学核心协同作用。例如，与核心的广泛合作 E（临床核心）促进了新的IRB协议的开发和批准，并收集/跟踪 建立新的人类肠道线所需的组织样品。这种互动成功的证据 在已经建立的> 120个肠线中很明显，并且持续的新产品 针对特定患者人群的样本。