Neuronal Physiology Core

神经生理学核心

基本信息

批准号：
10018882
负责人：
Paulette B. Goforth
金额：
$ 39.86万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-20 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10018882
关键词：
Advisory Committees Animals Biological Assay Body Weight Brain Calcium Calcium Signaling Cell Culture Techniques Cell model Cell physiology Cells Cellular Assay Collaborations Communication Complement Computer software Consultations Custom Data Data Analyses Data Collection Doctor of Philosophy Electrophysiology (science)Ensure Equipment Experimental Designs Feeding behaviors Fiber Food Intake Regulation Gastrectomy Gastrointestinal tract structure Genetic Genetic Models Human Resources Hypothalamic structure Image Imaging Techniques Individual Ions Label Laboratories Measurement Measures Metabolic Diseases Methodology Michigan Monitor Neurons Noise Output Photometry Physiological Physiological Processes Physiology Play Population Postdoctoral Fellow Preparation Process Protocols documentation Quality Control Regulation Reproducibility Research Design Research Project Grants Rodent Role Sample Size Secure Services Slice Standardization Stimulus Structure Students Supervision Techniques Technology Testing Training Universities Work bariatric surgery calcium indicator conditioned place preference electrical measurement experience experimental study extracellular fluorophore gut-brain axis hindbrain in vivo interest meetings mouse model neural circuit neuronal circuitry novel operation optogenetics patch clamp programs relating to nervous system response theories tool

项目摘要

Neuronal Physiology Core Abstract: The studies proposed in this Program Project will define the structure, function and regulation of hindbrain neural circuitry that underlies gut-brain communication and ascertain alterations that occur within this circuitry in response to bariatric surgery. To probe the function and regulation of distinct neuronal populations within this circuitry, at the cellular level, the Neuronal Physiology Core will conduct specialized assays of cellular function that are targeted to select neuronal subtypes using genetically encoded tools combined with unique genetic mouse models. These assays include 1. in vivo fiber photometry to measure calcium signals from genetically encoded calcium indicator (GECI)-expressing hindbrain neuronal populations, as a readout of cellular activity in response to stimuli, pre and post-VSG. 2. ex vivo electrophysiologial analysis of genetically-labeled and indentified neurons to directly assess cellular mechanisms underlying function and regulation pre and post vertical sleeve gastrectomy (VSG) 3.combined optogenetics and ex vivo electrophysiology to assess the functional connectivity of defined neuronal populations 4. in vivo optogenetic stimulation or inhibition of distinct genetically targeted neuronal populations to assess the impact of their modulation on physiologic processes (e.g. feeding behavior, conditioned place preference)

神经元生理核心抽象的：该计划项目中提出的研究将定义后脑神经电路是肠道沟通和确定改变的基础响应减肥手术而发生在该电路中。探测功能和调节在该电路中，在细胞水平上的不同神经元种群的神经元生理核心将进行细胞功能的专门测定，该测定旨在选择神经元使用遗传编码工具与独特的遗传小鼠模型相结合的亚型。这些测定包括1。体内纤维光度法，以测量遗传编码的钙信号钙指示剂（GECI）表达后脑神经元种群，作为细胞的读数响应刺激，前和VSG的活性。 2。离体电生理分析遗传标记和凹痕神经元直接评估基础的细胞机制功能和调节前后垂直套筒胃切除术（VSG）3. 光遗传学和离体电生理学评估定义的功能连通性神经元种群4。体内光遗传学刺激或抑制不同的遗传学有针对性的神经元种群评估其调节对生理的影响过程（例如，进食行为，条件地点偏好）