Neuronal Physiology Core

神经生理学核心

基本信息

批准号：
9792645
负责人：
Paulette B. Goforth
金额：
$ 37.22万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-20 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9792645
关键词：
Advisory Committees Animals Biological Assay Body Weight Brain Calcium Calcium Signaling Cell Culture Techniques Cell model Cell physiology Cells Cellular Assay Collaborations Communication Complement Computer software Consultations Custom Data Data Analyses Data Collection Doctor of Philosophy Electrophysiology (science)Ensure Equipment Experimental Designs Feeding behaviors Fiber Food Intake Regulation Gastrectomy Gastrointestinal tract structure Genetic Genetic Models Human Resources Hypothalamic structure Image Imaging Techniques Individual Ions Label Laboratories Measurement Measures Metabolic Diseases Methodology Michigan Monitor Neurons Noise Output Photometry Physiological Physiological Processes Physiology Play Population Postdoctoral Fellow Preparation Process Protocols documentation Quality Control Regulation Reproducibility Research Design Research Project Grants Rodent Role Sample Size Secure Services Slice Standardization Stimulus Structure Students Supervision Techniques Technology Testing Training Universities Work bariatric surgery calcium indicator electrical measurement experience experimental study extracellular fluorophore gut-brain axis hindbrain in vivo interest meetings mouse model neural circuit neuronal circuitry novel operation optogenetics patch clamp preference programs relating to nervous system response theories tool

项目摘要

Neuronal Physiology Core Abstract: The studies proposed in this Program Project will define the structure, function and regulation of hindbrain neural circuitry that underlies gut-brain communication and ascertain alterations that occur within this circuitry in response to bariatric surgery. To probe the function and regulation of distinct neuronal populations within this circuitry, at the cellular level, the Neuronal Physiology Core will conduct specialized assays of cellular function that are targeted to select neuronal subtypes using genetically encoded tools combined with unique genetic mouse models. These assays include 1. in vivo fiber photometry to measure calcium signals from genetically encoded calcium indicator (GECI)-expressing hindbrain neuronal populations, as a readout of cellular activity in response to stimuli, pre and post-VSG. 2. ex vivo electrophysiologial analysis of genetically-labeled and indentified neurons to directly assess cellular mechanisms underlying function and regulation pre and post vertical sleeve gastrectomy (VSG) 3.combined optogenetics and ex vivo electrophysiology to assess the functional connectivity of defined neuronal populations 4. in vivo optogenetic stimulation or inhibition of distinct genetically targeted neuronal populations to assess the impact of their modulation on physiologic processes (e.g. feeding behavior, conditioned place preference)

神经生理学核心抽象的：本计划项目中提出的研究将定义结构、功能和监管后脑神经回路是肠脑通讯的基础，并确定发生在该电路内以响应减肥手术。探究功能和调节在细胞水平上，该电路内不同的神经元群体，神经元生理学 Core 将进行专门的细胞功能分析，以选择神经元使用基因编码工具结合独特的基因小鼠模型来识别亚型。这些测定包括 1. 体内光纤光度测定法，测量基因编码的钙信号钙指示剂（GECI）表达后脑神经元群，作为细胞的读数 VSG 前后对刺激的反应活动。 2. 离体电生理分析基因标记和鉴定的神经元可直接评估潜在的细胞机制垂直袖状胃切除术 (VSG) 3.combined 前后的功能和调节光遗传学和离体电生理学来评估定义的功能连接 4. 体内光遗传学刺激或抑制不同遗传的神经元群体针对神经元群体来评估其调节对生理的影响过程（例如进食行为、条件性位置偏好）