Component: Husbandry and Management Core

组成部分：畜牧和管理核心

• 批准号: 10014301
• 负责人: Kamm Prongay
• 金额: $ 141.29万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10014301
• 关键词: AIDS-Related Opportunistic Infections; AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Animal Model; Animals; Artificial Insemination; Breeding; Caring; Cytomegalovirus; Derivation procedure; Development; Fostering; Future; HIV; Health; Herpesvirus 1; Housing; Human; Infant; Lymphocryptovirus; Macaca; Macaca mulatta; Maintenance; Measles; Monitor; Mothers; Partner in relationship; Pathogenesis; Population; Practice Management; Research; Resources; Rhadinovirus; Rhesus; SIV; Simian Foamy Virus; Simian Retroviruses; Testing; Time; Vaccines; Viral Vector; Virus; animal resource; base; experience; germ free condition; improved; innovation; novel; programs; social

PROJECT SUMMARY, Core A: Husbandry and Management Core Expanded SPF (eSPF) rhesus macaques (Macaca mulatta), which are defined as being negative to rhesus rhadinovirus, simian immunodeficiency virus, simian foamy virus, simian T-lymphotropic virus, simian retrovirus, macacine herpesvirus-1 (BV), measles, and rhesus cytomegalovirus, are necessary to study an increasing range of viruses important to human health, as well as the development and testing of novel viral vectors. eSPF macaques have become the premier animal model for HIV/AIDS research, including studies on HIV pathogenesis, AIDS-related opportunistic infections, and the development of HIV treatments and vaccines. Currently, the national need for genetically characterized eSPF rhesus macaques for HIV/AIDS research far exceeds the current availability. Therefore it is imperative that the available eSPF rhesus macaque populations grow in size. The successful maintenance of expanded SPF colonies is challenging, however, and requires strict management practices in order to remain specific pathogen free. With over 18 years’ experience in establishing and maintaining a colony of eSPF animals, the ONPRC is the leader at managing eSPF rhesus macaques. The overall aims of the Husbandry and Management Core are to breed and grow the ONPRC eSPF colony in order to meet existing and future animal resource demands, continue to provide and improve upon the existing exemplary eSPF husbandry and veterinary care, and to improve the eSPF resource by making the entire colony lymphocryptovirus negative. To do this we will provide innovative team based management practices including expansion of the colony into outdoor sheltered housing, introduction of a time- mated breeding program and artificial insemination, a foster mother program for abandoned infants, implementation of new enrichment and social monitoring strategies, and re-derivation of LCV negative infants from within the eSPF colony.

项目摘要，核心A：饲养和管理核心 扩展的SPF（ESPF）恒河猕猴（Macaca Mulatta），定义为恒河 rhadinovirus，Simian免疫缺陷病毒，Simian Foamy病毒，Simian T-淋巴机病毒，Simian 逆转录病毒，大甲酸疱疹病毒-1（BV），麻疹和恒河猴巨细胞病毒是必不可少的 增加对人类健康至关重要的病毒范围以及新型病毒的开发和检测 向量。 ESPF猕猴已成为艾滋病毒/艾滋病研究的主要动物模型，包括研究 HIV发病机理，与AIDS相关的机会感染以及HIV治疗和疫苗的发育。 目前，全国对遗传特征的ESPF恒河猕猴的艾滋病毒/艾滋病研究的需求遥不可及 超过当前的可用性。因此，必不可少的ESPF恒河猴必须 种群的大小增长。但是，成功维护扩大的SPF殖民地是具有挑战性的 并需要严格的管理实践才能保持特定的病原体。超过18年 在建立和维持ESPF动物殖民地的经验，ONPRC是管理的领导者 ESPF恒河猕猴。饲养和管理核心的总体目标是繁殖和发展 为了满足现有和未来的动物资源需求，继续提供和 改善现有的模范ESPF饲养和兽医护理，并改善ESPF资源 通过使整个菌落淋巴结病毒阴性阴性。为此，我们将提供创新的团队 管理实践，包括将殖民地扩展到室外庇护所住房，引入时间 - 伴侣育种计划和人工授精，这是一项针对废弃婴儿的寄养母亲计划， 实施新的丰富和社会监测策略，并重新启动LCV负面婴儿 来自ESPF殖民地。