ISOLATION & CHARACTERIZATION OF CARDIOACTIVE COMPOUNDS IN HI BOX JELLYFISH VENOM

隔离

基本信息

批准号：
7720348
负责人：
ANGEL ANNE YANAGIHARA
金额：
$ 3.75万
依托单位：
UNIVERSITY OF HAWAII AT MANOA
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-06-01 至 2009-05-31
项目状态：
已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Hawaiian box jellyfish (Carybdea alata) belong to the most notoriously toxic class of jellyfish, the cubozoans, which are commonly found in tropical and subtropical waters from Hawaii to Australia. Cubozoan stings are extremely painful and result in potentially life-threatening Irukandji syndrome, which presents as a constellation of systemic signs and symptoms, including cardiopulmonary distress. A retrospective clinical analysis of emergency room patients demonstrates that Irukandji syndrome occurs in about 5% of local box jellyfish sting victims. Still, the precise biochemical basis for this syndrome is unclear. In Hawaii, Australia and Indonesia, current therapy is limited to symptomatic care. This research proposal is prompted by our recent discovery of small molecules, chemically similarly to certain insect toxins, which induce multiple physiological effects including profound cardiac effects in both crayfish and mice. The specific aims of this project are to conduct focused efforts to purify, identify and physiologically characterize these highly cardioactive compounds. The overall goal is to provide much-needed insights into the mechanisms of action of these compounds and provide the basis for better clinical management.

该子项目是利用该技术的众多研究子项目之一资源由 NIH/NCRR 资助的中心拨款提供。子项目和研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金，因此可以在其他 CRISP 条目中表示。列出的机构是对于中心来说，它不一定是研究者的机构。夏威夷箱形水母 (Carybdea alata) 属于毒性最强的立方体水母，常见于从夏威夷到澳大利亚的热带和亚热带水域。立方体动物蜇伤极其疼痛，可能会导致危及生命的伊鲁坎吉综合征，该综合征表现为一系列全身症状和体征，包括心肺窘迫。对急诊室患者的回顾性临床分析表明，当地箱形水母蜇伤患者中约有 5% 患有伊鲁坎吉综合征。尽管如此，这种综合征的确切生化基础尚不清楚。在夏威夷、澳大利亚和印度尼西亚，目前的治疗仅限于对症治疗。这项研究提案是由我们最近发现的小分子推动的，这些小分子的化学性质与某些昆虫毒素相似，可引起多种生理效应，包括对小龙虾和小鼠的深远心脏影响。该项目的具体目标是集中精力纯化、鉴定这些高度心脏活性的化合物并对其进行生理学表征。总体目标是提供对这些化合物作用机制急需的见解，并为更好的临床管理提供基础。