Early Metazoan Nano-collagens for Promotion of Wound Healing

早期后生动物纳米胶原蛋白促进伤口愈合

基本信息

批准号：
8607898
负责人：
ANGEL ANNE YANAGIHARA
金额：
$ 29.67万
依托单位：
UNIVERSITY OF HAWAII AT MANOA
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-01-10 至 2015-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8607898
关键词：
Accidental Injury Acute Address Anemone Animal Model Binding Biochemistry Biocompatible Materials Biological Assay Biophysics Blood Circulation Blood Platelets Carybdea Cells Cellular Immunology Chymase Cicatrix Cnidaria Collagen Collagen Receptors Cubozoa Cutaneous Cysteine Data Decubitus ulcer Deposition Dermal Development Eicosanoids Employee Strikes Engineering Exhibits Eye Fibrin Fibroblasts Future Glutamine Glycine Grant Growth Factor Hawaiian population Healed Histology Human Immune response Immunologist In Vitro Incubated Inflammatory Inflammatory Response Integrins Jellyfish Lesion Life Mast Cell Stabilizer Mechanics Mediator of activation protein Medical Device Modality Modeling Molecular Molecular Analysis Mus NMR Spectroscopy Operative Surgical Procedures Organelles Peptide Hydrolases Peptides Phosphotransferases Positioning Attribute Post-Translational Protein Processing Preparation Proline Property Proteins Recovery Relative (related person)Research Resolution Sea Anemones Signal Transduction Speed Steel Sting Injury Structure Structure-Activity Relationship Tensile Strength Testing Therapeutic Time Tissues Toxin Translating Tryptase Ursidae Family Vascularization Vertebrates Wound Healing base biophysical properties capsule chemokine coral cytokine healing hemodynamics immunogenicity improved in vitro Assay in vivo innovation insight mast cell medical implant molecular dynamics molecular scale nano nanomaterials nanometer novel point of care pressure public health relevance receptor research study response scale up solid state nuclear magnetic resonance therapeutic development wound

项目摘要

DESCRIPTION (provided by applicant): The stinging-cell apparatus (nematocyst) of corals, anemone and jellyfish (Phylum Cnidaria) exhibits remarkable biophysical properties such that the discharge of the prey-piercing tubule occurs at bullet-like forces and speed. The nematocyst capsule wall and tubule are composed of highly specialized proteins, for which limited information is available. We have recently discovered that a novel, self-assembling, "nano- collagen", from the Hawaiian box jellyfish, Carybdea alata causes massive fibrin deposition by platelets and activates mast cell signaling, suggesting a remarkable potential to drive two key aspects of wound healing. The overall objective of the proposed research is to develop new wound-repair agents based on this novel peptide that is potently bioactive and exhibits attributes of a self-assembling nanomaterial. The central premise is that the robust fibrinogenic pro-inflammatory activity is necessary and sufficient to serve as a useful first- response wound-management and wound-healing agent. An interdisciplinary team of multiple PIs, comprising a toxin biochemist, an immunologist and a protein biophysicist, has been assembled to address key aspects of the therapeutic potential. Our proposal seeks to address two critical questions that precede therapeutic development of this unique biomaterial. First, we will characterize the biophysical and materials properties of this nano-collagen. Completion of this objective will position us to examine fabrication and delivery modalities. Second, we will evaluate and optimize the potential for beneficial and ameliorative promotion of local immune responses by this novel collagen. Completion of this objective will position us to understand the immunological consequences of the application of this material to wounds. At the conclusion of the grant period, we will be positioned to translate our paradigm-shifting approach of using an ancient early metazoan self-assembling collagen to heal wounds in humans. The proposed research is innovative because of the high potential of developing potent nanomaterials, based on a natural nano-collagen, to promote wound management and accelerate wound healing in humans. The availability of such improved applications would have an immediate impact on point-of-care response to severe life-threatening dermal wounds, resulting from acute accidental injuries or cutaneous lesions, such as decubitus ulcers, from compromised circulation.

描述（由申请人提供）：珊瑚、海葵和水母（刺胞动物门）的刺细胞装置（线虫囊）表现出显着的生物物理特性，使得刺穿猎物的小管以子弹般的力量和速度进行放电。线虫囊壁和小管由高度专业化的蛋白质组成，但可获得的信息有限。我们最近发现，来自夏威夷箱形水母 Carybdea alata 的一种新型自组装“纳米胶原蛋白”会引起血小板大量纤维蛋白沉积并激活肥大细胞信号传导，这表明它具有推动伤口愈合的两个关键方面的巨大潜力。拟议研究的总体目标是开发基于这种新型肽的新型伤口修复剂，该肽具有强大的生物活性并表现出自组装纳米材料的属性。中心前提是，强大的纤维蛋白促炎活性对于作为有用的第一反应伤口管理和伤口愈合剂是必要且充分的。一个由多个 PI 组成的跨学科团队（包括毒素生物化学家、免疫学家和蛋白质生物物理学家）已组建起来，以解决治疗潜力的关键方面。我们的提案旨在解决这种独特生物材料的治疗开发之前的两个关键问题。首先，我们将表征这种纳米胶原蛋白的生物物理和材料特性。完成这一目标将使我们能够检查制造和交付方式。其次，我们将评估和优化这种新型胶原蛋白有益和改善局部免疫反应的潜力。完成这一目标将使我们能够了解将该材料应用于伤口的免疫学后果。在资助期结束时，我们将能够转化我们的范式转变方法，即使用古老的早期后生动物自组装胶原蛋白来治愈人类伤口。拟议的研究具有创新性，因为开发基于天然纳米胶原的有效纳米材料在促进人类伤口管理和加速伤口愈合方面具有巨大潜力。这种改进的应用的可用性将对严重危及生命的皮肤伤口的护理点反应产生直接影响，这些皮肤伤口是由急性意外伤害或循环受损引起的皮肤损伤（例如褥疮溃疡）引起的。

项目成果

期刊论文数量（1）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Phylogenetic analysis of higher-level relationships within Hydroidolina (Cnidaria: Hydrozoa) using mitochondrial genome data and insight into their mitochondrial transcription.

使用线粒体基因组数据并深入了解其线粒体转录，对 Hydroidolina（刺胞动物：Hydrozoa）内的高级关系进行系统发育分析。

DOI：
发表时间：
2015
期刊：
影响因子：
2.7
作者：
Kayal, Ehsan;Bentlage, Bastian;Cartwright, Paulyn;Yanagihara, Angel A;Lindsay, Dhugal J;Hopcroft, Russell R;Collins, Allen G
通讯作者：
Collins, Allen G