Biorepository and Animal Core

生物样本库和动物核心

• 批准号: 10026254
• 负责人: Matthew C Cave
• 金额: $ 17.1万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-10 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10026254
• 关键词: Address; Alcoholic Liver Diseases; Animal Experimentation; Animal Model; Animals; Autopsy; Biological; Biology; Centers of Research Excellence; Characteristics; Data; Development; Ensure; Equipment and supply inventories; Evaluation; Experimental Animal Model; Experimental Models; Fees; Fostering; Future; Germ-Free; Gnotobiotic; Goals; Hepatocyte; Histology; Human; Human Resources; IACUC; In Vitro; Individual; Infrastructure; Institution; Institutional Review Boards; Liver; Liver diseases; Maintenance; Malignant neoplasm of liver; Measures; Mission; Modeling; Mus; Occupational; Operative Surgical Procedures; Phenotype; Procedures; Protocols documentation; Research; Research Personnel; Resources; Sampling; Service delivery model; Services; Specimen; Standardization; Structure; System; Techniques; Tissue Banks; Toxicology; Training; Training and Education; Translational Research; biobank; biological specimen archives; career development; design; experimental study; flexibility; gut microbiome; human subject; interest; investigator training; meetings; member; metabolic phenotype; sample collection; success

Project Summary Abstract – Animal Model and Biorepository Core (Core D) The Animal Model and Biorepository Core (Core D) of the Hepatobiology and Toxicology Center provides the essential expertise, facilities, resources, and standardized services required for impactful liver research. The Core focuses on the needs of the COBRE’s junior investigators, but it also serves other Center members and liver investigators. The Core enhances infrastructure as well as the technical and academic capabilities of the Center and the Institution as a whole. Core D’s primary functions are to provide COBRE members with access to: (i) animal models of liver disease (including the characterization of these models); (ii) primary liver cells; and (iii) previously collected, de-identified biological specimens from human subjects with liver disease. The Core’s experimental model services include: animal exposure; husbandry; necropsy with tissue collection, processing and biorepository storage; phenotyping and liver histology; as well as the isolation of primary hepatic cells for ex vivo and in vitro studies. The Core will assist COBRE members in developing new experimental animal models and in vitro systems to study liver disease. The Core will serve as a liaison to non-duplicative animal research resources at UofL’s two other COBRE Centers to maximize overall impact. Core personnel will ensure uniformity of husbandry as well as sample collection, processing and evaluation so that the same set of characteristics is measured in each model. This uniformity ensures that the data generated within the COBRE are consistent and that results of one experiment will be directly comparable with the results of another. The Core’s human biorepository enables access to a unique inventory of previously collected de-identified biological samples from subjects with liver diseases (e.g., alcoholic liver disease, occupational/environmental liver diseases, liver cancer, etc.) in order to promote translational research. Core D will assist COBRE members with the necessary regulatory approvals for their research. In addition to its function as a service core, Core D will promote the education and training of the COBRE junior investigators and members. To function as a vital integrative component of the COBRE providing services not only for its members but also for the Institution as a whole, Core D has the following Specific Aims: 1) To foster the development and success of current and future COBRE projects. 2) To educate and train investigators. 3) To develop new protocols and techniques. 4) To become an integral and self-sustaining component of the COBRE.

项目摘要摘要 - 动物模型和生物座核（核心D） 肝病学和毒理学中心的动物模型和生物座核（核心D）提供了 有影响力的肝脏研究所需的基本专业知识，设施，资源和标准化服务。 核心重点关注毛cre的初级调查人员的需求，但也为其他中心成员提供服务 肝脏研究人员。核心增强了基础设施以及技术和学术能力 中心和整个机构。核心D的主要功能是为毛病成员提供访问权限 至：（i）肝病的动物模型（包括这些模型的表征）； （ii）原发性肝细胞；和 （iii）先前从人类患有肝病的受试者中收集的，被取消识别的生物标本。 核心的实验模型服务包括：动物暴露；畜牧尸检与组织收集， 处理和生物座席存储；表型和肝组织学；以及主要肝的隔离 用于离体和体外研究的细胞。核心将帮助毛毛商成员开发新的实验 动物模型和研究肝病的体外系统。核心将成为非副本的联络 UOFL的动物研究资源在另外两个山羊中心，以最大程度地影响整体影响。核心人员会 确保饲养的统一性以及样本收集，处理和评估，以便相同 在每个模型中测量特征。这种均匀性确保了毛绒内生成的数据 是一致的，一个实验的结果将与另一个实验的结果直接相当。 Core的人类生物座席可以访问先前收集的De-SIDIED BIOLOGIC的独特库存 肝病患者的样本（例如酒精肝病，职业/环境肝脏 疾病，肝癌等，以促进翻译研究。核心D将协助毛绒成员 他们的研究必要的监管批准。除了其作为服务核心的功能外，Core D还将 促进柯布尔初级调查员和成员的教育和培训。 充当毛绒的重要综合组成部分，不仅为其成员提供服务，而且还提供服务 对于整个机构，核心D具有以下具体目的：1）促进发展和成功 当前和未来的山毛项目。 2）教育和培训调查人员。 3）制定新协议和 技术。 4）成为毛病的组成部分和自我维持的组成部分。