Life Course Cancer Epidemiology Cohort in Women

女性生命历程癌症流行病学队列

基本信息

批准号：
10001441
负责人：
A. Heather Eliassen
金额：
$ 240.32万
依托单位：
HARVARD SCHOOL OF PUBLIC HEALTH
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-06-01 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10001441
关键词：
Age Archives Benign Big Data Biological Markers Biopsy Blood Breast Cancer survivor Cells Cessation of life Cheek structure Cloud Computing Cohort Analysis Collaborations Collection Complex Computers DNA Data Data Analytics Data Collection Data Management Resources Database Management Systems Diagnosis Diagnostic Diet Disease Progression Enrollment Erythrocytes Exposure to Factor Analysis Funding Gene Expression Gene Expression Profiling Genetic Genetic Predisposition to Disease Genetic Transcription Immunohistochemistry Incidence Individual Infant Infrastructure Laboratories Language Lesion Leukocytes Life Cycle Stages Life Style Malignant Neoplasms Malignant neoplasm of ovary Mammography Measures Mediating Medical Menopause Methods Mothers Normal tissue morphology Nurses&apos Health Study Paper Participant Pathology Perinatal Exposure Persons Pharmaceutical Preparations Phase Physical activity Plasma Postmenopause Premenopause Publications Publishing Quality of life Reproducibility Research Personnel Resource Sharing Resources Risk Factors Sampling Secure Smoking Specimen Tissue Microarray Tissues Tumor Tissue Update Urine Weight Woman analytical tool biobank breast cancer diagnosis cancer epidemiology cancer risk cancer survival case control cohort data management data sharing diagnostic screening fecal microbiome follow-up genetic epidemiology genome wide association study gut microbiome high school improved in utero indexing investigator training lifestyle factors malignant breast neoplasm melanoma member metabolomics microbiome analysis mortality tissue resource tumor usability

项目摘要

We propose to continue the follow-up of the Nurses' Health Study II (NHSII), a Cancer Epidemiology Cohort of 116,430 women enrolled at ages 25 to 42 years in 1989. The NHSII is a unique cohort in many ways, as the only large cohort of women enrolled before menopause and with nearly 30 years of repeated measures of exposures. We have information on smoking, weight, medication use, diet, physical activity and medical diagnoses updated every two to four years. Further, we have collected detailed data on in utero and infant exposures (from participants' mothers), diet during high school, and other unique exposure information. We have nearly complete ascertainment of deaths using the National Death Index and other methods. The follow- up of the NHSII cohort remains high, with 94% cumulative follow-up. The NHSII biorepository includes plasma, red blood cells, white blood cells, and first morning urine samples from 29,611 women; two thirds of these women contributed timed follicular and luteal premenopausal samples. In 2008 to 2011, 16,510 women provided second blood and urine samples when the majority were postmenopausal. We also have cheek cell DNA from an additional 29,859 women. Adding to these resources, we were recently funded to collect fecal samples from 25,000 participants for microbiome analysis. We have collected archival tissue blocks for incident breast and ovarian cancers and melanoma (approximately 70% of requests have been obtained), and collections for other cancers are ongoing. Breast and ovarian cancer tissues have been utilized for gene expression, and tissue microarrays are used extensively for immunohistochemistry. Prediagnostic mammograms have been collected for breast cancer cases and controls, as well as pathology material from previous biopsies of benign lesions. Substantial proportions of the cohort have GWAS and metabolomic data from nested case-control analyses. This cohort has been highly productive; over 330 papers have been published during the last five years, and the rate of publication is increasingly rapidly. With our active resource sharing component, we have participated in 19 consortia as a member of the NCI Cohort Consortium, one of the few cohorts contributing substantial data on premenopausal women and the largest contributor of premenopausal breast cancers. We also have active data sharing collaborations with individual external investigators. These external collaborations and consortia have resulted in more than 63 publications during the current funding period. In the current proposal, we aim to continue cohort follow-up and maintain these unique resources. We will continue collecting blood and urine samples from women with breast cancer who also provided prediagnostic samples, tumor tissue for incident cancers, and pre-diagnostic pre- and postmenopausal mammograms. We propose to conduct a long-term (2-3 year) reproducibility study for gut microbiome. Finally, we will enhance our data management and analytic tools to maximize efficiency and use of our complex resources to identify modifiable determinants of cancer incidence and survival.

我们建议继续跟踪护士健康研究 II (NHSII)，这是一个癌症流行病学队列 1989 年，有 116,430 名年龄在 25 至 42 岁之间的女性加入。NHSII 在很多方面都是一个独特的群体，因为只有大量女性在绝经前入组，并且经过近 30 年的重复测量曝光。我们有关于吸烟、体重、药物使用、饮食、身体活动和医疗的信息诊断每两到四年更新一次。此外，我们还收集了有关子宫内和婴儿的详细数据暴露（来自参与者的母亲）、高中期间的饮食以及其他独特的暴露信息。我们使用国家死亡指数和其他方法几乎完全确定了死亡情况。接下来的—— NHSII 队列的随访率仍然很高，累计随访率为 94%。 NHSII 生物储存库包括血浆、来自 29,611 名女性的红细胞、白细胞和第一次晨尿样本；其中三分之二妇女提供了定时卵泡和黄体绝经前样本。 2008 年至 2011 年，16,510 名女性当大多数人处于绝经后时，提供了第二份血液和尿液样本。我们也有颊细胞来自另外 29,859 名女性的 DNA。除了这些资源之外，我们最近还获得资助来收集粪便来自 25,000 名参与者的样本用于微生物组分析。我们收集了档案组织块乳腺癌、卵巢癌和黑色素瘤事件（约 70% 的请求已获得），以及其他癌症的收集工作正在进行中。乳腺癌和卵巢癌组织已被用于基因检测表达和组织微阵列广泛用于免疫组织化学。预诊断已收集了乳腺癌病例和对照的乳房 X 光检查，以及来自以下组织的病理材料：既往良性病变的活检。相当大比例的队列拥有 GWAS 和代谢组学数据来自嵌套病例对照分析。这群人的生产力非常高；已发表论文330余篇近五年来出版，而且出版速度越来越快。凭借我们的活跃资源共享部分，我们作为 NCI Cohort Consortium 的成员参与了 19 个联盟，该联盟是提供有关绝经前妇女大量数据的少数群体和最大的贡献者绝经前乳腺癌。我们还与外部个人进行积极的数据共享合作调查人员。这些外部合作和联盟在本次会议期间发表了超过 63 篇出版物当前的资助期限。在当前的提案中，我们的目标是继续队列随访并维持这些独特的资源。我们将继续收集患有乳腺癌的女性的血液和尿液样本还提供了预诊断样本、突发癌症的肿瘤组织以及预诊断前和预绝经后乳房X光检查。我们建议对肠道进行长期（2-3年）的可重复性研究微生物组。最后，我们将增强我们的数据管理和分析工具，以最大限度地提高效率和使用率利用我们复杂的资源来确定癌症发病率和生存率的可改变的决定因素。