Biospecimen-Core

生物样本核心

• 批准号: 10682550
• 负责人: SAYEED IKRAMUDDIN
• 金额: $ 20.41万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10682550
• 关键词: Adipocytes; Adipose tissue; Age; Attenuated; Automobile Driving; Benign; Bile fluid; Biological; Biology; Blood; Body Weight decreased; CDKN2A gene; Cells; Chemistry; Chronic Disease; Clinic; Collection; Confidentiality of Patient Information; Consent; County; Data; Data Analyses; Department chair; Development; Disease; Ensure; Epidemiology; Ethics; Ethnic Origin; Fatty Liver; Freezing; Fresh Tissue; Funding; Gender; Goals; Grant; Health; Health system; Hepatocyte; Hospitals; Human; Individual; Infertility; Inflammation; Insulin Resistance; Intervention Studies; Laboratories; Lesion; Liver; Longevity; Mammals; Maps; Measures; Medical Records; Medicine; Meta-Analysis; Metabolic; Metabolic syndrome; Metabolism; Minnesota; Morbidity - disease rate; Mus; Muscle; Operative Surgical Procedures; Organ; Organ Donor; Ovarian Tissue; Ovary; Participant; Pathology; Patient Care; Patient Recruitments; Patients; Peripheral Blood Mononuclear Cell; Pharmaceutical Preparations; Process; Protocols documentation; Quality Control; Race; Research; Research Personnel; Role; Sampling; Secure; Serum; Site; Skeletal Muscle; Socioeconomic Status; Specimen; Stress; Therapeutic; Tissue Banks; Tissue Sample; Tissues; Transplantation; Tumor Suppressor Proteins; age related; aging population; bariatric surgery; biobank; biological specimen archives; cell type; ethical, legal, and social implication; ethnic diversity; fatty liver disease; frailty; healthy aging; human tissue; improved; intravenous glucose tolerance test; legal implication; model organism; mortality; mouse model; participant enrollment; peripheral blood; pharmacologic; professor; prospective; quality assurance; recruit; research study; response; sample collection; senescence; skeletal; social implication; socioeconomics; spatiotemporal; stem cells; tissue mapping; wound healing

Project Summary Senescent cells (SnCs) accumulate with age and in various chronic disease states and clearly contribute to driving morbidity and mortality in model organisms. Drugs that selectively eliminate SnCs (“senolytics”) were first developed by our team and have been demonstrated to reduce frailty, extend median lifespan, and attenuate morbidity in murine models of numerous age-related diseases. However, SnCs also play a role in wound healing and tissue remodeling. To advance senolytics to their greatest potential, then, it is imperative to understand more about SnCs in humans with healthy aging. As part of SenNet, the proposed Minnesota Tissue Mapping Center (MN TMC) has selected four tissues for identification, characterization, and spatio-temporal analysis of senescence: adipose, skeletal muscle, liver, and ovarian tissues. These tissues were selected because of the scientific expertise at UMN and Mayo Clinic in the biology of these organs, evidence of increased senescence with age, and availability of biobanked specimens as well as surgical access to fresh tissues from healthy individuals. The overall goal of the Biospecimen Core (BSP), co-directed by Dr. Sayeed Ikramuddin, Chair of the UMN Department of Surgery, and Dr. Oyedele Adeyi, Professor of Laboratory Medicine and Pathology, is to provide the tissues and blood for PBMC isolation to the Biological Analysis Core (BAC) for bulk, single cell, and spatial analysis of human SnCs for subsequent multi-plex analysis of the results by the Data Analysis Core (DAC). The BSP will leverage their strong track record of obtaining high-quality, well-annotated human samples from non-chronically diseased individuals who are metabolically characterized and with appropriate diversity in gender, race, socio-economic status, ethnicity, and other relevant diversity parameters. The BSP will also provide access to UMN’s specimen procurement network (BioNet) that has ~70,000 archival tissues (frozen, FFPE) and biofluids (frozen) collected for research, including several adipose depots, several muscles, liver from organ donors, and healthy ovarian tissues removed because of benign lesions. In addition, the BSP has access to tissues within the Rochester Epidemiology Project, which encompasses the medical records of all citizens living in Olmsted County, MN since 1966, along with archived biological specimens. The Aims of the BSP are to: 1) Prepare and maintain all regulatory materials supporting human tissue collection; 2) Recruit and consent patients of different ages for this project; 3) Characterize the metabolic health of enrolled participants; 4) Coordinate with BioNet for intraoperative specimen collection, annotation, and storage of tissues; 5) Procure and annotate high quality target tissues along with relevant biofluids; 6) Longitudinally follow patients for which weight loss and bariatric surgery are metabolic perturbations; 7) Disseminate the samples to MN TMC BAC; and 8) Partner with SenNet to share tissues and to develop shared patient consent and tissue collection protocols with appropriate Ethical, Legal and Social Implications and quality control and assurance considerations.

项目摘要 衰老细胞（SNC）随着年龄和各种慢性疾病状态而积聚，明显有助于 驱动模型生物的发病率和死亡率。有选择地消除SNC（“塞溶剂”）的药物是首先 由我们的团队开发，并已被证明是为了减少脆弱，延长中位寿命并衰减 许多与年龄相关疾病的鼠模型中的发病率。但是，SNC在伤口愈合中也起作用 和组织重塑。为了使Senolottics发挥最大的潜力，必须了解更多 关于健康衰老的人类的SNC。作为Sennet的一部分，拟议的明尼苏达州组织映射中心 （MN TMC）选择了四个组织，用于识别，表征和时空分析 衰老：脂肪，骨骼肌，肝脏和卵巢组织。选择这些组织是因为 在这些器官的生物学中，UMN和Mayo诊所的科学专业知识，具有增加感应的证据 随着年龄的增长，生物群的可用性以及可从健康的外科手术访问新鲜时间 个人。 BioScecimen Core（BSP）的总体目标，由Sayeed Ikramuddin博士主席共同执导。 UMN外科系和实验室医学与病理学教授Oyedele Adeyi博士是 为了向散装，单细胞的生物分析核心（BAC）分离PBMC的组织和血液 和人类SNC的空间分析，以随后通过数据分析核心对结果进行多重量分析 （DAC）。 BSP将利用他们的良好轨道 获得高质量，良好的人类样品的记录 来自代谢表征并具有适当多样性的非偶然脱离的个体 性别，种族，社会经济地位，种族和其他相关多样性参数。 BSP也将 提供访问权限 UMN的标本采购网络（Bionet）具有约70,000个档案组织（冷冻， FFPE）和生物流体（冷冻）进行研究，包括几种脂肪沉积物，几种肌肉，肝脏，来自 由于良性病变，器官捐献者和健康的卵巢组织被去除。此外，BSP可以访问 到罗切斯特流行病学项目中的组织，该项目涵盖了所有公民的病历 自1966年以来，居住在明尼苏达州奥尔姆斯特县，以及存档的生物标本。 BSP的目的是 至：1）准备和维护所有支持人体组织收集的监管材料； 2）招募和同意 该项目不同年龄的患者； 3）特征是入学参与者的代谢健康； 4） 与Bionet进行术中标本的收集，注释和储存组织； 5）采购和 注释高质量的目标时间以及相关的生物流体； 6）纵向跟随患者的体重 损失和减肥手术是代谢扰动； 7）将样品传播到Mn TMC BAC中；和8） 与Sennet合作以共享组织并与 适当的道德，法律和社会影响以及质量控制和保证考虑。