PROTEOMICS CORE

蛋白质组学核心

基本信息

批准号：
7699757
负责人：
MICHAEL ELDON GREENBERG
金额：
$ 19.2万
依托单位：
BOSTON CHILDREN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-07-01 至 2011-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7699757
关键词：
Accounting Acetylation Affinity Chromatography Algorithms Amines Amino Acid Sequence Area Arts Attention Avidin Back Biopolymers Biotin Blood capillaries Buffers Cell Fraction Cell physiology Cells Charge Child Code Complex Computer Simulation Computer software Computers Condition Consultations Core Facility Count Coupled Cyclotrons Cysteine DNA Sequence Daily Data Data Analyses Data Files Databases Detection Deuterium Development Developmental Disabilities Digestion Dimensions Disease Electrophoresis Electrospray Ionization Ensure Equipment Event Exclusion Explosion Fee-for-Service Plans Flavoring Floor Fourier Transform Frequencies Future Gel Goals Healthcare Heating High Pressure Liquid Chromatography Housing Human Resources Hybrids Hydrogen Image Iodoacetamide Ion-Exchange Chromatography Procedure Ions Isotopes Label Lasers Lead Liquid Chromatography Manufacturer Name Mass Spectrum Analysis Measures Mental Retardation and Developmental Disabilities Research Centers Methods Methylation Modification Molecular Molecular Weight Molecular and Cellular Biology Nervous system structure Neurobiology Neurologic Neurons Neurosciences Neurosciences Research Numbers Ocular orbit Organism Pediatric Hospitals Peptide Sequence Determination Peptides Phase Phosphorylation Plasmids Population Post-Translational Protein Processing Postdoctoral Fellow Preparation Procedures Process Protein Isoforms Proteins Proteomics Protocols documentation Quality Control Range Reagent Recombinant Proteins Rehydrations Relative (related person)Research Research Personnel Resolution Sampling Scanning Science Scientist Services Side Signal Transduction Silver Staining Solutions Source Spectrometry Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spottings Stable Isotope Labeling Staging Staining method Stains Students Sulfhydryl Compounds System Techniques Technology Temperature Time Tissues Training Two-Dimensional Gel Electrophoresis analytical tool base capillary cost dalton data acquisition deamidation density design desire glycosylation improved instrument instrumentation interest ionization magnetic field mass spectrometer member molecular mass nano-electrospray novel numb protein professor programs protein expression research study tandem mass spectrometry theories tool ultra high resolution voltage

项目摘要

2.a.2. Overall Objective The Proteomics Core aims to provide MRDDRC investigators with access to state of the art technologies in mass spectrometry and microscale capillary high performance liquid chromatography (proteomics) required for advanced studies in the cell and molecular biology of neurological developmental disabilities. Dr Greenberg (the Director of this MRDDRC) will be the Director of the Core. Dr. Jebanathirajah, a new Assistant Professor in the Neurobiology Program at Children's Hospital, and an expert in the field of mass spectrometry, will be the Manager responsible for supervising all Core activities. Major goals of the Core will be to use the techniques of mass spectrometry to assist MRDDRC investigators in the characterization of protein complexes that are relevant to neuronal function, to characterize changes in protein composition that occur in the nervous system during development and under conditions that lead to developmental disabilities, and to identify and characterize the function of post-translational modification of neuronal proteins. The Proteomics Core is actively involved in research in the area of mass spectrometry and any newly developed tools and methods will be made available to the MRDDRC investigators. The Core will also provide and develop proteomics related statistical and data handling tools.

2.a.2.总体目标蛋白质组学核心旨在为 MRDDRC 研究人员提供最先进的技术所需的质谱分析和微量毛细管高效液相色谱（蛋白质组学）神经发育障碍的细胞和分子生物学的高级研究。格林伯格博士（本 MRDDRC 主任）将担任核心主任。 Jebanathirajah 博士，新任助理教授儿童医院神经生物学项目的博士，以及质谱领域的专家，将是经理负责监督所有核心活动。核心的主要目标是使用这些技术质谱法协助 MRDDRC 研究人员表征蛋白质复合物与神经元功能相关，表征神经系统中蛋白质组成的变化在发育期间和导致发育障碍的条件下，并识别和表征神经元蛋白质翻译后修饰的功能。蛋白质组学的核心是积极参与质谱领域的研究以及任何新开发的工具和方法提供给 MRDDRC 调查人员。该核心还将提供和开发蛋白质组学相关的统计和数据处理工具。