CORE--Genomics-Proteomics

核心--基因组学-蛋白质组学

基本信息

批准号：
7491170
负责人：
Christophe Depre
金额：
$ 25.52万
依托单位：
UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-01 至 2011-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7491170
关键词：
2&apos -deoxycytidine 5&apos -triphosphate ADP ribosylation Accounting Acetonitriles Acetylation Acids Acrylamide Acrylamides Actins Address Affinity Affinity Chromatography Agar Gel Electrophoresis Age Albumins Aldehydes Aliquot Am 80 Amino Acid Sequence Amino Acids Ampholytes Antibodies Antibody Formation Antisense RNA Arts Bacteria Binding Binding Proteins Bioinformatics Biological Biological Assay Biometry Biotechnology Biotin Blast Cell Blood capillaries Buffers Calcium Caliber Calibration Capillary Electrophoresis Cardiac Cardiac Myocytes Cardiovascular system Caseins Cell Fraction Cell Fractionation Cell Nucleus Cell membrane Cell physiology Cells Centrifugation Characteristics Charge Chimeric Proteins Chloroform Chromatography Class Classification Cleaved cell Cloning Cluster Analysis Collaborations Compatible Complementary DNA Complex Computer software Condition Coomassie blue Core Facility Coupled Custom Cyclophilins Cytolysis Cytosol D Cells DNA DNA Microarray Chip DNA Polymerase I DNA Sequence Data Data Analyses Data Collection Databases Densitometry Depth Detection Devices Digestion Dimensions Disadvantaged Disease Doctor of Philosophy Double-Stranded RNA Dyes Edetic Acid Electrophoresis Endopeptidases Enzyme-Linked Immunosorbent Assay Epitopes Equipment Evaluation Excision Expressed Sequence Tags Family suidae Fluorescein Fluoresceins Fluorescence Fluorescent Dyes Fluorescent Probes Formaldehyde Fractionation Freezing Funding Gallium Gases Gel Gel Chromatography Gene Expression Gene Expression Regulation Gene Proteins Generic Drugs Genes Genetic Genome Genomics Genus Lynx Glutathione S-Transferase Glycerol Harvest Heart Heating High Pressure Liquid Chromatography Hour House mice Housekeeping Housekeeping Gene Housing Hydration status Hydrophobic Interactions Image Image Analysis In Situ In Situ Hybridization In Vitro Incubated Incubators Individual Iodoacetamide Ion Exchange Ions Isoelectric Focusing Isoelectric Point Isotopically-Coded Affinity Tagging Knock-out Label Laboratories Laemmli buffer Lasers Length Libraries Life Ligation Link Liquid Chromatography Liquid substance MALDI-TOF Mass Spectrometry Maintenance Maps Mass Chromatography Mass Spectrum Analysis Measurement Measures Mediating Membrane Messenger RNA Metals Methods Microarray Analysis Mitochondria Modeling Modification Molecular Molecular Cloning Molecular Probes Molecular Weight Monitor Monkeys Mus Nitrogen Nucleic Acids Numbers Nuts Nylons Oligonucleotides Online Systems Oranges Paraffin Parents Pathology Pattern Peptide Fragments Peptide Hydrolases Peptide antibodies Peptides Peptidylprolyl Isomerase Phase Phenols Phosphopeptides Phosphoproteins Phosphoric Monoester Hydrolases Phosphorylated Peptide Phosphorylation Phosphorylation Site Phosphotyrosine Physiology Play Poly(A)+ RNA Polyacrylamide Gel Electrophoresis Polymerase Polymerase Chain Reaction Polymers Post-Translational Protein Processing Preparation Principal Investigator Printing Probability Procedures Process Promega Protein Analysis Protein Array Analysis Protein Databases Protein Overexpression Proteins Proteome Proteomics Protocols documentation Publishing Purpose Pus Pyroxylin RNA RNA Processing RNA purification RNA-Directed DNA Polymerase Range Reaction Reading Reagent Regulation Relative (related person)Reporting Reproducibility Research Research Personnel Resolution Reverse Transcriptase Polymerase Chain Reaction Ribosomal Proteins Robot Robotics Role Running Sampling Scanning Screening procedure Sepharose Sequence Analysis Serine Services Signal Transduction Signal Transduction Pathway Silver Silver Staining Site Sodium Dodecyl Sulfate-PAGE Solutions Sorting - Cell Movement Source Specificity Spectrometry Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Speed Sports Spottings Staining method Stains Standards of Weights and Measures Structure Sucrose Sus scrofa SwissProt System Techniques Technology Testing Thiocyanates Thiourea Thrombin Time Tissue Sample Tissues Transcript Transgenic Mice Transgenic Organisms Translations Transmembrane Domain Trifluoroacetic Acid Triton X100 Trypsin Two-Dimensional Gel Electrophoresis United States National Institutes of Health Urea Validation Variant Vendor Water Western Blotting Work Yeasts amino group ammonium bicarbonate austin base cDNA Library capillary cellular targeting cinnamic acid comparative cost cross reactivity cyanine dye 5 data acquisition day design detector dimer fluorophore formamide gel electrophoresis gene cloning genome sequencing glycosylation human TYRP1 protein immunocytochemistry improved in vivo inhibitor/antagonist insight instrument interest mass spectrometer microbial alkaline proteinase inhibitor molecular dynamics mouse genome mouse model nano novel nucleic acid quantitation oxidation pH gradient phosphatase inhibitor polyclonal antibody polyvinylidene fluoride programs promoter protein aminoacid sequence protein expression protein function protein protein interaction research study reversed phase chromatography sample collection small molecule sodium citrate statistics synthetic peptide tandem mass spectrometry thiocyanate two-dimensional vector yeast two hybrid system

项目摘要

The Genomics/Proteomics Core is designed to support all the Projects in the Program Project for the Specific Aims that address the regulation of gene and protein expression. The Genomics/ Proteomics Core is also designed to interact with all the other Cores, especially Core-D (Biostatistics/Bioinformatics). Genomics and Proteomics are combined to offer a link, both logical and biological, between gene expression, transcript stability, protein translation and post-translational modifications. The Core will combine focused techniques (to investigate one specific product of interest) and large-scale profiling at both the gene and protein levels. The Genomics/ Proteomics Core will provide technical assistance to the different Projects for the processing of samples, whereas the preparation of the samples themselves will be the responsibility of the investigators from the different Projects and from Core B (Physiology). Once the samples are processed, the raw data will be transmitted to these investigators and to Core D (Biostatistics) who will be in charge of the data analysis and extraction of results. Additional techniques related to gene and protein expression that would require fixed samples will be done in collaboration with Core E (Pathology).

基因组学/蛋白质组学核心旨在支持该计划项目中的所有项目解决基因和蛋白质表达调控的具体目标。基因组学/蛋白质组学核心还设计用于与所有其他核心交互，特别是 Core-D（生物统计学/生物信息学）。基因组学和蛋白质组学相结合，提供了基因之间的逻辑和生物学联系。表达、转录稳定性、蛋白质翻译和翻译后修饰。核心将将重点技术（研究感兴趣的一种特定产品）与大规模分析相结合基因和蛋白质水平。基因组学/蛋白质组学核心将为不同的样品处理项目，而样品本身的制备将是来自不同项目和核心 B（生理学）的研究人员的责任。一旦处理样本后，原始数据将传输给这些研究人员和核心 D（生物统计学）谁将负责数据分析和结果提取。与基因相关的其他技术需要固定样本的蛋白质表达将与 Core E 合作完成（病理）。