CHARACTERIZATION OF TRICHOMONAD LIPOPHOSPHOGLYCAN

毛滴虫脂磷酸聚糖的表征

• 批准号: 7602054
• 负责人: BIBHUTI N. SINGH
• 金额: $ 2.05万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-03 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602054
• 关键词: 6-bromo-2-naphthyl sulfate; Acids; Biological; Cattle; Computer Retrieval of Information on Scientific Projects Database; Data; Enzymes; Epithelial Cells; Fetus; Funding; Grant; Human; Institution; Investigation; Manuscripts; Natural graphite; Oligosaccharides; Parasites; Phospholipase C; Publications; Research; Research Personnel; Resources; Sexually Transmitted Diseases; Source; Structure; Trichomonas vaginalis; Trifluoroacetic Acid; United States National Institutes of Health; Up-Regulation; beta-Galactosidase; cytokine; human female; keratan-sulfate endo-1,4-b-galactosidase; lipophosphonoglycan; mass spectrometer; reproductive; sugar

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Trichomonad parasites, T. vaginalis (Tv) and T. foetus (Tf), cause major sexually transmitted diseases in human and cattle, respectively. BNS et al. purified lipophosphoglycan from both parasites an treated them with mild acid (trifluoroacetic acid) and different enzymes (endo-beta-galactosidase, another beta-galactosidase, phospholipase C) in order to obtain oligosaccharide structures short enough for MS/MS characterization. The fractions from Tv were initially characterized by MALDI-TOF MS, then the oligosaccharide portions were reduced and permethylated before analysis by LC-MS/MS, using a graphite column and a QoTOF mass spectrometer. A variety of sugars assigned to different structural motifs was identified. The data interpretation and investigation is ongoing. Biological characterization has shown that the T. vaginalis Lipophosphoglycan triggers a selective upregulation of cytokines by human female reproductive tract epithelial cells. A manuscript describing these results has been submitted for publication.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 毛滴虫寄生虫，阴道毛滴虫（Tv）和胎儿毛滴虫（Tf），分别引起人类和牛的主要性传播疾病。 BNS 等人。从两种寄生虫中纯化脂磷酸聚糖，并用弱酸（三氟乙酸）和不同的酶（内切 β-半乳糖苷酶、另一种 β-半乳糖苷酶、磷脂酶 C）对其进行处理，以获得足够短的寡糖结构，用于 MS/MS 表征。 Tv 的级分最初通过 MALDI-TOF MS 进行表征，然后将寡糖部分还原并全甲基化，然后使用石墨柱和 QoTOF 质谱仪通过 LC-MS/MS 进行分析。鉴定出分配给不同结构基序的多种糖。数据解释和调查正在进行中。生物学特性表明，阴道毛滴虫脂磷酸聚糖可触发人类女性生殖道上皮细胞选择性上调细胞因子。描述这些结果的手稿已提交出版。