STRUCTURAL STUDIES OF THE REACTION CENTER FROM RHODOBACTER SPHAEROIDES

球状红细菌反应中心的结构研究

• 批准号: 7597978
• 负责人: GEORGE FEHER
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7597978
• 关键词: Affect; Bacteriochlorophylls; Binding; Charge; Complex; Computer Retrieval of Information on Scientific Projects Database; Cytochromes c2; Electron Transport; Energy Transfer; Event; Fibrin fragment D; Funding; Grant; Harvest; Institution; Integral Membrane Protein; Light; Photosynthetic Reaction Centers; Pigments; Proteins; Protons; Quinones; Reaction; Research; Research Personnel; Resolution; Resources; Rhodobacter sphaeroides; Source; Structure; United States National Institutes of Health; Water; Work; benzoquinone; mutant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Photosynthetic reaction centers (RC) are integral membrane protein-pigment complexes that perform light induced electron transfer from a bacteriochlorophyll dimer (D) to two quinone molecules QA and QB. The RC forms a complex with another pigment-containing integral membrane protein called the light-harvesting complex (LH1) whose primary function is to gather light and transfer this energy to the RC and photo-oxidize D. In addition, a 14-kilodalton water soluble-cytochrome c2 transiently binds to the RC and re-reduces D. Our ongoing work at SSRL will focus on three structure-function aspects of the RC: 1). Determination of the structure of the RC complexed with other proteins: a). RC and light harvesting protein (LH1), and b). mutants that affect the binding and electron transfer between RC with cyt c2 . 2). Determination of the structures of mutants that affect proton and electron transfer events in the RC. 3). Determination of the structures at an enhanced resolution of intermediates that are involved in the charge separation, including D+QA-, D+QB-, and the biradical intermediate state DQA-QB-.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 光合反应中心 (RC) 是完整的膜蛋白-色素复合物，可进行从细菌叶绿素二聚体 (D) 到两个醌分子 QA 和 QB 的光诱导电子转移。 RC 与另一种含有色素的整合膜蛋白（称为光捕获复合物 (LH1)）形成复合物，其主要功能是收集光并将该能量转移到 RC 并光氧化 D。此外，14 千道尔顿的水可溶性细胞色素 c2 短暂地与 RC 结合并重新还原 D。我们在 SSRL 正在进行的工作将重点关注 RC 的三个结构功能方面：1)。与其他蛋白质复合的 RC 结构的测定：a)。 RC 和光捕获蛋白 (LH1)，以及 b)。影响 RC 与细胞色素 c2 之间的结合和电子转移的突变体。 2）。确定影响 RC 中质子和电子转移事件的突变体结构。 3）。以增强的分辨率测定参与电荷分离的中间体的结构，包括 D+QA-、D+QB- 和双自由基中间态 DQA-QB-。