Minnesota CHEAR Exposure Assessment Hub

明尼苏达州 CEAR 暴露评估中心

• 批准号: 9563479
• 负责人: STEPHEN S HECHT
• 金额: $ 98.01万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2019-09-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9563479
• 关键词: Affect; Analytical Chemistry; Area; Biological; Biological Markers; Carcinogens; Chemicals; Child; Child health care; Childhood; Clinical Research; DNA Adducts; DNA analysis; Data; Development; Diet; Disease; Environmental Carcinogens; Epigenetic Process; Exposure to; Goals; Hair; Health; Herbicides; Human; Inflammation; Insecticides; International; Life Style; Link; Mass Spectrum Analysis; Methods; Minnesota; Modification; Monitor; Organophosphates; Pesticides; Procedures; Quality Control; Research Infrastructure; Research Personnel; Resolution; Resource Development; Resources; Sampling; Scientist; Serum; Serum Albumin; Talents; Time; Tobacco; Toxic Environmental Substances; United States; Universities; Urine; Variant; adduct; base; epidemiology study; experience; metabolomics; oxidative damage; prevent; public health relevance; response; toxicant

 DESCRIPTION (provided by applicant): The Minnesota CHEAR Exposure Assessment Hub brings together highly talented investigators from the University of Minnesota and the Minnesota Department of Health with broad and internationally recognized expertise in the identification and analysis of agents that are potentially harmful to human health, including children's health. Our team is unique because our scientists have years of experience in the development and application of state-of-the-art high throughput analytical chemistry methods based mainly on mass spectrometry for the assessment of trace amounts of targeted toxicant or carcinogen metabolites in large clinical and epidemiological studies, with all appropriate sample tracking and quality control methods in place. They have also developed and applied methods for the untargeted analysis of human serum, urine, or fecal samples as well as the analysis of DNA adducts and other biological response parameters in samples from humans. Therefore, we can provide children's health researchers with access to state-of-the-art infrastructure for both targeted and untargeted analysis of biological samples as well as characterization of biological responses associated with those exposures. This goal is firmly embedded within the concept of the exposome and its relationship to children's health. The resources and cores within this Hub are as follows: The Targeted Analysis Resource will use predominantly high throughput mass spectrometric methods with well-established quality control procedures to provide timely and reliable quantitative data relevant to 3 major areas of potential importance with respect to children's health: exposure to general environmental toxicants and carcinogens; exposure to tobacco-specific compounds; and variations in levels of endogenous and dietary compounds. The Untargeted Analysis Resource will conduct high resolution mass spectrometry-based untargeted metabolomics to examine the chemical composition of diverse biological samples relevant to children's health. The Biological Response Indicators Resource will conduct state-of-the-art analyses of carcinogen-DNA adducts, biomarkers of oxidative damage including DNA adducts, biomarkers of inflammation, and parameters of epigenetic modification. The Developmental Core will develop new high throughput methods to quantify organophosphate pesticides, herbicides, and insecticides in urine as well as their adducts to serum albumin, and will establish a multi- residue approach to bio-monitor relevant chemicals in hair. The Administrative Core will coordinate all activities within the Minnesota CHEAR Exposure Assessment Hub and serve as the link between our Hub and the CHEAR Coordinating Center.

 描述（由适用提供）：明尼苏达州Chear曝光评估中心将明尼苏达大学和明尼苏达州卫生系的高度才华横溢的调查员汇集了广泛而国际认可的专业知识，以识别和分析对包括儿童健康在内的人类健康有害的代理商的识别和分析。我们的团队之所以独一无二，是因为我们的科学家在最先进的高通量分析化学方法的开发和应用方面具有多年的经验，主要基于质谱，主要基于大型临床和流行病学研究的痕量靶向毒物或致癌代谢物评估痕量量，并提供所有适当的样品跟踪和质量控制方法。他们还开发了和应用方法，用于对人血清，尿液或粪便样品的非靶向分析，以及对人类样本中的DNA加合物和其他生物反应参数的分析。因此，我们可以为儿童的健康研究人员提供对生物样本的靶向和未定式分析以及与这些暴露相关的生物学反应的表征的最先进的基础设施。该目标首先嵌入到暴露的概念及其与儿童健康的关系中。该枢纽内的资源和核心如下：目标分析资源将主要使用具有良好质量控制程序的吞吐量质谱法，以提供及时可靠的定量数据，与与儿童健康相关的3个潜在重要性相关的主要领域：暴露于一般环境有毒物质和癌症；暴露于烟草特异性化合物；内源性和饮食化合物水平的变化。未靶向的分析资源将进行高分辨率的质谱基于非靶向代谢组学，以检查潜水员生物样品与儿童健康相关的化学组成。生物响应指标资源将对致癌物中加合物，氧化损伤的生物标志物进行最先进的分析，包括DNA加合物，炎症的生物标志物以及表观遗传修饰的参数。发育核心将开发出新的高通量方法，以量化尿液中的有机磷酸盐农药，除草剂和杀虫剂及其对血清白蛋白的加合物，并将建立一种多用沉重的方法，以实现生物监测剂相关的头发中的化学物质。行政核心将协调明尼苏达州Chear暴露评估中心内的所有活动，并作为我们的枢纽与Chear协调中心之间的联系。