Targeted Analysis Resource

有针对性的分析资源

• 批准号: 10178022
• 负责人: STEPHEN S HECHT
• 金额: $ 40.11万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10178022
• 关键词: 2-Amino-1-Methyl-6-Phenylimidazo[4,5-b]pyridine; 2-butenal; 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol; 8-hydroxy-2' -deoxyguanosine; Acetaldehyde; Acetylcysteine; Acrolein; Acrylonitrile; Analytical Chemistry; Area; Aromatic Polycyclic Hydrocarbons; Ascorbic Acid; Benzene; Biological Assay; Biological Markers; Blood; Carcinogens; Carotene; Clinical Research; Communities; Cotinine; DNA; DNA Adducts; Data; Designer Drugs; Electronic cigarette; Ethylene Oxide; Exposure to; Formaldehyde; Formalin; Furans; Gas Chromatography; Glucuronides; Hair; Health; Human; Isoprostanes; Laboratories; Leukocytes; Life Style; Liquid Chromatography; Mass Fragmentography; Mass Spectrum Analysis; Metals; Methods; Minnesota; Nicotine; Nutritional; Opioid; Oxides; Paraffin Embedding; Parents; Phase; Plasma; Preparation; Proteins; Public Health; Quinoxalines; Research; Research Personnel; Resolution; Resources; Sampling; Serum; Solid; Spottings; Stable Isotope Labeling; Structure; Structure of nail of toe; Time; Tissue Embedding; Tissues; Tobacco; Tobacco-Associated Carcinogen; Tocopherols; Universities; Urine; Variant; Vitamin D; adduct; cannabinoid drug; carcinogenicity; dietary; environmental chemical exposure; epidemiology study; heterocyclic aromatic amines; hydroxycotinine; ionization; lycopene; phenanthrene; polyphenol; programs; pyridine; tandem mass spectrometry; tobacco exposure; tobacco products; toxicant; urinary

PROJECT SUMMARY/ABSTRACT: TARGETED ANALYSIS RESOURCE The Minnesota HHEAR Targeted Analysis Resource will combine the vast analytical chemistry expertise of investigators at the University of Minnesota and the Public Health Laboratory, Minnesota Department of Health. This Targeted Analysis Resource will serve the research community by providing state-of-the art quantitative analyses of biomarkers directly indicative of environmental chemical exposures including parent compounds, metabolites, and DNA or protein adducts. This Targeted Analysis Resource builds on the established methods and expertise that have been applied successfully in the Minnesota Targeted Analysis Resource of the CHEAR program. The Targeted Analysis Resource will provide timely and reliable quantitative data in three major areas of environmental and lifestyle exposures highly relevant to human health: 1. Exposure to tobacco-specific compounds; 2. Exposure to environmental, lifestyle, and nutritional toxicants and cacinogens; 3. Variations in levels of endogenous and dietary compounds. The tobacco-specific compounds are critical for establishing exposure to tobacco products or e-cigarettes. They include the full suite of urinary nicotine metabolites (nicotine, cotinine, 3′-hydroxycotinine and their glucuronides, and nicotine-N-oxide); cotinine and 3′-hydroxycotinine in serum, dried blood spots, and toenails; and the tobacco-specific carcinogen metabolites 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and its glucuronides in urine, blood, and toenails. The environmental, lifestyle, and nutritional toxicants and carcinogens and metabolites include mercapturic acid (MA) metabolites of volatile toxicants such as acrylonitrile (CEMA), benzene (SPMA), acrolein (3-HPMA), crotonaldehyde (HMPMA), and ethylene oxide (HEMA); urinary metabolites of furan; urinary metabolites of polycyclic aromatic hydrocarbons (PAH) such as 1-hydroxypyrene (1-HOP) and phenanthrene tetraol (PheT); urinary metals; per- and polyfluoroalkyl substances; opioids, cannabinoids, and designer drugs; leukocyte DNA adducts of formaldehyde, acetaldehyde, and acrolein; and DNA adducts of heterocyclic aromatic amines in formalin fixed paraffin embedded tissues. The endogenous and dietary compounds include the carcinogenic urinary heterocyclic aromatic amines 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) and 2-amino-1-methyl-6- pheynylimidazo[4,5-b]pyridine (PhIP) and their metabolites; PhIP in hair; tissue 8-hydroxydeoxyguanosine; plasma carotenes, lycopenes, tocopherols, ascorbic acid, vitamin D derivatives, isoprostanes, and urinary polyphenols.

项目摘要/摘要：目标分析资源 明尼苏达州 HHEAR 目标分析资源将结合广泛的分析化学专业知识 明尼苏达大学和明尼苏达州公共卫生实验室的研究人员 健康。这一有针对性的分析资源将通过提供最先进的技术为研究界服务。 直接指示环境化学暴露（包括母体）的定量生物标志物分析 该目标分析资源以化合物、代谢物和 DNA 或蛋白质加合物为基础。 已在明尼苏达目标分析中成功应用的既定方法和专业知识 CEAR 计划的资源将提供及时且可靠的定量信息。 环境和生活方式暴露与人类健康高度相关的三个主要领域的数据： 1. 接触烟草特有的化合物； 2. 接触环境、生活方式和营养毒物和致癌物； 3.内源性和膳食化合物水平的变化。 烟草特有的化合物对于确定接触烟草产品或电子烟至关重要。 它们包括全套尿尼古丁代谢物（尼古丁、可替宁、3'-羟基可替宁及其 血清、干血斑和脚趾甲中的葡萄糖醛酸苷和尼古丁-N-氧化物）； 以及烟草特有的致癌代谢物4-(甲基亚硝基氨基)-1-(3-吡啶基)-1-丁醇(NNAL)及其 尿液、血液和脚趾甲中的葡萄糖醛酸苷 环境、生活方式和营养毒物和 致癌物和代谢物包括挥发性毒物的硫醇酸（MA）代谢物，例如 丙烯腈 (CEMA)、苯 (SPMA)、丙烯醛 (3-HPMA)、巴豆醛 (HMPMA) 和环氧乙烷 (HEMA)；呋喃的尿代谢物；多环芳烃(PAH)的尿代谢物； 1-羟基芘（1-HOP）和菲四醇（PheT）；全氟烷基和多氟烷基； 阿片类物质、大麻素和甲醛的白细胞 DNA 加合物； 乙醛和丙烯醛；以及福尔马林固定石蜡中的杂环芳香胺的 DNA 加合物 内源性和膳食化合物包括致癌的尿杂环。 芳香胺 2-氨基-3,8-二甲基咪唑并[4,5-f]喹喔啉 (MeIQx) 和 2-氨基-1-甲基-6- 毛发组织中的苯咪唑并[4,5-b]吡啶（PhIP）及其代谢物； 血浆胡萝卜素、番茄红素、生育酚、抗坏血酸、维生素 D 衍生物、异前列腺素和尿 多酚。