Targeted Analysis Resource

有针对性的分析资源

• 批准号: 10178022
• 负责人: STEPHEN S HECHT
• 金额: $ 40.11万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10178022
• 关键词: 2-Amino-1-Methyl-6-Phenylimidazo[4,5-b]pyridine; 2-butenal; 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol; 8-hydroxy-2' -deoxyguanosine; Acetaldehyde; Acetylcysteine; Acrolein; Acrylonitrile; Analytical Chemistry; Area; Aromatic Polycyclic Hydrocarbons; Ascorbic Acid; Benzene; Biological Assay; Biological Markers; Blood; Carcinogens; Carotene; Clinical Research; Communities; Cotinine; DNA; DNA Adducts; Data; Designer Drugs; Electronic cigarette; Ethylene Oxide; Exposure to; Formaldehyde; Formalin; Furans; Gas Chromatography; Glucuronides; Hair; Health; Human; Isoprostanes; Laboratories; Leukocytes; Life Style; Liquid Chromatography; Mass Fragmentography; Mass Spectrum Analysis; Metals; Methods; Minnesota; Nicotine; Nutritional; Opioid; Oxides; Paraffin Embedding; Parents; Phase; Plasma; Preparation; Proteins; Public Health; Quinoxalines; Research; Research Personnel; Resolution; Resources; Sampling; Serum; Solid; Spottings; Stable Isotope Labeling; Structure; Structure of nail of toe; Time; Tissue Embedding; Tissues; Tobacco; Tobacco-Associated Carcinogen; Tocopherols; Universities; Urine; Variant; Vitamin D; adduct; cannabinoid drug; carcinogenicity; dietary; environmental chemical exposure; epidemiology study; heterocyclic aromatic amines; hydroxycotinine; ionization; lycopene; phenanthrene; polyphenol; programs; pyridine; tandem mass spectrometry; tobacco exposure; tobacco products; toxicant; urinary

PROJECT SUMMARY/ABSTRACT: TARGETED ANALYSIS RESOURCE The Minnesota HHEAR Targeted Analysis Resource will combine the vast analytical chemistry expertise of investigators at the University of Minnesota and the Public Health Laboratory, Minnesota Department of Health. This Targeted Analysis Resource will serve the research community by providing state-of-the art quantitative analyses of biomarkers directly indicative of environmental chemical exposures including parent compounds, metabolites, and DNA or protein adducts. This Targeted Analysis Resource builds on the established methods and expertise that have been applied successfully in the Minnesota Targeted Analysis Resource of the CHEAR program. The Targeted Analysis Resource will provide timely and reliable quantitative data in three major areas of environmental and lifestyle exposures highly relevant to human health: 1. Exposure to tobacco-specific compounds; 2. Exposure to environmental, lifestyle, and nutritional toxicants and cacinogens; 3. Variations in levels of endogenous and dietary compounds. The tobacco-specific compounds are critical for establishing exposure to tobacco products or e-cigarettes. They include the full suite of urinary nicotine metabolites (nicotine, cotinine, 3′-hydroxycotinine and their glucuronides, and nicotine-N-oxide); cotinine and 3′-hydroxycotinine in serum, dried blood spots, and toenails; and the tobacco-specific carcinogen metabolites 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and its glucuronides in urine, blood, and toenails. The environmental, lifestyle, and nutritional toxicants and carcinogens and metabolites include mercapturic acid (MA) metabolites of volatile toxicants such as acrylonitrile (CEMA), benzene (SPMA), acrolein (3-HPMA), crotonaldehyde (HMPMA), and ethylene oxide (HEMA); urinary metabolites of furan; urinary metabolites of polycyclic aromatic hydrocarbons (PAH) such as 1-hydroxypyrene (1-HOP) and phenanthrene tetraol (PheT); urinary metals; per- and polyfluoroalkyl substances; opioids, cannabinoids, and designer drugs; leukocyte DNA adducts of formaldehyde, acetaldehyde, and acrolein; and DNA adducts of heterocyclic aromatic amines in formalin fixed paraffin embedded tissues. The endogenous and dietary compounds include the carcinogenic urinary heterocyclic aromatic amines 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) and 2-amino-1-methyl-6- pheynylimidazo[4,5-b]pyridine (PhIP) and their metabolites; PhIP in hair; tissue 8-hydroxydeoxyguanosine; plasma carotenes, lycopenes, tocopherols, ascorbic acid, vitamin D derivatives, isoprostanes, and urinary polyphenols.

项目摘要/摘要：目标分析资源 明尼苏达州HHEAR针对分析资源将结合大量的分析化学专家 明尼苏达大学的调查人员和明尼苏达州公共卫生实验室 健康。这个有针对性的分析资源将通过提供最先进的技术来为研究社区提供服务 对生物标志物的定量分析直接指示环境化学暴露在内 化合物，代谢产物以及DNA或蛋白质添加剂。这个针对性的分析资源建立在 已成功应用于明尼苏达州的目标分析的既定方法和专业知识 Chear计划的资源。目标分析资源将提供及时可靠的定量 环境和生活方式暴露的三个主要领域的数据与人类健康高度相关： 1。暴露于烟草特异性化合物； 2。暴露于环境，生活方式，营养有毒物质和cacinogens； 3。内源性和饮食化合物水平的变化。 烟草特异性化合物对于建立暴露于烟草产品或电子烟至关重要。 它们包括完整的尿液尼古丁代谢物（尼古丁，可替宁，3'-羟基霉素及其 谷氨酸苷和尼古丁-N-氧化物）；可替宁和3'-羟基霉素，血清干燥的血斑和脚趾甲； 和烟草特异性的致癌代谢物4-（甲基硝基氨基）-1-（3-吡啶基）-1-丁醇（Nnal）及其 尿液，血液和脚趾甲中的葡萄糖醛酸化物。环境，生活方式和营养有毒物质和 致癌物和代谢产物包括挥发性有毒物质（例如 丙烯腈（CEMA），苯（SPMA），丙烯醛（3-HPMA），Crotonaldehyde（HMPMA）和氧化乙烷氧化物 （hema）; Furan的尿代谢物；多环芳烃（PAH）的尿代谢物，例如 1-羟基苯乙烯（1-HOP）和菲四酚（PHET）；泌尿金属；每个氟烷基和多氟烷基 物质；阿片类药物，大麻素和设计师药物；甲醛的白细胞DNA加合物， 乙醛和丙烯醛；福尔马林固定石蜡中杂环芳香胺的DNA加合物 嵌入组织。内源性和饮食化合物包括致癌性泌尿杂环 芳香胺2-氨基-3,8-二甲基咪唑[4,5-F] quinoxaline（MeiQX）和2-Amino-1-甲基-6-- Pheynylimidazo [4,5-B]吡啶（PHIP）及其代谢产物；头发的phip;组织8-羟基氧鸟苷； 血浆胡萝卜素，番茄酚，抗坏血酸，维生素D衍生物，异丙烷和尿液 多酚。