Studies Of Gastroenteritis Viruses By Electron Microscopy

通过电子显微镜研究胃肠炎病毒

• 批准号: 8555748
• 负责人: Albert Z Kapikian
• 金额: $ 2.64万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8555748
• 关键词: Annual Reports; Astrovirus; Calicivirus; Cell Culture System; Characteristics; Child; Classification; Clinical Research; Collaborations; Coronavirus; Detection; Diarrhea; Disease; Electron Microscope; Electron Microscopy; Enteral; Epidemiologic Studies; Epidemiology; Etiology; Family; Feces; Gastroenteritis; Goals; Gold; Hepatitis A Virus; Hepatitis E virus; Hepatitis Viruses; Hospitalized Child; Human; Imagery; Immune; Infant; Infantile Diarrhea; Infectious hepatitides; Investigation; Laboratory Study; Natural History; Norovirus; Norwalk virus; Play; Research; Role; Rotavirus; Specimen; System; Techniques; Torovirus; Viral Gastroenteritis; Virion; Virus; Virus-like particle; enteric adenovirus infection; member; programs; tissue culture; tool

For over three decades, the electron microscope has been an integral part of the research program of the Epidemiology Section regarding the etiologic role, natural history and physical characteristics of various important enteric viruses. Past highlights of these investigations have included inquiry into the etiology of diseases of unknown cause involving studies of fastidious agents of disease that defied cultivation in any cell culture system. The electron microscope played a major role (i) in the discovery of the 27nm Norwalk virus (now known as a member of the norovirus genus in the calicivirus family) and the establishment of its etiologic role in human diarrheal disease, and (ii) in collaboration with the Hepatitis Viruses Section (HVS), in the discovery of the 27nm hepatitis A virus particle and establishment of its etiologic role in infectious hepatitis and in addition in studies of the hepatitis E virus with the HVS . It also was used as an important epidemiologic tool for the detection of rotaviruses in stools of infants and young children hospitalized with diarrhea and helped in establishing the importance of these agents in severe diarrhea of infants and young children. Although intensive efforts have been made, the Norwalk and related human enteric noroviruses have yet to be cultivated in any tissue culture system. This, along with the visualization of virus-like particles (VLPs) of certain members of this group of agents, have contributed to the continuing important role of the electron microscope in the research program of the LID. Although the electron microscope had limited use since the previous annual report, the electron microscope will continue to be an important primary and adjunctive tool in various capacities. Since last year's annual report, it was used by Dr. Green to demonstrate that numerous VLPs were present in a norovirus construct (NV-2007) prepared by Dr.Bok.

在过去的三十年中，电子显微镜一直是有关各种重要肠道病毒的病因学作用，自然历史和物理特征的流行病学部分研究计划的组成部分。这些研究的过去亮点包括对未知原因疾病的病因进行调查，这些病因涉及对任何细胞培养系统中违反培养的疾病的研究。电子显微镜在发现27nm Norwalk病毒（现在被称为钙病毒家族中诺如病毒属的成员）中起着重要作用（i），并在人类腹泻病中建立了病因学作用，以及（ii）与肝炎病毒病毒的合作（II），在肝炎病毒部分的合作（HVS）中的发现（Hepaties）在感染性肝炎中的作用以及HVS的丙型肝炎病毒的研究。它也被用作重要的流行病学工具，用于检测患有腹泻住院的婴儿和幼儿的轮状病毒，并帮助确立了这些药物在婴儿和幼儿严重腹泻中的重要性。尽管已经做出了密集的努力，但在任何组织培养系统中尚未培养诺瓦克和相关的人类北病毒。这加上该群体某些成员的病毒样颗粒（VLP）的可视化，也有助于电子显微镜在盖子研究计划中持续重要的重要作用。尽管自上一年报告以来，电子显微镜的用途有限，但电子显微镜将继续是各种能力的重要主要和辅助工具。自去年的年度报告以来，格林博士使用它证明了Bok Dr. Bok Dr. Brok Dr. Bok Dr. Broke构造（NV-2007）中存在许多VLP。