Molecular epidemiological study of diarrheal viruses (international comparison)

腹泻病毒分子流行病学研究（国际比较）

基本信息

批准号：
10044241
负责人：
USHIJIMA Hiroshi
金额：
$ 2.43万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044241/
关键词：
diarrheal viruses molecular epidemiology international rotavirus calicivirus adenovirus astrovirus 酵素抗体法 RT-PCR

项目摘要

Infantile morality due to diarrheal viruses is important problem in developing countries. Early diagnosis and treatment are desired. Molecular epidemiological study is essential to know emerging and re-emerging of the viruses. (1) Latex reagent was developed on astrovirus (AV) to diagnose clinical stool samples. Genotype (serotype) specific primers of AV-RT-PCR were designed and used for the samples. AV serotype I was predominant in developing countries while AV serotype 5-epidemic was recognized in Beijing. (2) Rotavirus (RV) serotype 1 has been major type in Asia except Soul, however RV serotype 9 has been increasing. The predominant months are Feb and March in recent years although it was Dec and Jan before. Even in a same serotype, it could be devided into subtypes. Reassortant RV was discovered in China. (3) Adenovirus could be diagnoed by PCR in addition serotype was determined with restriction enzyme on PCR products. (4) Calicivirus was diagnosed by RT-PCR. Almost all positive samples were G2. Calicivirus was predominant in Nov and Dec. (5) Anti-rotavirus agents were discovered in sulfated colominic acid and cacao pigment. (6) RV vaccine is necessary especially in developing countries. RV vaccine is recognized as cost effective in this research also.These studies were conducted with researchers in Beijing, Chiang Mai, Soul, Taipei, Mainz, Oslo and Japan.

腹泻病毒引起的婴儿道德问题是发展中国家的重要问题。需要早期诊断和治疗。分子流行病学研究对于了解病毒的出现和重新出现至关重要。 (1)针对星状病毒(AV)开发乳胶试剂，用于诊断临床粪便样本。设计了 AV-RT-PCR 基因型（血清型）特异性引物并将其用于样品。 AV 血清型 I 在发展中国家占主导地位，而 AV 血清型 5 型在北京流行。 (2) 轮状病毒(RV)血清型1是亚洲除Soul外的主要病毒型，但RV血清型9一直在增加。尽管以前是 12 月和 1 月，但近年来主要月份是 2 月和 3 月。即使在同一血清型中，也可以分为亚型。重组房车在中国被发现。 (3)通过PCR可诊断腺病毒，并对PCR产物进行限制性内切酶测定血清型。 (4)RT-PCR诊断杯状病毒。几乎所有阳性样本都是G2。 11月和12月以杯状病毒为主。 (5)在硫酸化多聚乙醇酸和可可色素中发现抗轮状病毒剂。 (6) RV疫苗特别是在发展中国家是必要的。在这项研究中，RV 疫苗也被认为具有成本效益。这些研究是与北京、清迈、苏尔、台北、美因茨、奥斯陆和日本的研究人员共同进行的。