Basic and clinical research of viral gastroenteritis in children and its risk evaluation

儿童病毒性胃肠炎的基础、临床研究及风险评估

• 批准号: 21390315
• 负责人: USHIJIMA Hiroshi
• 金额: $ 11.81万
• 依托单位: Nihon University (2011)Aino University (2009-2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390315/
• 关键词: ウイルス; 胃腸炎; 小児; 分子疫学; リスク評価

We have produced new virus-like particles(VLPs) of norovirus and sapovirus in order to update the consequences of viral genetic variability. The immunochromatography(IC) diagnosis test kits have been developed/improved by these new VLPs. In addition to the molecular epidemiological study of rotavirus, norovirus, sapovirus, adenovirus and astrovirus that we have done, the novel diarrheal viruses such as Aichi virus, parechovirus, bocavirus and Saffold virus have also been found in diarrheal stool samples. Murine norovirus was used as a novel surrogate to evaluate disinfectants, heating, and antiviral activity for human norovirus. We challenged multiple-cell cultivation assays for norovirus but did not succeed at this moment. We also examined the binding activities of norovirus and sapovirus to histo-blood group antigens of saliva and intestinal cells. It was interesting to find that norovirus, but not sapovirus, showed high binding activity.

我们生产了诺如病毒和沙波病毒的新病毒样颗粒（VLP），以更新病毒遗传变异的后果。这些新的VLP已经开发/改进了免疫层析（IC）诊断检测试剂盒。除了我们所做的轮状病毒、诺如病毒、沙波病毒、腺病毒和星状病毒的分子流行病学研究外，还在腹泻粪便样本中发现了爱知病毒、副肠病毒、博卡病毒和萨福尔德病毒等新型腹泻病毒。鼠诺如病毒被用作新型替代物来评估人诺如病毒的消毒剂、加热和抗病毒活性。我们挑战了诺如病毒的多细胞培养测定，但目前没有成功。我们还检查了诺如病毒和沙波病毒与唾液和肠道细胞的组织血型抗原的结合活性。有趣的是，诺如病毒（而非沙波病毒）表现出高结合活性。

项目成果

Sequence analysis of the capsid gene of Aichi viruses detected from Japan, Bangladesh, Thailand, and Vietnam

• DOI: 10.1002/jmv.21193
• 发表时间: 2008-07
• 期刊: Journal of Medical Virology
• 影响因子: 12.7
• 作者: N. Pham;Q. D. Trinh;P. Khamrin;T. A. Nguyen;S. Dey;T. Phan;L. Hoang;N. Maneekarn;S. Okitsu;M. Mizuguchi;H. Ushijima

ロタウイルス胃腸炎の予防と治療の新しい展開

轮状病毒胃肠炎防治新进展

• 发表时间: 2011
• 作者: Ohnishi M;Kyo A;Bright A;Leshabari S.;牛島廣治
• 通讯作者: 牛島廣治

Viral Molecular Characterization of the First Congenital Rubella Syndrome Case in Vietnam

越南首例先天性风疹综合征病例的病毒分子特征

• 发表时间: 2011
• 期刊: Clin Lab
• 影响因子: 0.7
• 作者: Kaburaki T;et al;Tran DN
• 通讯作者: Tran DN

Molecular epidemiology of human and animal kobuvirus

人类和动物科布病毒的分子流行病学

• 发表时间: 2011
• 期刊: 臨床とウイルス
• 作者: Suzuki Y;Ito Y;Mizuno M;Kinashi H;Sawai A;Noda Y;Mizuno T;Shimizu H;Fujita Y;Matsui K;Maruyama S;Imai E;Matsuo S;Takei Y;Khamrin P
• 通讯作者: Khamrin P

Exceptionally high prevalence of porcine kobuviruses in piglets with diarrhea

腹泻仔猪中猪科布病毒的患病率异常高

• 发表时间: 2009
• 作者: カムリン・パタラ;et al.
• 通讯作者: et al.