Autoimmunity Lymphocyte Repertoire Core (ALRC)

自身免疫淋巴细胞库核心 (ALRC)

基本信息

批准号：
7688953
负责人：
Patrick Christopher Wilson
金额：
$ 21.88万
依托单位：
UNIVERSITY OF CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-06-10 至 2014-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7688953
关键词：
Antibodies Antibody Formation Antibody Specificity Antigens Arts Autoantibodies Autoimmune Diseases Autoimmune Process Autoimmunity B cell repertoire B-Lymphocytes Binding Biology Cell Count Cells Chicago Clinical Trials Clonality Cloning Data Evaluation Flow Cytometry Generalized Myasthenia Gravis Generations Genes Genetic Materials Human Hybridomas Immunity Immunoglobulins Invaded Kidney Laboratories Lead Learning Light Light-Chain Immunoglobulins Lupus Lymphocyte Measures Mediating Methodology Microarray Analysis Monoclonal Antibodies Mus Myasthenia Gravis Neuromyelitis Optica Oncogenic Viruses Paper Pathology Patients Phenotype Pilot Projects Population Process Publishing Recombinant Antibody Recombinants Research Personnel Research Project Grants Resistance Role Services Somatic Mutation Sorting - Cell Movement Source Specificity System Systemic Lupus Erythematosus Technology Testing Universities Virus Diseases aquaporin 4 authority autoreactive B cell autoreactivity design insight member neoplastic new technology peripheral blood receptor rituximab tool

项目摘要

This core will provide services to University of Chicago Autoimmunity Center of Excellence (UCACE) investigators to characterize the role of B lymphocytes in autoimmune pathology. There are two state-of-the-art technologies that will allow the center investigators to scrutinize autoantibody-mediated pathology. 1. Analysis of B lymphocyte specificity using recombinant monoclonal (mAb) technology. 2. Immunoglobulin light chain variable gene microarrays Finally, Drs. Weigert and Wilson will assist and provide technical support for studies of the immunoglobulin repertoire using single-cell PCR, cloning and sequencing. This approach will augment the use of light chain microarrays and human mAb analysis. The unique methodologies for scrutinizing human B cell mediated pathology will provide powerful tools to the center to facilitate our understanding of lupus and myasthenia gravis.

该核心将为芝加哥大学自身免疫卓越中心（UCACE）提供服务研究人员描述了 B 淋巴细胞在自身免疫病理学中的作用。有两个最先进的使中心研究人员能够仔细检查自身抗体介导的病理学的技术。 1. 使用重组单克隆（mAb）技术分析B淋巴细胞特异性。 2. 免疫球蛋白轻链可变基因微阵列最后，博士。 Weigert和Wilson将为免疫球蛋白的研究提供协助和技术支持使用单细胞 PCR、克隆和测序来构建库。这种方法将增强轻链的使用微阵列和人类单克隆抗体分析。用于检查人类 B 细胞介导的病理学的独特方法将为中心以促进我们对狼疮和重症肌无力的了解。