Mapping Neural Circuits Using Pseudorabie Virus Vectors

使用伪狂犬病病毒载体绘制神经回路

基本信息

批准号：
7580993
负责人：
JEFFREY M FRIEDMAN
金额：
$ 34.87万
依托单位：
ROCKEFELLER UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-02-15 至 2010-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7580993
关键词：
Amygdaloid structure Animal Viruses Animals Antibodies Bacterial Artificial Chromosomes Behavior Binding Proteins Brain Brain region Cells Complement Complex DNA Microarray Chip Data Databases Development Elements Enterobacteria phage P1 Cre recombinase Epitopes Escherichia G-Protein-Coupled Receptors Genes Genetic Genetic Transcription Green Fluorescent Proteins Hypothalamic structure Immunoprecipitation Infection Label Maps Melanocortin 4 Receptor Methods Motor Mus Neural Pathways Neurons Nucleoproteins Partner in relationship Pathway interactions Peptides Peripheral Play Population Pro-Opiomelanocortin Protein Fragment Proteins Publishing RNA Recombinants Reporter Genes Research Personnel Salivary Glands Series Site Source Structure of nucleus infundibularis hypothalami Suid Herpesvirus 1 System Testing Time Tongue Tracer Transgenic Mice Transgenic Organisms Variant Virus Virus Diseases Wheat Germ Agglutinins beta-Galactosidase cell type enzyme activity feeding interest leptin receptor neural circuit neuropeptide Y novel programs promoter protein expression recombinase red fluorescent protein retrograde transport vector

项目摘要

DESCRIPTION (provided by applicant): This proposal outlines the development of novel methods for the mapping of complex neural pathways and the specification of the neurons that compose these pathways. Our groups have recently made a recombinant Pseudorabies virus (PRV) that is conditional on cre-recombinase for viability and expression of a GFP reporter gene. This virus has been used successfully to map inputs to specific neuronal populations in defined brain regions. For simplicity we will refer to this and related methods as PAM, Pseudorabies virus assisted mapping. In this application, we propose to further develop PAM so as to allow: 1) Simultaneous Retrograde Tracing from Different Neurons in the Same Brain Region, 2) Simultaneous Retrograde Tracing from Similar Neurons in Different Brain Regions, 3) Anterograde tracing 4) Identification of Neurons Projecting to More than One Site 5) Specification of the neurons that are identified in these mapping studies. To establish the validity of the approach, these studies will be performed in analyses of hypothalamic circuits that regulate feeding. Once the methods are validated, the viruses and animals will be made freely available to other investigators interested in analogous studies of other neuronal pathways.

描述（由申请人提供）：该提案概述了绘制复杂神经通路的新方法的开发以及构成这些通路的神经元的规格。我们的团队最近制备了一种重组伪狂犬病病毒 (PRV)，其以 cre 重组酶为条件，以实现 GFP 报告基因的活力和表达。该病毒已成功用于将输入映射到特定大脑区域的特定神经元群。为简单起见，我们将此方法和相关方法称为 PAM（伪狂犬病病毒辅助作图）。在此应用中，我们建议进一步开发 PAM，以便允许：1）同一大脑区域中不同神经元的同时逆行追踪，2）不同大脑区域中相似神经元的同时逆行追踪，3）顺行追踪 4）识别投射到多个位点的神经元 5) 在这些映射研究中确定的神经元的规格。为了确定该方法的有效性，这些研究将对调节进食的下丘脑回路进行分析。一旦这些方法得到验证，病毒和动物将免费提供给对其他神经元通路的类似研究感兴趣的其他研究人员。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Molecular profiling of neurons based on connectivity.

DOI：
10.1016/j.cell.2014.03.059
发表时间：
2014-05-22
期刊：
Cell
影响因子：
64.5
作者：
Ekstrand MI;Nectow AR;Knight ZA;Latcha KN;Pomeranz LE;Friedman JM
通讯作者：
Friedman JM

Molecular annotation of integrative feeding neural circuits.

DOI：
10.1016/j.cmet.2010.12.013
发表时间：
2011-02-02
期刊：
Cell metabolism
影响因子：
29
作者：
Pérez CA;Stanley SA;Wysocki RW;Havranova J;Ahrens-Nicklas R;Onyimba F;Friedman JM
通讯作者：
Friedman JM