Mapping Neural Circuits Using Pseudorabie Virus Vectors

使用伪狂犬病病毒载体绘制神经回路

基本信息

批准号：
7339868
负责人：
JEFFREY M FRIEDMAN
金额：
$ 35.36万
依托单位：
ROCKEFELLER UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-02-15 至 2009-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7339868
关键词：
Amygdaloid structure Animal Viruses Animals Antibodies Bacterial Artificial Chromosomes Bacterial Genes Behavior Binding Proteins Brain Brain region Cells Complement Complex DNA Microarray Chip DNA Microarray format Data Databases Development Elements Enterobacteria phage P1 Cre recombinase Epitopes Escherichia G-Protein-Coupled Receptors Genes Genetic Genetic Transcription Green Fluorescent Proteins Hypothalamic structure Immunoprecipitation Infection Label Localized Maps MeSH Thesaurus Melanocortin 4 Receptor Melanocyte stimulating hormone Methods Motor Mus Neural Pathways Neurons Nucleoproteins POMC gene Partner in relationship Pathway interactions Peptides Peripheral Play Population Pro-Opiomelanocortin Protein Fragment Proteins Publishing RNA Recombinants Reporter Genes Research Personnel Salivary Glands Series Site Source Structure of nucleus infundibularis hypothalami Suid Herpesvirus 1 System Testing Time Tongue Tracer Transgenic Mice Transgenic Organisms Variant Virus Virus Diseases Wheat Germ Agglutinins beta-Galactosidase cell type day enzyme activity feeding interest leptin receptor neural circuit neuropeptide Y novel programs promoter protein expression recombinase red fluorescent protein retrograde transport vector

项目摘要

DESCRIPTION (provided by applicant): This proposal outlines the development of novel methods for the mapping of complex neural pathways and the specification of the neurons that compose these pathways. Our groups have recently made a recombinant Pseudorabies virus (PRV) that is conditional on cre-recombinase for viability and expression of a GFP reporter gene. This virus has been used successfully to map inputs to specific neuronal populations in defined brain regions. For simplicity we will refer to this and related methods as PAM, Pseudorabies virus assisted mapping. In this application, we propose to further develop PAM so as to allow: 1) Simultaneous Retrograde Tracing from Different Neurons in the Same Brain Region, 2) Simultaneous Retrograde Tracing from Similar Neurons in Different Brain Regions, 3) Anterograde tracing 4) Identification of Neurons Projecting to More than One Site 5) Specification of the neurons that are identified in these mapping studies. To establish the validity of the approach, these studies will be performed in analyses of hypothalamic circuits that regulate feeding. Once the methods are validated, the viruses and animals will be made freely available to other investigators interested in analogous studies of other neuronal pathways.

描述（由申请人提供）：该提案概述了复杂神经途径映射的新方法以及组成这些途径的神经元的规范。我们的小组最近制作了重组假性病毒（PRV），该病毒（PRV）是基于Cre-recombinase的条件，用于GFP报告基因的生存能力和表达。该病毒已成功地将输入映射到定义的大脑区域的特定神经元种群中。为简单起见，我们将这种方法和相关方法称为PAM，伪rabies病毒辅助映射。在此应用中，我们建议进一步发展PAM，以允许：1）同时从同一大脑区域的不同神经元中逆行追踪，2）同时从不同大脑区域的相似神经元中同时逆行追踪，3）前进的跟踪4）对神经元的神经元的识别5）识别，该神经元比单位5）识别了这些神经元的识别。为了确定该方法的有效性，这些研究将在调节进食的下丘脑回路的分析中进行。一旦方法得到验证，将向其他对其他神经元途径类似研究感兴趣的研究者免费获得病毒和动物。