Next Generation ALPHA Zebrafish Tank Washer: High Throughput with Reduced Environmental Impact

下一代 ALPHA 斑马鱼槽清洗机：高吞吐量，减少环境影响

基本信息

批准号：
10737523
负责人：
LILIANNA SOLNICAKREZEL
金额：
$ 22.32万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-07-01 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10737523
关键词：
Adult Animals Beds Biomechanics Breeding Carbon Chemicals Congenital Abnormality Consumption Development Disease Dose Education and Outreach Electricity Embryo Embryo Research Ensure Environmental Impact Equipment Exhibits Faculty Filtration Fishes Food Fresh Water Genome engineering Grant Heating Hour Housing Hydrogen Peroxide Laboratories Laboratory Research Life Liquid substance Maintenance Manuals Microinjections Microscope Modeling Modernization Movement Mus Operations Research Organism Osmosis Partner in relationship Pathologic Postdoctoral Fellow Productivity Quarantine Research Research Activity Research Personnel Research Support Robotics Scientist Services Source Steam System Technology Testing Time United States National Institutes of Health Universities Walking Washington Water Water Purification Zebrafish feeding fine particles genetic analysis graduate student human model improved manufacture medical schools member next generation operation outreach program particle programs prototype repaired sensor sperm cryopreservation ultraviolet irradiation undergraduate student water flow water quality zygote

项目摘要

Zebrafish is a premier research organism for the genetic analysis of vertebrate development and modeling human birth defects and disease. The Zebrafish Facility at Washington University School of Medicine in St. Louis is a shared-used facility. This 5,644 sf state-of-the-art facility accommodates a total of nearly 9,000 tanks in four animal holding rooms with independent recirculating water systems. The Facility provides optimal conditions for the rearing and maintenance of up to 200,000 fish. Starting with four zebrafish laboratories in 2011, the Facility currently supports research of 33 independent laboratories. Presently, over 100 graduate and undergraduate students, postdoctoral fellows and scientists carry out research in the Facility that is supported by R00, R01, R54, and P01 grants from the National Institutes of Health. The Facility also supports other educational and outreach programs. The Facility enables these programs by ensuring superior husbandry with automatic feeding that halves the time zebrafish grow to adulthood; supplying wild-type lines and embryos for experimentation, cryopreserving sperm to minimize the number of lines maintained as life stocks, conducting pathological testing; maintaining Quarantine System for imported lines, providing research services such as genome engineering, and washing and disinfecting holding and breeding tanks and their components to ensure safe and efficient research operations. The continued increase of the zebrafish users and expansion to 9,000 tanks requires modern, technologically advanced equipment to clean and disinfect, on a daily basis, hundreds of holding and breeding tanks, and tank components as well as fish nets to ensure safe and efficient zebrafish research. Here we propose replacing the existing 900 GP Walk in Washer (Tecniplast) with the next generation ALPHA Walk in Washer model (Tecniplast). The existing 900 GP Washer is a prototype aquatics washer with several limitations: it uses steam lines to heat water, is energetically inefficient, requires long wash cycles, uses an external chemical dosing system, and breaks down frequently. These inefficiencies and frequent downtimes increase manual labor, limiting and slowing down the Facility operations and research activities it supports. The ALPHA model deploys adaptive cleaning technology that combines independent vertical and oscillating movements allowing for superior water coverage. The ALPHA washer uses electricity to heat water, shorter wash cycles and thus higher throughput that would meet the continually growing washing needs of the zebrafish researchers. The reservoir for the chemicals used during wash cycles fit into the machine footprint. Operations with the ALPHA Washer would reduce environmental impact with a shorter wash cycle and consuming smaller amounts of chemicals per cycle. The proposed upgrade to the modern ALPHA Walk in Washer would dramatically improve the washing capacity of zebrafish holding and breeding tanks; would meet the needs of the growing zebrafish research operations and would also reduce the Facility’s environmental impact.

斑马鱼是脊椎动物发育和建模遗传分析的首要研究生物人类出生缺陷和疾病。圣路易斯华盛顿大学医学院的斑马鱼设施。这座占地 5,644 平方英尺的先进设施共容纳近 9,000 个储罐，共 4 个。具有独立循环水系统的动物饲养室为动物提供了最佳条件。该设施于 2011 年建立了四个斑马鱼实验室，可饲养和维护多达 200,000 条鱼。目前支持 33 个独立实验室的研究，目前有 100 多个研究生和本科生。学生、博士后研究员和科学家在 R00、R01、美国国立卫生研究院的 R54 和 P01 拨款还支持其他教育和研究。该设施通过确保自动饲喂的优质饲养来实现这些计划。将斑马鱼成长到成年的时间减半；提供野生型品系和胚胎用于实验，冷冻保存精子以最大程度地减少作为生命种群的品系数量，并进行病理检测；维护进口品系的检疫系统，提供基因组工程等研究服务，对暂养池、养殖池及其部件进行清洗消毒，确保安全高效研究业务。斑马鱼用户的不断增加和水箱数量的扩大需要现代化的技术先进的设备每天对数百个储存池、养殖池和水池进行清洁和消毒组件以及鱼网，以确保安全高效的斑马鱼研究。在这里，我们建议更换。现有 900 GP 步入式洗衣机 (Tecniplast) 和新一代 ALPHA 步入式洗衣机型号 (Tecniplast) 现有的 900 GP 洗衣机是一款原型水生洗衣机，有几个局限性：它使用蒸汽。管线加热水，能源效率低下，需要较长的洗涤周期，使用外部化学剂量这些低效率和频繁的停机增加了体力劳动，限制和减缓其支持的设施运营和研究活动。 ALPHA 型号采用自适应清洁技术，结合了独立的垂直和摆动 ALPHA 洗衣机使用电力来加热水，从而实现卓越的水覆盖。清洗周期，从而提高吞吐量，满足斑马鱼不断增长的清洗需求研究人员表示，清洗周期中使用的化学物质的储存器适合机器的操作。使用 ALPHA 洗衣机可缩短洗涤周期并减少消耗，从而减少对环境的影响拟议的现代 ALPHA Walk in Washer 升级将减少化学品用量。大幅提高斑马鱼饲养池和养殖池的清洗能力，满足斑马鱼饲养池的需要；增加斑马鱼研究业务，也将减少该设施对环境的影响。