Smart ventilated cage systems for next-level experimental design and monitoring of specialized animal models.

智能通风笼系统，用于下一级实验设计和专门动物模型的监测。

基本信息

批准号：
10737361
负责人：
BRIAN KAROLEWSKI
金额：
$ 33.8万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-07-01 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10737361
关键词：
Address Animal Experiments Animal Housing Animal Model Animal Technicians Animal Welfare Animals Area Attention Beds Behavior Biology Breeding Cancer Model Caring Censuses Circadian Rhythms Clinical Collection Communicable Diseases Complement Complex Darkness Data Data Collection Data Correlations Dedications Disease Disease Progression Disease model Early identification Electrodes Ensure Environmental Monitoring Experimental Designs Fertility Floods Food Generations Genetic Health Health Status House mice Housing Human Humidity Image Individual Infection Investments Knockout Mice Light Lighting Link Locomotion Maintenance Mammals Masks Medical center Medicine Modeling Monitor Mouse Strains Mus Phenotype Physiology Play Precision therapeutics Preclinical Testing Predisposition Prevention strategy Procedures Protocols documentation Reproducibility Research Research Personnel Research Project Grants Research Support Resource Sharing Resources Sampling Schedule Services Site Standardization Stress Symptoms System Temperature Testing Time Transgenic Organisms United States National Institutes of Health Universities Veterinarians Water animal facility animal resource behavioral study circadian comparative digital experimental study human disease improved member mouse model novel operation patient derived xenograft model pre-clinical precision oncology prevent programs remote monitoring sensor treatment response treatment strategy ventilation vibration wasting web interface welfare

项目摘要

PROJECT SUMMARY Murine models play a key role in a broad range of research areas given their comparable physiology to humans and other mammals, known genetic backgrounds, and the ease of their housing and maintenance. However, as we have gained a greater understanding of physiology, there has been a drive to create more sophisticated models that better represent human disease progression and response to treatments. This has led to the generation and use of mouse models with unique genetic backgrounds, behaviors, or susceptibility to diseases. While these models have been of enormous value, they require regular monitoring and may need special housing conditions (such as altered light/dark cycles) to maintain their health and successfully carry out needed research. Incorporating this monitoring adds significant operational burden on facility staff and researchers, and may also add to animal stress. Furthermore, even with increased physical monitoring of mice, important changes in health status can be missed during non-observational periods, making this a challenging problem to address. To address these issues, the Institute of Comparative Medicine (ICM) at Columbia University Irving Medical Center will acquire and install Tecniplast Digital Ventilated Cages (DVC) and Rack Environmental Monitoring modules for its specialized animal resources. The features of the DVC system will improve ICM operations by: 1) Providing automated, 24/7 remote monitoring of various parameters linked to animal welfare and environmental conditions of the animal facility; 2) triggering of automated alarms in particular conditions requiring immediate attention (e.g., decreased spontaneous mouse activity, water flooding, limited water bottle or food availability); 3) setting specific light/dark cycle for individual cages with the use of LEDDY units; and 4) reducing the number of cage changes needed in some circumstances (e.g. single-housed mice), reducing bedding waste. Implementation of DVC cages will be targeted toward specialized shared resources that utilize the most complex disease models, which will lead to a disproportionately large impact on animal welfare, facility operations, experimental capability of researchers, and the reproducibility and rigor of research within ICM.

项目概要鉴于小鼠模型的生理学与小鼠模型相似，因此在广泛的研究领域中发挥着关键作用。人类和其他哺乳动物、已知的遗传背景以及其居住和维护的便利性。然而，随着我们对生理学有了更深入的了解，我们就有动力去创造更多复杂的模型可以更好地代表人类疾病的进展和对治疗的反应。这有导致了具有独特遗传背景、行为或易感性的小鼠模型的产生和使用疾病。虽然这些模型具有巨大的价值，但它们需要定期监控，并且可能需要特殊的住房条件（例如改变光/暗周期）以维持他们的健康并成功地进行需要研究。纳入这种监控给设施工作人员增加了巨大的运营负担研究人员认为，这也可能会增加动物的压力。此外，即使增加了对小鼠的身体监测，在非观察期间可能会错过健康状况的重要变化，这使得这是一项具有挑战性的工作需要解决的问题。为了解决这些问题，哥伦比亚大学欧文医学院比较医学研究所 (ICM) 中心将购买并安装 Tecniplast 数字通风笼 (DVC) 和机架环境监测其专业动物资源的模块。 DVC 系统的功能将通过以下方式改善 ICM 操作： 1) 提供与动物福利相关的各种参数的自动化、24/7 远程监控动物设施的环境条件； 2）特定条件下触发自动警报需要立即注意（例如，小鼠自发活动减少、水淹、水瓶有限或食物供应情况）； 3) 使用 LEDDY 装置为各个笼子设置特定的光/暗周期；和 4) 减少某些情况下（例如单养小鼠）所需的笼子更换次数，减少床上用品废物。 DVC 笼的实施将针对利用最多的专门共享资源复杂的疾病模型，这将对动物福利、设施造成不成比例的巨大影响 ICM 内的操作、研究人员的实验能力以及研究的可重复性和严谨性。