Core--Neurobehavioral Assessment

核心--神经行为评估

基本信息

批准号：
7538389
负责人：
Thomas M Burbacher
金额：
$ 6.85万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7538389
关键词：
A Mouse Acoustics Address Adrenergic Agents Affect Age Aggressive behavior Air Animal Model Animal Testing Animals Appearance Area Atropine Auditory Back Beds Behavior Behavioral Behavioral Paradigm Birth Boxing Caliber Canned Foods Characteristics Cholinergic Agents Cholinergic Receptors Cholinesterase Inhibitors Chronic Cognitive Computer software Conditioned Stimulus Core Facility Cues Daily Defecation Depth Development Devices Disruption Distal Drops Ear Environment Equilibrium Equipment Evaluation Event Experimental Designs Exposure to External Ear Eye Face Fatigue Figs - dietary Floor Food Food deprivation (experimental)Freezing Frequencies Fright Functional disorder Funding Genes Genetic Genetic Crossing Over Genetic Polymorphism Genetic Predisposition to Disease Goals Growth Hair Head Health Health Sciences Health behavior Heating Hindlimb Home environment House mice Housing Hybrids IACUC Inbred Strain Inbred Strains Mice Incubators Individual Isoflurophate Journals Knockout Mice Laboratories Laboratory Rat Lateral Learning Left Leg Length Lifting Light Location Locomotion Maze Learning Measurement Measures Medicine Memory Mental Depression Methodology Methods Milk Molecular Monitor Motivation Motor Motor Activity Movement Mus Muscarinic Antagonists Neonatal Mortality Neuraxis Neurosciences New York Nociception Noise Numbers Odors Organophosphates Paint Paper Paste substance Performance Pesticides Phenotype Physiologic pulse Pigmentation physiologic function Powder dose form Principal Investigator Procedures Process Progress Reports Protocols documentation Psychopharmacology Pulse taking Quantitative Trait Loci Radial Rate Rattus Recommendation Records Reflex action Reporting Research Research Personnel Research Project Grants Resistance Rodent Sampling Schizophrenia Screening procedure Sensory Serotonin Services Shock Short-Term Memory Side Signal Transduction Specific Pathogen Frees Speed Standards of Weights and Measures Stimulus Supine Position Surface Swimming Symptoms System Tactile Temperature Teratology Testing Time Tissues Toxicology Training Transgenic Organisms Travel Upper arm Variant Visual Water Week Wood material Work adrenergic animal facility auditory stimulus behavior test brain research cholinergic comparative computer program conditioned fear conditioning day developmental neurotoxicity experience external ear auricle foot germ free condition handbook instrument lateral ventricle memory process morris water maze neurobehavioral novel pesticide exposure postnatal prepulse inhibition prevent programs pup receptor research study response sensory system size sound stem time interval tool toxicant

项目摘要

Description (provided by applicant): The overall goal of this core is to conduct studies aimed at defining the effects of chronic low-level pesticide exposure on neurobehavioral development in mice. The Behavioral Toxicology Core will provide services for two research projects, one involving pre-natal exposure to pesticides, and the other early postnatal exposure. The Core will provide the capability of evaluating the functional development of the central nervous system, through the use of a Developmental Neurotoxicity Test Battery. This test battery includes measures of motor coordination, reflex responses, nociception, learning and memory, and the appearance of developmental landmarks in mice. The exact tests to be used for the Genetic Susceptibility study will be determined by the results obtained with the PON1-/- and PON1+/+ mice in the current period of support. For example, there were significant differences among treatment groups in the Morris water maze and open field tests (see progress report for the PON1 Polymorphisms study). Thus, tests which are determined by the current studies not to be affected by OP exposure (e. g., possibly the rotarod and radial arm maze tests) might be replaced by other behavioral tests, such as the platform reversal or delayed-matching-to- sample variants of the Morris water maze or a variant of the open field test that examines response to a novel object. The decision of which tests to include in the battery will hinge on the results obtained with the mice currently under neurobehavioral assessment. The Neurobehavioral Assessment Facility is a specific pathogen free (SPF) laboratory located on the fifth floor of the Health Sciences Building, close to the central animal facility and to several of the Center investigator laboratories. The facility is comprised of three rooms (273 sq ft, 78 sq ft and 53 sq ft), with space for housing up to 25 cages of SPF mice. The Department of Comparative Medicine's central facility, located just upstairs from the Behavioral Toxicology Facility, is an AAALAC-accredited SPF facility that has ample space for mouse housing. The Neurobehavioral Assessment Facility was first established in the current funding period to address the specific aims of the Center. Into the early part of 2001, an abnormally high neonatal mortality rate had been preventing expansion of the colony to a size large enough to begin behavioral testing. This difficulty was overcome by moving the mouse housing rooms and behavioral testing laboratory from an off-campus building to the Health Sciences Building, and by implementing SPF procedures in these rooms. This strategy has eliminated the high neonatal mortality that the investigators had been struggling with at the off-campus facility, and has the added advantage of placing the Neurobehavioral Toxicology Facility near the other Center laboratories in the Health Sciences Building. The facility was inspected and approved by the UW IACUC and veterinary services on January 2,2002. Behavioral studies commenced immediately after approval.

描述（由申请人提供）：该核心的总体目标是进行针对的研究定义慢性低水平农药暴露对小鼠神经行为发育的影响。行为毒理学核心将为两个研究项目提供服务，一个涉及产前暴露于农药，另一个早期产后接触。核心将通过使用发育神经毒性测试电池来评估中枢神经系统的功能发展的能力。该测试电池包括运动协调，反射反应，伤害感受，学习和记忆以及小鼠发育地标的外观。用于遗传易感性研究的确切测试将取决于当前支持期间用PON1 - / - 和PON1+/+小鼠获得的结果。例如，莫里斯水迷宫和开放式测试的治疗组之间存在显着差异（请参阅PON1多态性研究的进度报告）。因此，由当前研究确定的测试不受OP暴露影响（例如，可能是rotarod和radial臂迷宫测试）可能会被其他行为测试所取代，例如平台逆转或延迟匹配到 - 莫里斯水迷宫的样品变体或开放田测试的变体，检查对新物体的反应。在电池中包含哪些测试的决定将取决于当前在神经行为评估下的小鼠获得的结果。神经行为评估设施是一个特定的无病原体（SPF）实验室，位于健康科学大楼五楼，靠近中央动物设施和几个中心研究员实验室。该设施由三个房间（273平方英尺，78平方英尺和53平方英尺）组成，可容纳多达25个笼子的SPF小鼠。比较医学部的中央设施位于行为毒理学设施的楼上，是AAALAC认可的SPF设施，具有足够的小鼠住房空间。神经行为评估设施首先在当前的资金期间建立，以解决该中心的具体目标。到2001年初，异常高的新生儿死亡率一直在阻止菌落扩大到足以开始行为测试的大小。通过将小鼠住房室和行为测试实验室从校外建筑物转移到健康科学大楼以及在这些房间中实施SPF程序，克服了这一困难。该策略消除了调查人员在校外设施中一直在挣扎的新生儿死亡率，并具有将神经行为毒理学设施放置在健康科学大楼其他中心实验室附近的额外优势。该设施于2002年1月2日由UW IACUC和兽医服务检查和批准。批准后立即开始行为研究。