Multi-spectral Mosaic (MSM) Digital Imaging Sensor for Microscopy Applications

适用于显微镜应用的多光谱马赛克 (MSM) 数字成像传感器

• 批准号: 7407933
• 负责人: Steve Michael Savoy
• 金额: $ 25.14万
• 依托单位: NANOHMICS, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-21 至 2010-07-20
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7407933
• 关键词: Algorithms; Binding; Biomedical Engineering; Collaborations; Color; Cost Effectiveness Analysis; Cypress; Data; Deposition; Development; Devices; Diagnostic; Digital Photography; Dyes; Elements; Fluorescent in Situ Hybridization; Gray unit of radiation dose; Image; Immobilization; In Situ Hybridization; Karyotype determination procedure; Lead; Libraries; Light; Liquid substance; Methods; Microarray Analysis; Microfluidics; Microscopy; Microspheres; Numbers; Oligonucleotide Microarrays; Oligonucleotides; Optics; Pattern; Phase; Polymers; Preparation; Process; Range; Reproduction; Research; Resolution; Semiconductors; Speed; Standards of Weights and Measures; Streptavidin; Surface; System; Techniques; Technology; Testing; Tissues; Ultrafine; computerized data processing; concept; cost; density; design; digital imaging; instrument; instrumentation; next generation; novel; particle; seal; sensor

DESCRIPTION (provided by applicant): Compact, low-cost digital imaging systems that combine the benefits of high spatial and spectral resolution are in demand for bioanalytical analyses such as in situ hybridization (ISH) and fluorescence in situ hybridization (FISH) karyotyping, tissue and cellular diagnostics, and microarrays. Advances in commercial image sensors have led to pixel densities which provide exquisite spatial resolution. Despite pixel densities sufficient to resolve ultrafine features with large fields of view, commercially available color CMOS cameras are still limited in the level of spectroscopic (chromaticity) resolution. Color imaging systems are generally restricted by the Bayer color filter array (CFA) mosaic patterning (i.e. usually RGB three color filter arrays (CFAs). These color camera chips are designed to meet minimal color reproduction requirements for digital photography. Higher spectroscopic band definition is achieved through combination of a monochrome image sensor with dispersive/filter elements that are bulky and expensive, particularly for those involving motorized switching between filter sets. Likewise, electronically tunable filters (e.g. LCD, acousto-optic, Sagnac, etc.) that slow image acquisition speed are not ideal. Elimination of external dispersive elements and slow tunable filters requires directly integrating higher spectral definition into the mosaic pattern on the image sensor (CMOS/CCD). Nanohmics, in collaboration with Atactic Technologies, propose to develop a process for expanding the spectral resolution and range (e.g. UV, visible and near-IR) of Color Filter Arrays (CFAs) directly on the CMOS sensor surface. Furthermore, these Multi-Spectral Mosaic (MSM) patterns will be deposited using a novel method that utilizes the lock-and- key registration of oligonucleotide micorarrays to direct surface immobilization of core- dyed microspheres into the mosaic pattern on the image sensor. The proposed method is vastly different than standard serial lithographic processing currently used in commercial preparation of existing color camera CFAs. The proposed oligo-directed immobilization will lead to the development of the entire mosaic pattern in a single processing step. This proposed technique was exploit biomolecular recognition to bioengineer the material layer in a commercial device application.Project Narrative The expansion of spectral capabilities of a compact image sensor would enable more rapid and cost effective analyses of biomolecular processes in a number of fields including microarray analysis, diagnostics, and karyotyping. Furthermore, enhanced spectral resolution will lead to higher color fidelity reproduction for all image sensor applications. The use of biomolecular materials for bioengineering is an important enabled feature of the proposed research

描述（由申请人提供）：原位杂交 (ISH) 和荧光原位杂交 (FISH) 核型分析、组织分析等生物分析需要紧凑、低成本的数字成像系统，该系统结合了高空间和光谱分辨率的优点。以及细胞诊断和微阵列。商业图像传感器的进步提高了像素密度，从而提供了精致的空间分辨率。尽管像素密度足以解析大视场的超精细特征，但商用彩色 CMOS 相机的光谱（色度）分辨率水平仍然受到限制。彩色成像系统通常受到拜耳滤色器阵列 (CFA) 马赛克图案（即通常为 RGB 三滤色器阵列 (CFA)）的限制。这些彩色相机芯片旨在满足数字摄影的最低色彩再现要求。更高的光谱带清晰度通过将单色图像传感器与体积庞大且昂贵的色散/滤光片元件相结合来实现，特别是对于那些涉及滤光片组之间的电动切换的滤光片。同样，电子可调滤光片（例如 LCD、声光、 Sagnac 等）认为，消除外部色散元件和慢速可调滤光片需要与 Atropic Technologies 合作，将更高的光谱清晰度直接集成到图像传感器 (CMOS/CCD) 上的马赛克图案中。 ，建议开发一种工艺，直接扩展 CMOS 传感器表面上的彩色滤光片阵列 (CFA) 的光谱分辨率和范围（例如紫外、可见光和近红外）。此外，这些多光谱马赛克（MSM）图案将使用一种新颖的方法进行沉积，该方法利用寡核苷酸微阵列的锁钥匙配准，将核心染色微球的表面固定到图像传感器上的马赛克图案中。所提出的方法与目前在现有彩色相机 CFA 的商业制备中使用的标准串行光刻处理有很大不同。所提出的寡核苷酸定向固定将导致在单个处理步骤中形成整个镶嵌图案。这项提出的技术是利用生物分子识别对商业设备应用中的材料层进行生物工程。项目叙述 紧凑型图像传感器的光谱能力的扩展将能够在包括微阵列分析、诊断和核型分析在内的许多领域中对生物分子过程进行更快速且更具成本效益的分析。此外，增强的光谱分辨率将为所有图像传感器应用带来更高的色彩保真度再现。将生物分子材料用于生物工程是该研究的一个重要特征