Label-Free NanOFET Microarrays with Embedded-Probe Nanocomposite Active Layers

具有嵌入式探针纳米复合活性层的无标记 NanoOFET 微阵列

• 批准号: 7054350
• 负责人: Steve Michael Savoy
• 金额: $ 39.93万
• 依托单位: NANOHMICS, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7054350
• 关键词: biotechnology; genetic library; high throughput technology; microarray technology; nanotechnology; nucleic acid probes; polymers; semiconduction; technology /technique development; temperature

DESCRIPTION (provided by applicant): Nanohmics, Inc. and Ambion, Inc. working in consortium with Dr. Susan Kauzlarich at the University of California Davis and Dr. Lynn Loo at the University of Texas at Austin propose to develop a Nanocomposite Organic Field-Effect Transistor (NanOFET) multiplex microarray platform for compact, label-free detection of oligonucleotide assays. The proposed method will involve the development of ultrathin, high carrier mobility, polymeric/semiconductor composites consisting of doped nanoparticles and conducting polymers. The proposed geometry provides a means to measuring both charge effects and localized temperature change resulting from the hybridization reaction. Nucleotide anchors will be immobilized to the surface of the nanoparticles and be embedded in the composite active layer. These organized composite active layers will be deposited using a single step transfer technique over the entire surface of the NanOFET array. Lithographically patterned source and drain contacts will serve as independent immobilization sites for extension of each probe oligonucleotide in the library. The specific aim of the proposed research is to develop a high throughput analysis platform that does not require a secondary labeling step. In this regard, microarray analysis with multiple targets can be performed with a single sample aliquot and be analyzed using compact reader devices. The proposed method will involve the first attempt to integrate oligo photocoupling chemistry with a field effect transistor array in the preparation of active structures with embedded probe anchors. The development of FET geometry with the proposed novel active semiconducting layer will provide a means for scaling FET arrays to production microarray devices as opposed to other geometries that have limited scalability. The microarray will operate as a label-free, direct electrical transduction platform and be analyzed by compact reader instrumentation, including field applications, where it may be possible to perform real time disease state, pathogen or biothreat detection on oligonucleotide samples.

描述（由申请人提供）：Nanohmics, Inc. 和 Ambion, Inc. 与加州大学戴维斯分校的 Susan Kauzlarich 博士和德克萨斯大学奥斯汀分校的 Lynn Loo 博士合作，提议开发纳米复合有机场 -效应晶体管 (NanOFET) 多重微阵列平台，用于紧凑、无标记的寡核苷酸检测检测。所提出的方法将涉及开发由掺杂纳米颗粒和导电聚合物组成的超薄、高载流子迁移率聚合物/半导体复合材料。所提出的几何结构提供了一种测量杂化反应引起的电荷效应和局部温度变化的方法。核苷酸锚将固定在纳米颗粒的表面并嵌入复合活性层中。这些有组织的复合有源层将使用单步转移技术沉积在 NanOFET 阵列的整个表面上。光刻图案化的源极和漏极触点将作为文库中每个探针寡核苷酸延伸的独立固定位点。拟议研究的具体目标是开发一个不需要二次标记步骤的高通量分析平台。在这方面，可以使用单个样品等分试样进行具有多个靶标的微阵列分析，并使用紧凑型读取器设备进行分析。所提出的方法将首次尝试将寡聚光耦合化学与场效应晶体管阵列集成，以制备具有嵌入式探针锚的有源结构。与具有有限可扩展性的其他几何结构相比，采用所提出的新型有源半导体层的 FET 几何结构的开发将提供一种将 FET 阵列扩展为生产微阵列器件的方法。该微阵列将作为无标记的直接电转导平台运行，并通过紧凑型读取器仪器进行分析，包括现场应用，其中可以对寡核苷酸样品进行实时疾病状态、病原体或生物威胁检测。