Cell Culture and Engineering Core (CCEC)

细胞培养与工程核心（CCEC）

基本信息

批准号：
10693936
负责人：
OWEN M WOODWARD
金额：
$ 18.72万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-24 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10693936
关键词：
3-Dimensional Academia Acceleration Address Affect Alleles Antibodies Autosomal Dominant Polycystic Kidney Biological Assay Cell Culture Techniques Cell Line Cell model Cells Cellular Assay Clinical Clinical Data Collaborations Communities Consultations Coupled Custom Cyst Cyst Fluid Data Duct (organ) structure Ductal Epithelial Cell Engineering Ensure Enterobacteria phage P1 Cre recombinase Epithelial Cells Experimental Designs Future Gene Expression Gene Expression Profiling Gold Government Human In Vitro Individual Industry Investigation Kidney Knock-out Knockout Mice LoxP-flanked allele Maryland Modeling Mus Mutation Organoids Pathway interactions Patients Phenotype Polycystic Kidney Diseases Positioning Attribute Preparation Primary Cell Cultures Procedures Proliferating Proprotein Convertase 1 Protocols documentation Quality Control Reproducibility Research Research Personnel Resources Services Site Specimen Stretching System Tetracyclines Therapeutic Tissues Translations Validation cellular engineering design frontier high standard high throughput screening in vitro Model inducible Cre innovation interest kidney epithelial cell knock-down mutant new technology organizational structure renal epithelium screening single nucleus RNA-sequencing therapeutic evaluation treatment response validation studies vector

项目摘要

PROJECT SUMMARY The Maryland Polycystic Kidney Disease Research and Translation Core Center (MPKD-RTCC) Cell Culture and Engineering Core (CCEC) will accelerate progress in PKD research by addressing a major unmet need for well-defined, reliable in vitro cell PKD models with proper controls. With this overarching goal in mind, CCEC developed new in vitro cell and organoid models that we hope will become the “gold-standards” of the field. The CCEC is committed to helping investigators find ideal solutions for all their individual in vitro research needs whether it involves mechanistic exploration, human PKD cell validation studies, or therapeutic screening. To best serve the current and expected future demands of the diverse PKD workforce in academia, government, and industry, the CCEC will provide murine cell models of ADPKD, provide engineered inner medullary collecting duct (IMCD3) cell lines expressing custom PKD1 and PKD2 mutations, provide human kidney ADPKD cells and specimens, provide a cutting edge cystogenesis assay using three-dimensional organoid system, provide high through-put, three- dimensional cyst expansion assay, and provide consultation services to make sure the PKD community can fully utilize the CCEC resources.

项目摘要马里兰州多囊肾脏疾病研究与翻译核心中心（MPKD-RTCC）细胞培养和工程核心（CCEC）将通过满足对具有正确定义，可靠的体外细胞PKD模型的主要未满足的需求控件。考虑到这个总体目标，CCEC开发了新的体外细胞和器官我们希望的模型将成为该领域的“金牌标准”。 CCEC致力于帮助调查人员为所有个人的体外研究找到理想的解决方案是否需要涉及机械探索，人类PKD细胞验证研究或治疗性筛查。到最佳服务于学术界的潜水员PKD劳动力的当前和预期的未来需求，政府和行业，CCEC将提供ADPKD的鼠细胞模型，提供设计的内部髓质收集管（IMCD3）表达自定义PKD1和PKD2的细胞系突变，提供人类肾脏ADPKD细胞和标本，提供尖端使用三维器官系统的囊肿测定维囊肿扩展测定法，并提供咨询服务以确保PKD 社区可以充分利用CCEC资源。