Swine Somatic Cell Genome Editing (SCGE) Center

猪体细胞基因组编辑（SCGE）中心

• 批准号: 10655827
• 负责人: RANDALL S PRATHER
• 金额: $ 6.61万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-06-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10655827
• 关键词: Animal Model; Clinic; Collaborations; Data; Data Collection; Data Coordinating Center; Ensure; Evaluation; Family suidae; Freezing; Future; Genome; Histologic; Human; Individual; Laboratories; Methods; Modeling; Mus; Physiological; Preparation; Procedures; Production; Reagent; Reporter; Reporting; Role; Safety; Sampling; Sampling Studies; Technology; Technology Transfer; Testing; Tissues; Translating; Translations; Work; animal data; data integrity; data sharing; efficacy study; genome editing; member; method development; novel; porcine model; preservation; reagent testing; safety study; somatic cell gene editing

Project Summary/Abstract The role of the Genome Editing Testing Section is to validate unique reporter models created by the Animal Model Production Section and to test novel delivery methods and reagents provided by other members of the Somatic Cell Genome Editing (SCGE) Consortium. Additionally, the Genome Editing Testing Section is responsible for creating standard operating procedures (SOPs) and comprehensive reports for testing new genome editing technologies. The purpose of this proposal is to test the "second-round" of reagents that have been proven in the mouse and targeted for evaluation in pigs. In preparation for translating delivery and editing technologies to the clinic, we aim to complete safety and efficacy studies in a swine model. Further we will be responsible for preservation of samples from these studies in anticipation of future needs.

项目概要/摘要 基因组编辑测试部分的作用是验证由基因组编辑创建的独特报告模型 动物模型制作科并测试其他机构提供的新型递送方法和试剂 体细胞基因组编辑 (SCGE) 联盟的成员。此外，基因组编辑 测试部门负责创建标准操作程序（SOP）和全面的 测试新基因组编辑技术的报告。该提案的目的是测试 “第二轮”试剂已在小鼠中得到验证，并针对猪进行评估。在 准备将交付和编辑技术转化为临床，我们的目标是完成安全和 猪模型的功效研究。此外，我们将负责保存来自 这些研究是为了预测未来的需求。