Research Project for NSRRC Renewal Year 21-25

NSRRC 续期研究项目 21-25

• 批准号: 10553793
• 负责人: RANDALL S PRATHER
• 金额: $ 15.04万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10553793
• 关键词: Anatomy; Applied Research; Arteriosclerosis; Biological Assay; Biomedical Research; Cardiovascular Diseases; Characteristics; Client; Communicable Diseases; Communities; Country; Cryopreservation; Development; Diabetes Mellitus; Disease; Domestic Pig; Embryo; Embryo Transfer; Ensure; Equipment and supply inventories; Family suidae; Funding; Future; Genetic Engineering; Genetic Materials; Germ Cells; Goals; Health; Human; Knock-out; Medical Device; Medical Research; Methods; Modeling; Modification; Molecular; Monitor; Optics; Organ; Performance; Pharmaceutical Preparations; Physiological; Physiology; Production; Reagent; Research; Research Personnel; Research Project Grants; Resources; Scientist; Study models; Technology; Tissues; United States; Work; Xenograft procedure; embryo cryopreservation; experience; human disease; improved; innovation; method development; novel therapeutic intervention; operation; pig genome; porcine model; pre-clinical; prevent; repository; reproductive; sex; somatic cell nuclear transfer; sperm cell; tool; web-accessible

Abstract (Applied Research) The overall goal of this application is to continue and expand operation of the National Swine Resource and Research Center (NSRRC). During the past funding period, the NSRRC developed a non-invasive method of cryopreservation of embryos via ultra-fast cooling, improved methods for the development of somatic cell nuclear transfer embryos, developed a universal sex determining assay, and incorporated a GHR knockout in an attempt to reduce the size of domestic pigs while retaining their superior reproductive performance, and developed state-of-the-art disease monitoring and surveillance assays. The Specific Aim of the Research Core is to perform innovative research that will benefit the NSRRC and the biomedical research community. Applied research projects are aimed at improving a) the cryopreservation of pig reproductive cells, and b) the development of improved methods for the production of genetically-engineered pigs.

摘要（应用研究） 该应用程序的总体目标是继续并扩大国家生猪资源和 研究中心（NSRRC）。在过去的资助期间，NSRRC 开发了一种非侵入性方法 通过超快速冷却冷冻保存胚胎，改进体细胞发育方法 核移植胚胎，开发了通用性别决定分析，并纳入了 GHR 敲除 试图缩小家猪的体型，同时保留其优越的繁殖性能， 并开发了最先进的疾病监测和监测分析方法。具体目标 研究核心是进行有利于 NSRRC 和生物医学研究的创新研究 社区。应用研究项目旨在改进 a) 猪生殖能力的冷冻保存 b) 开发生产基因工程猪的改进方法。