Can high pressure processing (HPP) and ultraviolet-C irradiation (UV-C) treatment preserve donor milk bioactive protein structure and function better than holder pasteurization?

高压处理 (HPP) 和紫外线 C 照射 (UV-C) 处理能否比巴氏灭菌更好地保留供乳生物活性蛋白的结构和功能？

• 批准号: 10655572
• 负责人: David Charles Dallas
• 金额: $ 37.25万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10655572
• 关键词: Address; Adhesives; Affect; Aftercare; Anti-Bacterial Agents; Biological; Biological Assay; Ensure; Enzyme-Linked Immunosorbent Assay; Goals; Growth; Health; Human Milk; Immune system; Immunoglobulins; In Vitro; Infant; Infant Development; Infant Health; Infection; Lactoferrin; Lipase; Methods; Milk; Milk Banks; Milk Proteins; Mothers; Muramidase; Nutritional; Nutritional Study; Outcome; Peptide Hydrolases; Predisposition; Premature Infant; Process; Proteins; Proteomics; Research; Risk; Role; Safety; Shapes; Source; Techniques; Viral; Work; bile salts; donor milk; feeding; immunoregulation; improved; infant nutrition; irradiation; microbiome; nutrient absorption; nutrition; pasteurization; pathogen; preservation; pressure; programs; protein function; protein structure; ultraviolet; unpasteurized

Human milk bioactive proteins are degraded during commonly used Holder pasteurization of donor milk. Alternative processing techniques that ensure biosafety while preserving bioactive proteins are needed, particularly for at-risk preterm infants. High pressure processing (HPP) and ultraviolet-C irradiation (UV-C) treatment are known to preserve a few bioactive milk proteins, but no systematic research has identified the minimum processing parameters and their effects on the entire array of milk proteins’ structure and function. There is a critical need to perform this research. Our long-term goal is to optimize feeding practices for preterm infants to improve their health outcomes. The objectives of this research are to identify the minimum HPP and UV-C treatment conditions that achieve equivalent microbiological safety to Holder pasteurization while optimally preserving bioactive protein structure and function. Our central hypothesis is that minimal HPP and UV-C treatment conditions will better preserve donor milk bioactive proteins’ structure and function compared with Holder pasteurization. Our hypothesis is based on our work and that of others indicating that HPP and UV- C treatment preserve some bioactive proteins. The rationale for this work is that it can lead to changes in donor milk processing that can improve bioactive protein retention and possibly infant health outcomes. Aim 1. Determine treatment conditions for HPP and UV-C pasteurization that maximize bioactive protein preservation compared with Holder pasteurization. Minimal conditions for HPP and UV-C biosafety will be determined and retention of bioactive milk proteins will be compared between unpasteurized, Holder pasteurized, HPP-treated and UV-C-treated donor milk via ELISA and proteomics analyses. Our working hypothesis is that minimal-condition HPP and UV-C-treated donor milk will have higher retention of bioactive proteins than Holder pasteurized donor milk. Aim 2. Identify the extent to which preserved bioactive proteins maintain their bioactivities after treatment with HPP and UV-C pasteurization. Bioactivity will be examined in whole milk and fractionated protein extracts of unpasteurized and Holder, HPP and UV-C pasteurized donor milk. Our working hypothesis is that HPP and UV-C treated donor milk proteins will retain a higher degree of their bioactivities compared with Holder pasteurized donor milk as determined by in vitro antibacterial, anti-adhesive, antiviral and immunomodulatory assays, lipase and protease assays. We expect to have determined the extent to which minimally processed HPP and UV-C treatment preserves bioactive proteins’ structure and function compared with Holder pasteurization. The positive impact of this research will be guidance for donor milk processors on how to optimally process donor milk for feeding preterm infants and information for clinicians on how to evaluate available donor milk sources. Changes in milk processing to better preserve bioactive milk proteins could improve preterm infant health outcomes.

母乳生物活性蛋白在常用的供体母乳巴氏灭菌过程中会被降解，因此需要在保留生物活性蛋白的同时确保生物安全的替代加工技术，特别是对于高危早产儿而言。 C) 众所周知，处理可以保留一些生物活性的乳蛋白，但还没有系统的研究确定最低加工参数及其对整个乳蛋白结构和功能的影响，因此迫切需要进行这项研究。我们的长期目标是优化早产儿的喂养方法，以改善他们的健康状况。本研究的目的是确定最低 HPP 和 UV-C 处理条件，以达到与 Holder 巴氏灭菌相当的微生物安全性，同时最佳地保留生物活性蛋白质结构。我们的中心假设是，与 Holder 巴氏灭菌相比，最小的 HPP 和 UV-C 处理条件将更好地保留供体乳生物活性蛋白的结构和功能。我们的假设是基于我们和其他人的工作，表明 HPP 和 UV-C 处理。治疗保留这项工作的基本原理是，它可以改变捐赠母乳的加工过程，从而改善生物活性蛋白的保留，并可能改善婴儿的健康结果。 目标 1. 确定 HPP 和 UV-C 巴氏灭菌的处理条件，以最大限度地保存生物活性蛋白。将确定 HPP 和 UV-C 生物安全性的最低条件，并对未经巴氏灭菌、Holder 巴氏灭菌、HPP 处理和 UV-C 处理之间生物活性乳蛋白的保留进行比较。我们的工作假设是，经过最低条件 HPP 和 UV-C 处理的供体奶比经过 Holder 巴氏灭菌的供体奶具有更高的生物活性蛋白质保留率。 目标 2. 确定保留的生物活性蛋白质的保留程度。我们将检查未经巴氏灭菌和 Holder、HPP 和 UV-C 巴氏灭菌的全脂牛奶和分馏蛋白质提取物经过 HPP 和 UV-C 巴氏灭菌后的生物活性。假设是，与 Holder 巴氏灭菌的供乳蛋白相比，经过 HPP 和 UV-C 处理的供乳蛋白将保留更高程度的生物活性，这是通过体外抗菌、抗粘附、抗病毒和免疫调节测定、脂肪酶和蛋白酶测定确定的。确定了与 Holder 巴氏灭菌相比，最低限度加工的 HPP 和 UV-C 处理在多大程度上保留了生物活性蛋白质的结构和功能。这项研究的积极影响将为捐赠母乳提供指导。加工者如何优化处理用于喂养早产儿的捐赠母乳，以及为狂热分子提供如何评估可用捐赠母乳来源的信息，以更好地保存生物活性乳蛋白可以改善早产儿的健康结果。

项目成果

Summary of the Joint National Institutes of Health and the Food and Drug Administration Workshop Titled "Exploring the Science Surrounding the Safe Use of Bioactive Ingredients in Infant Formula: Considerations for an Assessment Framework".

• DOI: 10.1016/j.jpeds.2022.11.027
• 发表时间: 2023-04
• 期刊: JOURNAL OF PEDIATRICS
• 影响因子: 5.1
• 作者: Donovan, Sharon M.;Abrams, Steven A.;Azad, Meghan B.;Belfort, Mandy B.;Bode, Lars;Carlson, Susan E.;Dallas, David C.;Hettinga, Kasper;Jarvinen, Kirsi;Kim, Jae H.;Lebrilla, Carlito B.;McGuire, Michelle K.;Sela, David A.;Neu, Josef
• 通讯作者: Neu, Josef