Genomics, Bioinformatics, and Molecular Imaging Core

基因组学、生物信息学和分子成像核心

• 批准号: 10414532
• 负责人: Malwina Czarny-Ratajczak
• 金额: $ 54.49万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10414532
• 关键词: Aging; Applications Grants; Bioinformatics; Biological Assay; Biomedical Research; Biometry; Biostatistics Core; Cancer Center; Capillary Electrophoresis; Cell Line; Cells; Center for Translational Science Activities; Centers of Research Excellence; Chromium; Client; Clinical Sciences; Communities; Computational Biology; DNA; DNA sequencing; Data; Data Analyses; Data Analytics; Development; Dideoxy Chain Termination DNA Sequencing; Epidemiology; Equipment; Experimental Designs; Funding; Gene Expression; Genes; Genomics; Genotype; Goals; Growth; Human Resources; Louisiana; Luciferases; Measurement; Measures; Mentors; Microsatellite Repeats; Molecular; Names; Peer Review; Phase; Principal Investigator; Productivity; Proteomics; Publications; Quality Control; RNA; Regenerative Medicine; Research; Research Personnel; Research Project Grants; Services; Source; System; Technology; Time; United States National Institutes of Health; Universities; Validation; XCL1 gene; base; bioinformatics pipeline; design; equipment acquisition; exome; increased appetite; instrumentation; laboratory equipment; miRNA expression profiling; molecular imaging; next generation sequencing; operation; transcriptome; transcriptome sequencing

The objective of the Genomics, Bioinformatics, and Molecular Imaging Core is to support and to enhance the research productivity of both COBRE and non-COBRE investigators. This is measured by our contributions to their peer-reviewed publications and grant applications. Our clients utilize our services from the study planning and design phase through the data analysis and presentation phase. The Core currently provides services for next-generation sequencing (NGS), DNA Sanger sequencing, genotyping, cell line validation, bioinformatics and biostatistics support, as well as other equipment and services. In Phase III, we will further enhance our core and organize its operations under three co-directors who have complementary expertise that encompasses all aspects of services offered. Because of these enhancements, we added ‘Molecular Imaging’ to our moniker. We will pursue the following specific aims: (1) Maintain access and services for next-generation sequencing (RNA- Seq, WES, miRNA-Seq, Ampli-Seq), Sanger DNA sequencing with capillary electrophoresis, real-time PCR applications, measurements for luciferase assays, DNA and RNA isolation, quantitation and quality control of DNA and RNA with the Agilent 2100 Bioanalyzer and cell line validation for investigators. (2) Expand the molecular portfolio of core services and implement new NGS applications by establishing Visium Spatial Gene Analysis using High-plex Proteomics and Whole Transcriptome Analysis, Chromium Single Cell Gene Expression and Multiome ATAC + Gene Expression using 10x Genomics and Illumina systems. (3) Continue biostatistics and bioinformatics support for data generated by the core and outside the core and develop new bioinformatics pipelines. (4) Expand and maintain sustainability and growth of the Core, by implementing recharge to all services and increase it over the course of Phase III and identify and deploy other sources of support. Our enhanced instrumentation and expanded personnel will make this possible, and this will help satiate the ever- increasing appetite for all of these applications. Our data analytic services will allow our clients to receive data that is not only clean and valid quantitatively but also has been put through a bioinformatics pipeline that makes it relevant biologically in broader terms. Both the biostatistics and bioinformatics components of the Core will contribute to the development of new and better approaches to the handling of the data our investigators generate.

基因组学，生物信息学和分子成像核心的目的是支持和增强 研究人员和非近代研究人员的研究生产力。这是通过我们对 他们的同行评审出版物和授予申请。我们的客户从研究计划中利用我们的服务 通过数据分析和演示阶段设计阶段。核心当前为 下一代测序（NGS），DNA Sanger测序，基因分型，细胞系验证，生物信息学和 生物统计学支持以及其他设备和服务。在第三阶段，我们将进一步增强我们的核心和 在三个具有完整专业知识的联合主管下组织其运营 提供服务的各个方面。由于这些增强功能，我们为绰号添加了“分子成像”。我们 将追求以下具体目标：（1）维护下一代测序的访问和服务（RNA- Seq，Wes，miRNA-Seq，Ampli-Seq），用毛细管电泳的Sanger DNA测序，实时PCR 应用，荧光素酶测定的测量，DNA和RNA隔离，定量和质量控制 DNA和RNA具有敏捷2100生物分析仪和研究人员细胞系验证的DNA和RNA。 （2）扩展 核心服务和实施的分子投资组合通过建立森林空间基因来实现新的NGS应用 使用高质量蛋白质组学和整个转录组分析，铬单细胞基因的分析 使用10倍基因组学和Illumina系统的表达和多重ATAC +基因表达。 （3）继续 生物统计学和生物信息学支持由核心和核心外部生成的数据并开发新的数据 生物信息学管道。 （4）通过实施充值来扩展和维持核心的可持续性和增长 为了所有服务，并在第三阶段的过程中增加它，并确定和部署其他支持来源。我们的 增强的仪器和扩大人员将使这可能成为可能，这将有助于不满意 - 增加所有这些应用程序的需求。我们的数据分析服务将使我们的客户接收数据 这不仅是干净且有效的定量，而且还通过生物信息学管道进行了 它在更广泛的角度上相关。核心的生物统计学和生物信息学组成部分都将 有助于开发新的，更好的方法来处理我们的调查人员的数据 产生。