Genomics, Bioinformatics, and Molecular Imaging Core

基因组学、生物信息学和分子成像核心

• 批准号: 10414532
• 负责人: Malwina Czarny-Ratajczak
• 金额: $ 54.49万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10414532
• 关键词: Aging; Applications Grants; Bioinformatics; Biological Assay; Biomedical Research; Biometry; Biostatistics Core; Cancer Center; Capillary Electrophoresis; Cell Line; Cells; Center for Translational Science Activities; Centers of Research Excellence; Chromium; Client; Clinical Sciences; Communities; Computational Biology; DNA; DNA sequencing; Data; Data Analyses; Data Analytics; Development; Dideoxy Chain Termination DNA Sequencing; Epidemiology; Equipment; Experimental Designs; Funding; Gene Expression; Genes; Genomics; Genotype; Goals; Growth; Human Resources; Louisiana; Luciferases; Measurement; Measures; Mentors; Microsatellite Repeats; Molecular; Names; Peer Review; Phase; Principal Investigator; Productivity; Proteomics; Publications; Quality Control; RNA; Regenerative Medicine; Research; Research Personnel; Research Project Grants; Services; Source; System; Technology; Time; United States National Institutes of Health; Universities; Validation; XCL1 gene; base; bioinformatics pipeline; design; equipment acquisition; exome; increased appetite; instrumentation; laboratory equipment; miRNA expression profiling; molecular imaging; next generation sequencing; operation; transcriptome; transcriptome sequencing

The objective of the Genomics, Bioinformatics, and Molecular Imaging Core is to support and to enhance the research productivity of both COBRE and non-COBRE investigators. This is measured by our contributions to their peer-reviewed publications and grant applications. Our clients utilize our services from the study planning and design phase through the data analysis and presentation phase. The Core currently provides services for next-generation sequencing (NGS), DNA Sanger sequencing, genotyping, cell line validation, bioinformatics and biostatistics support, as well as other equipment and services. In Phase III, we will further enhance our core and organize its operations under three co-directors who have complementary expertise that encompasses all aspects of services offered. Because of these enhancements, we added ‘Molecular Imaging’ to our moniker. We will pursue the following specific aims: (1) Maintain access and services for next-generation sequencing (RNA- Seq, WES, miRNA-Seq, Ampli-Seq), Sanger DNA sequencing with capillary electrophoresis, real-time PCR applications, measurements for luciferase assays, DNA and RNA isolation, quantitation and quality control of DNA and RNA with the Agilent 2100 Bioanalyzer and cell line validation for investigators. (2) Expand the molecular portfolio of core services and implement new NGS applications by establishing Visium Spatial Gene Analysis using High-plex Proteomics and Whole Transcriptome Analysis, Chromium Single Cell Gene Expression and Multiome ATAC + Gene Expression using 10x Genomics and Illumina systems. (3) Continue biostatistics and bioinformatics support for data generated by the core and outside the core and develop new bioinformatics pipelines. (4) Expand and maintain sustainability and growth of the Core, by implementing recharge to all services and increase it over the course of Phase III and identify and deploy other sources of support. Our enhanced instrumentation and expanded personnel will make this possible, and this will help satiate the ever- increasing appetite for all of these applications. Our data analytic services will allow our clients to receive data that is not only clean and valid quantitatively but also has been put through a bioinformatics pipeline that makes it relevant biologically in broader terms. Both the biostatistics and bioinformatics components of the Core will contribute to the development of new and better approaches to the handling of the data our investigators generate.

基因组学、生物信息学和分子成像核心的目标是支持和增强 COBRE 和非 COBRE 研究人员的研究生产力是通过我们对研究的贡献来衡量的。 他们的同行评审出版物和资助申请我们的客户利用我们的研究规划服务。 从设计阶段到数据分析和呈现阶段，Core 目前提供的服务包括： 下一代测序 (NGS)、DNA Sanger 测序、基因分型、细胞系验证、生物信息学和 在第三阶段，我们将进一步增强我们的核心和服务。 在三名联合董事的领导下组织其运营，他们拥有涵盖所有方面的互补专业知识 由于这些增强，我们在我们的绰号中添加了“分子成像”。 将追求以下具体目标： (1) 维持下一代测序（RNA- Seq、WES、miRNA-Seq、Ampli-Seq)、毛细管电泳 Sanger DNA 测序、实时 PCR 应用、荧光素酶测定测量、DNA 和 RNA 分离、定量和质量控制 使用 Agilent 2100 生物分析仪和细胞系对研究人员进行 DNA 和 RNA 验证 (2) 展开 通过建立Visium Spatial Gene，提供核心服务的分子组合并实施新的NGS应用 使用高复合蛋白质组学和全转录组分析进行分析，铬单细胞基因 使用 10x Genomics 和 Illumina 系统进行表达和多组 ATAC + 基因表达 (3) 继续。 生物统计学和生物信息学支持核心和核心外生成的数据并开发新的 (4) 通过实施充值来扩大和维持核心的可持续性和增长 所有服务，并在第三阶段中增加它，并确定和部署其他支持来源。 增强的仪器和扩大的人员将使这成为可能，这将有助于满足不断变化的需求。 对所有这些应用程序的兴趣不断增加，我们的数据分析服务将使我们的客户能够接收数据。 这不仅在定量上是干净和有效的，而且已经通过生物信息学管道，使得 核心的生物统计学和生物信息学部分都具有更广泛的生物学意义。 为我们的研究人员开发新的、更好的数据处理方法做出贡献 产生。